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  1. Link

    c1q-antibody-7h8-ab11861.pdf

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Immunology Innate Immunity Complement Classical Pathway
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Anti-C1q 抗体 [7H8] (ab11861)

  • Datasheet
  • SDS
Reviews (9)Q&A (8)References (12)

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Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Immunohistochemistry (Frozen sections) - Anti-C1q antibody [7H8] (ab11861)

    Key features and details

    • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
    • Rat monoclonal [7H8] to C1q
    • Suitable for: ICC/IF, ELISA, IHC-FoFr, ICC, IHC-Fr
    • Reacts with: Mouse, Human

    こちらの製品もご検討ください

    一次抗体
    Product image
    Anti-C3 antibody [11H9] (ab11862)
    一次抗体
    Product image
    Anti-C1q antibody [4.8] (ab182451)
    抗体ペア
    Product image
    Mouse Albumin Matched Antibody Pair Kit (ab210890)

    関連製品

    製品の概要

    • 製品名

      Anti-C1q antibody [7H8]
      C1q 一次抗体 製品一覧
    • 製品の詳細

      Rat monoclonal [7H8] to C1q
    • 由来種

      Rat
    • 特異性

      Immunization schedule and immunogen used has been described in the following article: Wasiliu, M et al; Monoclonal antibodies to complement components without the need of their prior purification. I. Antibodies to mouse C1q. Hybridoma 1989, 8 (6): 615.

    • アプリケーション

      適用あり: ICC/IF, ELISA, IHC-FoFr, ICC, IHC-Frmore details
    • 種交差性

      交差種: Mouse, Human
    • 免疫原

      Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.

    • ポジティブ・コントロール

      • IHC-Fr: P90 Wild-type mouse.
    • 特記事項

      This product was switched from a hybridoma to a recombinant production format on 25th October 2021.

      This product is a recombinant monoclonal antibody, which offers several advantages including:

      • - High batch-to-batch consistency and reproducibility
      • - Improved sensitivity and specificity
      • - Long-term security of supply
      • - Animal-free production
      For more information see here.

    製品の特性

    • 製品の状態

      Liquid
    • 保存方法

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • バッファー

      Preservative: 0.01% Sodium azide
      Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
    • Concentration information loading...
    • 精製度

      Protein A purified
    • ポリ/モノ

      モノクローナル
    • クローン名

      7H8
    • アイソタイプ

      IgG1
    • 軽鎖の種類

      kappa
    • 研究分野

      • Immunology
      • Innate Immunity
      • Complement
      • Classical Pathway
      • Neuroscience
      • Cell Type Marker
      • Neuron marker
      • Synapse marker
      • Signal Transduction
      • Antibodies
      • c1q
      • Neuroscience
      • Processes

    関連製品

    • Alternative Versions

      • Anti-C1q antibody [7H8] - BSA and Azide free (ab255973)
    • Compatible Secondaries

      • Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
      • Goat Anti-Rat IgG H&L (HRP) (ab205720)
    • Isotype control

      • Rat IgG1, kappa monoclonal [RTK2071] - Isotype control (ab18412)

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab11861の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    ICC/IF (2)
    Use at an assay dependent concentration.
    ELISA (1)
    Use at an assay dependent concentration.
    IHC-FoFr (1)
    Use at an assay dependent concentration.
    ICC (2)
    Use at an assay dependent concentration.
    IHC-Fr
    1/500.
    特記事項
    ICC/IF
    Use at an assay dependent concentration.
    ELISA
    Use at an assay dependent concentration.
    IHC-FoFr
    Use at an assay dependent concentration.
    ICC
    Use at an assay dependent concentration.
    IHC-Fr
    1/500.

    ターゲット情報

    • 機能

      C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.
    • 関連疾患

      Defects in C1QA are a cause of complement component C1q deficiency (C1QD) [MIM:613652]. A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis.
    • 配列類似性

      Contains 1 C1q domain.
      Contains 1 collagen-like domain.
    • 翻訳後修飾

      O-linked glycans consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups.
    • 細胞内局在

      Secreted.
    • Target information above from: UniProt accession P02745 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 参照データベース

      • Entrez Gene: 712 Human
      • Entrez Gene: 713 Human
      • Entrez Gene: 714 Human
      • Entrez Gene: 12259 Mouse
      • Entrez Gene: 12260 Mouse
      • Entrez Gene: 12262 Mouse
      • Omim: 120550 Human
      • Omim: 120570 Human
      • Omim: 120575 Human
      • SwissProt: P02745 Human
      • SwissProt: P02746 Human
      • SwissProt: P02747 Human
      • SwissProt: P14106 Mouse
      • SwissProt: P98086 Mouse
      • SwissProt: Q02105 Mouse
      • Unigene: 632379 Human
      • Unigene: 439957 Mouse
      see all
    • 別名

      • C1qa antibody
      • C1QA_HUMAN antibody
      • C1QB antibody
      • C1QC antibody
      • Complement C1q subcomponent subunit A antibody
      • Complement C1q subcomponent subunit B antibody
      • Complement C1q subcomponent subunit C antibody
      • Complement component 1 q subcomponent alpha polypeptide antibody
      • Complement component C1q A chain antibody
      • Complement component C1q B chain antibody
      • Complement component C1q C chain antibody
      see all

    画像

    • Immunohistochemistry (Frozen sections) - Anti-C1q antibody [7H8] (ab11861)
      Immunohistochemistry (Frozen sections) - Anti-C1q antibody [7H8] (ab11861)This image is kindly provided by Dr. Daniel Wilton from Boston Children’s Hospital.

      Positive staining on P90 Wild-type mouse, and no staining on P90 C1qa Knockout Mouse . Primary antibody was applied at a dilution of 1/500 and anti-VGLUT1 antibody. Secondary antibodies including goat anti rabbit IgG (H+L) highly cross-adsorbed Alexa Fluor 594 and goat anti guinea pig IgG (H+L) highly cross-adsorbed Alexa Fluor 488 were also employed at a dilution of 1/500.

    プロトコール

    • Immunohistochemistry protocols

    Click here to view the general protocols

    データシートおよび資料

    • SDS download

    • Datasheet download

      Download

    参考文献 (12)

    ab11861 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab11861 は 12 報の論文で使用されています。

    • Figueiredo CP  et al. Zika virus replicates in adult human brain tissue and impairs synapses and memory in mice. Nat Commun 10:3890 (2019). PubMed: 31488835
    • Hata J  et al. Complement activation by autoantigen recognition in the growth process of benign prostatic hyperplasia. Sci Rep 9:20357 (2019). PubMed: 31889151
    • Takahashi H  et al. Opposing effects of progranulin deficiency on amyloid and tau pathologies via microglial TYROBP network. Acta Neuropathol 133:785-807 (2017). PubMed: 28070672
    • Bandini S  et al. The non-inflammatory role of C1q during Her2/neu-driven mammary carcinogenesis. Oncoimmunology 5:e1253653 (2016). PubMed: 28123895
    • Zhang Z  et al. Dysregulation of synaptogenesis genes antecedes motor neuron pathology in spinal muscular atrophy. Proc Natl Acad Sci U S A 110:19348-53 (2013). ICC/IF ; Mouse . PubMed: 24191055
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 17 Abreviews or Q&A

    Western blot abreview for Anti-C1q antibody [7H8]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Brain)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    50 µg
    Treatment
    LPS
    Specification
    Brain
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 22°C
    Read More

    DR. Alexandre Magno

    Verified customer

    投稿 Dec 01 2021

    ELISA abreview for Anti-C1q antibody [7H8]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    ELISA
    Sample
    Mouse Tissue sections (Brain)
    Specification
    Brain
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 24°C
    Type
    Sandwich (Capture)
    Read More

    Abcam user community

    Verified customer

    投稿 Jun 07 2021

    Western blot abreview for Anti-C1q antibody [7H8]

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (brain)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    50 µg
    Specification
    brain
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More

    Abcam user community

    Verified customer

    投稿 Jun 03 2021

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-C1q antibody [7H8]

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (brain)
    Antigen retrieval step
    None
    Permeabilization
    Yes - PBST
    Specification
    brain
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 24°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Jun 02 2021

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-C1q antibody [7H8]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate pH 6.0
    Permeabilization
    No
    Specification
    kidney
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Haoran Zha

    Verified customer

    投稿 Mar 14 2016

    Immunocytochemistry/ Immunofluorescence abreview for Anti-C1q [7H8] antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
    Sample
    Human Cell (SHSY5Y)
    Specification
    SHSY5Y
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Apr 28 2014

    Immunocytochemistry abreview for Anti-C1q antibody [7H8]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Mouse Cell (Neuro2a cell line)
    Specification
    Neuro2a cell line
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
    Read More

    Abcam user community

    Verified customer

    投稿 Sep 28 2012

    Immunocytochemistry abreview for Anti-C1q antibody [7H8]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Human Cell (Kelly Neuroblastoma cell line)
    Specification
    Kelly Neuroblastoma cell line
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
    Read More

    Abcam user community

    Verified customer

    投稿 Sep 26 2012

    Question

    Thank you for the quick response. I also sent another email almost immediately after this one yesterday about another one of your antibodies (Anti-C1q antibody [JL-1] (ab71940)). I had asked about endotoxin testing so am I to assume the same for this antibody, that testing hasn’t been done nor is it specifically produced as a low endotoxin product?

    Read More

    Abcam community

    Verified customer

    Asked on Dec 21 2011

    Answer

    I checked ab71940 and you are correct, it is a similar situation. Unless there is a specific note on the product datasheet stating it has low endotoxin levels, then antibodies are not prepared with endotoxin levels in mind and likely have high, albeit unknown, levels. I hope this is helpful. Please contact me again if you have any further questions.

    Read More

    Abcam Scientific Support

    Answered on Dec 21 2011

    Question

    Hello, I have a few questions regarding Anti-C1q antibody [7H8]: does this antibody neutralize C1q and has endotoxin testing been done on this product? I am interested in this antibody for in vivo studies and need to find a low endotoxin batch.

    Read More

    Abcam community

    Verified customer

    Asked on Dec 21 2011

    Answer

    Thank you for your enquiry. ab11861 has not been tested for endotoxin levels. It is not produced specifically as a low endotoxin product. Here is a link to the only reference we know of using this antibody in functional assays: http://www.jleukbio.org/content/85/1/55.full?sid=93020101-044d-4c93-a7ba-70f8391561f9 We are not aware of other studies using clone [7H8] to neutralize C1q. I hope this is helpful. Please contact me again if you have any further questions.

    Read More

    Abcam Scientific Support

    Answered on Dec 21 2011

    1-10 of 17 Abreviews or Q&A

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