製品の概要
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製品名Anti-C1q antibody [7H8]
C1q 一次抗体 製品一覧 -
製品の詳細Rat monoclonal [7H8] to C1q
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由来種Rat
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特異性Immunization schedule and immunogen used has been described in the following article: Wasiliu, M et al; Monoclonal antibodies to complement components without the need of their prior purification. I. Antibodies to mouse C1q. Hybridoma 1989, 8 (6): 615.
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アプリケーション適用あり: ICC, IHC-FoFr, ICC/IF, ELISA, IHC-Frmore details
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種交差性交差種: Mouse, Human
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免疫原
This information is considered to be commercially sensitive
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ポジティブ･コントロール
- C1q can be found on macrophages, follicular dendritic cells, interdigitating cells and further cells of the monocyte-macrophage lineage. These types of cells can be used as positive control.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーPreservative: 0.02% Sodium Azide
Constituents: 0.1% BSA, PBS -
Concentration information loading... -
精製度Protein G purified
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特記事項（精製）0.2 µm filtered
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ポリ/モノモノクローナル
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クローン名7H8
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アイソタイプIgG1
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軽鎖の種類kappa
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab11861 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC | 1/10. | |
| IHC-FoFr | Use at an assay dependent concentration. | |
| ICC/IF | Use at an assay dependent concentration. | |
| ELISA | Use at an assay dependent concentration. | |
| IHC-Fr | 1/10. |
ターゲット情報
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機能C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.
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関連疾患Defects in C1QA are a cause of complement component C1q deficiency (C1QD) [MIM:613652]. A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis.
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配列類似性Contains 1 C1q domain.
Contains 1 collagen-like domain. -
翻訳後修飾O-linked glycans consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups.
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細胞内局在Secreted.
- Information by UniProt
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参照データベース
- Entrez Gene: 712 Human
- Entrez Gene: 713 Human
- Entrez Gene: 714 Human
- Entrez Gene: 12259 Mouse
- Entrez Gene: 12260 Mouse
- Entrez Gene: 12262 Mouse
- Omim: 120550 Human
- Omim: 120570 Human
see all -
別名
- C1qa antibody
- C1QA_HUMAN antibody
- C1QB antibody
see all
画像
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![Immunocytochemistry/ Immunofluorescence - Anti-C1q antibody [7H8] (ab11861)](https://www.abcam.co.jp/ps/products/11/ab11861/Images/ab11861-222846-ab11861iccif.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-C1q antibody [7H8] (ab11861)This image is courtesy of an anonymous Abreviewab11861 staining C1q (green) in human SHSY5Y cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 1% paraformaldehyde and blocked with 10% serum for 20 minutes at room temperature. Samples were incubated with primary antibody (1/50 in PBS) for 2 hours at 24°C. An undiluted Alexa Fluor®488-conjugated goat anti-rat IgG polyclonal was used as the secondary antibody. Counterstained with Hoechst 33258 (blue) for 10 minutes at room temperature.
参考文献
This product has been referenced in:
- Zhang Z et al. Dysregulation of synaptogenesis genes antecedes motor neuron pathology in spinal muscular atrophy. Proc Natl Acad Sci U S A 110:19348-53 (2013). ICC/IF ; Mouse . Read more (PubMed: 24191055) »
- Linnartz B et al. Sialic acid on the neuronal glycocalyx prevents complement C1 binding and complement receptor-3-mediated removal by microglia. J Neurosci 32:946-52 (2012). ICC/IF ; Mouse . Read more (PubMed: 22262892) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"