Anti-C1q 抗体 [7H8] (ab11861)
![Immunohistochemistry (Frozen sections) - Anti-C1q antibody [7H8] (ab11861)](https://www.abcam.co.jp/ps/products/11/ab11861/Images/ab11861-479940-anti-c1q-antibody-7h8-immunohistochemistry-frozen-sections.jpg "Immunohistochemistry (Frozen sections) - Anti-C1q antibody [7H8] (ab11861)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [7H8] to C1q
- Suitable for: ICC/IF, ELISA, IHC-FoFr, ICC, IHC-Fr
- Reacts with: Mouse, Human
製品の概要
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製品名
Anti-C1q antibody [7H8]
C1q 一次抗体 製品一覧 -
製品の詳細
Rat monoclonal [7H8] to C1q -
由来種
Rat -
特異性
Immunization schedule and immunogen used has been described in the following article: Wasiliu, M et al; Monoclonal antibodies to complement components without the need of their prior purification. I. Antibodies to mouse C1q. Hybridoma 1989, 8 (6): 615.
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アプリケーション
適用あり: ICC/IF, ELISA, IHC-FoFr, ICC, IHC-Frmore details -
種交差性
交差種: Mouse, Human -
免疫原
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- IHC-Fr: P90 Wild-type mouse.
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特記事項
This product was switched from a hybridoma to a recombinant production format on 25th October 2021.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
7H8 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab11861の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | (2) |
Use at an assay dependent concentration.
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| ELISA | (1) |
Use at an assay dependent concentration.
|
| IHC-FoFr | (1) |
Use at an assay dependent concentration.
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| ICC | (2) |
Use at an assay dependent concentration.
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| IHC-Fr |
1/500.
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| 特記事項 |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
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ELISA
Use at an assay dependent concentration. |
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IHC-FoFr
Use at an assay dependent concentration. |
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ICC
Use at an assay dependent concentration. |
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IHC-Fr
1/500. |
ターゲット情報
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機能
C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes. -
関連疾患
Defects in C1QA are a cause of complement component C1q deficiency (C1QD) [MIM:613652]. A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis. -
配列類似性
Contains 1 C1q domain.
Contains 1 collagen-like domain. -
翻訳後修飾
O-linked glycans consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 712 Human
- Entrez Gene: 713 Human
- Entrez Gene: 714 Human
- Entrez Gene: 12259 Mouse
- Entrez Gene: 12260 Mouse
- Entrez Gene: 12262 Mouse
- Omim: 120550 Human
- Omim: 120570 Human
see all -
別名
- C1qa antibody
- C1QA_HUMAN antibody
- C1QB antibody
see all
画像
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Immunohistochemistry (Frozen sections) - Anti-C1q antibody [7H8] (ab11861)This image is kindly provided by Dr. Daniel Wilton from Boston Children’s Hospital.
Positive staining on P90 Wild-type mouse, and no staining on P90 C1qa Knockout Mouse . Primary antibody was applied at a dilution of 1/500 and anti-VGLUT1 antibody. Secondary antibodies including goat anti rabbit IgG (H+L) highly cross-adsorbed Alexa Fluor 594 and goat anti guinea pig IgG (H+L) highly cross-adsorbed Alexa Fluor 488 were also employed at a dilution of 1/500.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (12)
ab11861 は 12 報の論文で使用されています。
- Figueiredo CP et al. Zika virus replicates in adult human brain tissue and impairs synapses and memory in mice. Nat Commun 10:3890 (2019). PubMed: 31488835
- Hata J et al. Complement activation by autoantigen recognition in the growth process of benign prostatic hyperplasia. Sci Rep 9:20357 (2019). PubMed: 31889151
- Takahashi H et al. Opposing effects of progranulin deficiency on amyloid and tau pathologies via microglial TYROBP network. Acta Neuropathol 133:785-807 (2017). PubMed: 28070672
- Bandini S et al. The non-inflammatory role of C1q during Her2/neu-driven mammary carcinogenesis. Oncoimmunology 5:e1253653 (2016). PubMed: 28123895
- Zhang Z et al. Dysregulation of synaptogenesis genes antecedes motor neuron pathology in spinal muscular atrophy. Proc Natl Acad Sci U S A 110:19348-53 (2013). ICC/IF ; Mouse . PubMed: 24191055