Anti-SMG9 抗体 (ab85659)
Key features and details
- Rabbit polyclonal to SMG9
- Suitable for: IP, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-SMG9 antibody -
製品の詳細
Rabbit polyclonal to SMG9 -
由来種
Rabbit -
アプリケーション
適用あり: IP, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant -
免疫原
Synthetic peptide corresponding to Human SMG9.
Database link: NP_061981.2 -
ポジティブ・コントロール
- HeLa cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab85659の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IP |
Use at 5-10 µg/mg of lysate.
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IHC-P |
1/2000 - 1/10000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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IP
Use at 5-10 µg/mg of lysate. |
IHC-P
1/2000 - 1/10000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Is recruited by release factors to stalled ribosomes together with SMG1 and SMG8 (forming the SMG1C protein kinase complex) and, in the SMG1C complex, is required for the efficient association between SMG1 and SMG8. -
配列類似性
Belongs to the SMG9 family. -
翻訳後修飾
Phosphorylated by SMG1. - Information by UniProt
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参照データベース
- Entrez Gene: 56006 Human
- Entrez Gene: 71997 Mouse
- Entrez Gene: 365215 Rat
- Omim: 613176 Human
- SwissProt: Q9H0W8 Human
- SwissProt: Q9DB90 Mouse
- SwissProt: Q5PQS6 Rat
- Unigene: 466875 Human
see all -
別名
- C19orf61 antibody
- Chromosome 19 open reading frame 61 antibody
- DKFZp564H1322 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling SMG9 with ab85689 at 1/5000 (0.2µg/ml). Detection: DAB.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab85659 は 1 報の論文で使用されています。
- Huang L et al. Antisense suppression of the nonsense mediated decay factor Upf3b as a potential treatment for diseases caused by nonsense mutations. Genome Biol 19:4 (2018). PubMed: 29334995