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  1. Link

    c-reactive-protein-antibody-c6-ab8278.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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Anti-C Reactive Protein 抗体 [C6] (ab8278)

  • Datasheet
  • SDS
Submit a review Q&A (12)References (3)

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Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Sandwich ELISA - Anti-C Reactive Protein antibody [C6] (ab8278)
  • Sandwich ELISA - Anti-C Reactive Protein antibody [C6] (ab8278)

Key features and details

  • Mouse monoclonal [C6] to C Reactive Protein
  • Suitable for: Sandwich ELISA
  • Reacts with: Human
  • Isotype: IgG2a

Conjugates logo Related conjugates and formulations

HRP

こちらの製品もご検討ください

タンパク質
Product image
Recombinant Human C Reactive Protein (ab167710)
一次抗体
HRP Anti-C Reactive Protein antibody (ab19175)
抗体ペア
Product image
Human CRP Antibody Pair - BSA and Azide free (ab270347)

関連製品

製品の概要

  • 製品名

    Anti-C Reactive Protein antibody [C6]
    C Reactive Protein 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [C6] to C Reactive Protein
  • 由来種

    Mouse
  • アプリケーション

    適用あり: Sandwich ELISAmore details
  • 種交差性

    交差種: Human
  • 免疫原

    Full length native protein (purified) corresponding to Human C Reactive Protein.
    Database link: P02741

  • 特記事項

    Specific antibody titer in ELISA is > 10 to the power of -6

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー

    pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 精製度

    Protein A purified
  • ポリ/モノ

    モノクローナル
  • クローン名

    C6
  • ミエローマ

    Sp2/0
  • アイソタイプ

    IgG2a
  • 軽鎖の種類

    unknown
  • 研究分野

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Cardiovascular
    • Blood
    • Acute Phase Reactants
    • Immunology
    • Innate Immunity
    • Complement
    • Classical Pathway
    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators
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    • Apoptosis
    • Metabolism
    • Types of disease
    • Metabolic disorders
    • Neuroscience
    • Processes

関連製品

  • Alternative Versions

    • HRP Anti-C Reactive Protein antibody [C6] (ab24462)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Conjugation kits

    • Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link® (ab201795)
  • Isotype control

    • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
  • Recombinant Protein

    • Recombinant Human C Reactive Protein (ab167710)
  • sELISA pair antibody

    • Anti-C Reactive Protein antibody [C2] (ab136176)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab8278の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [C2] to C Reactive Protein (ab136176). Recommended pairs for sandwich immunoassay
(capture - detection):
C2 - C6
C5 - C6
C7 - C6.
特記事項
Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [C2] to C Reactive Protein (ab136176). Recommended pairs for sandwich immunoassay
(capture - detection):
C2 - C6
C5 - C6
C7 - C6.

ターゲット情報

  • 機能

    Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
  • 組織特異性

    Found in plasma.
  • 配列類似性

    Belongs to the pentaxin family.
    Contains 1 pentaxin domain.
  • 細胞内局在

    Secreted.
  • Target information above from: UniProt accession P02741 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 1401 Human
    • Omim: 123260 Human
    • SwissProt: P02741 Human
    • Unigene: 709456 Human
    • Unigene: 76452 Human
    • 別名

      • Pentraxin 1, short antibody
      • C reactive protein antibody
      • C reactive protein pentraxin related antibody
      • C-reactive protein(1-205) antibody
      • CRP antibody
      • CRP_HUMAN antibody
      • MGC88244 antibody
      • Pentraxin 1 antibody
      • PTX 1 antibody
      • PTX1 antibody
      see all

    画像

    • Sandwich ELISA - Anti-C Reactive Protein antibody [C6] (ab8278)
      Sandwich ELISA - Anti-C Reactive Protein antibody [C6] (ab8278)

      Calibration curve using ab8277 [C2] (biotinylated) and ab8278 [C6] (labelled with stable Eu3+ chelate) in a sandwich ELISA. Mixture of antibodies and antigen (10 µl sample) were incubated for 10 minutes at R.T. in streptavidin coated plates.

    • Sandwich ELISA - Anti-C Reactive Protein antibody [C6] (ab8278)
      Sandwich ELISA - Anti-C Reactive Protein antibody [C6] (ab8278)
      Sensitivity to EDTA. Serum samples with (dark blue) and w/o (light blue) Ca2+ ions were tested by two two-site combinations of anti-CRP MAbs. Ab10028 [C4] is sensitive to the conformational changes of CRP molecule and thus the assay gives no signal in the presence of EDTA. Sandwich immunoassay utilizing ab8277 [C2] and ab8278 [C6] recognizes the antigen with the same efficiency in the presence and in the absence of EDTA.

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    データシートおよび資料

    • SDS download

    • Datasheet download

      Download

    参考文献 (3)

    ab8278 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab8278 は 3 報の論文で使用されています。

    • Ouyang M & Di Carlo D Nanoplasmonic swarm biosensing using single nanoparticle colorimetry. Biosens Bioelectron 132:162-170 (2019). PubMed: 30875628
    • Zong C  et al. Metal-enhanced chemiluminescence detection of C-reaction protein based on silver nanoparticle hybrid probes. Talanta 199:164-169 (2019). PubMed: 30952241
    • Cheah IK  et al. Administration of Pure Ergothioneine to Healthy Human Subjects: Uptake, Metabolism, and Effects on Biomarkers of Oxidative Damage and Inflammation. Antioxid Redox Signal 26:193-206 (2017). PubMed: 27488221

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 12 Abreviews or Q&A

    Question

    Thank you for your reply. I just wonder if only one antibody binds to one CRP molecule or five antibody binds to one CRP molecule since the CRP molecule is a pentamer containing five identical monomers. Or isthis informationalso confidential?

    Thank you again

    Best wishes

    Read More

    Abcam community

    Verified customer

    Asked on Oct 08 2012

    Answer

    Thank you for contacting us.

    I am sorry we do not have any data to confirm how many ab molecules binds to CRP. Theoretically, I agree there is possibility of binding 5 ab molecule per pentamer of CRP however we can't be sure as we do not know for how many chains the epitope is exposed for antibody binding.

    I am sorry due to non availability of tested we will not be able to confirm this.

    I hope this information is nevertheless helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Oct 08 2012

    Question

    Sorry that I did not include the product codes in the previous email. The product codes for anti-CRP C2 and C6 are ab24461 and ab8278, respectively.

    Thank you

    Read More

    Abcam community

    Verified customer

    Asked on Oct 05 2012

    Answer

    Thank you for contacting us. Dr. Tanya is out of office today so I am dealing with her inquiries.

    The immunogen for these antibodies is a proprietary information. These antibodies are raised sing full length human CRP protein. The two products are tested positive in sandwich ELISA.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Oct 05 2012

    Question


    Thank you for updating this. My previous order ***.

    Would you please ship it after Aug. 20? I'll not be available next week. I prefer to get it when I'm in the lab.

    Thanks,

    Read More

    Abcam community

    Verified customer

    Asked on Aug 10 2012

    Answer

    Thank you very much for your reply.

    It will be no problem to ship it after August 20. We are actually expecting to have this antibody in stock by August 27, so it should arrive shortly after that date. The antibody will be arriving free of charge on the order ***.

    So that we can look into the trouble with these antibodies, could you also briefly describe the protocol used with ab8278 and ab8279 in sandwich ELISA?

    I look forward to hearing from you. Thank you very much and have a nice day!

    Read More

    Abcam Scientific Support

    Answered on Aug 10 2012

    Question

    High background using C5 (capture) and C6 (Cy5.5 conjugated detection) antibody in sELISA. Plasma samples and recombinant standards.

    Read More

    Abcam community

    Verified customer

    Asked on Aug 10 2012

    Answer

    Thank you again for letting us know about the trouble with the CRP antibodies in ELISA.

    We now have the unconjugated C2 clone in our catalog as ab136176, and I'd be happy to send a free of charge vial for your to try.

    https://www.abcam.com/C-Reactive-Protein-antibody-C2-ab136176.html

    Could you please let me know your original order or PO number and I will send this out promptly?

    Thank you for your patience in resolving this issue, and please let me know if there is anything else that we can do for you.

    Read More

    Abcam Scientific Support

    Answered on Aug 10 2012

    Question

    Thank you for sharing this with me. It seems that the capturing antibody is biotinylated. That could explain the non-specific issue. It would be nice to get the un-conjugated C2 antibody to test.

    Do you have the protocol for testing blood plasma CRP protein? How much dilution for human blood plasma?

    Is DELFIA washing buffer specially designed for the PerkinElmer instrument? Or it is good for general use to minimized non-specific binding?

    Many thanks,

    Read More

    Abcam community

    Verified customer

    Asked on Jul 26 2012

    Answer

    Thank you very much for your reply.

    I agree that the differences in the protocol may be contributing to the background you're experiencing. I'm working with my colleagues to see if we can provide the unconjugated C2, and I will update you as soon as I have more information about this antibody.

    We do not have a full protocol for testing blood plasma CRP protein, and since the titer of this protein is variable, it would be a good idea to try a few dilutions of your plasma samples to determine the optimal dilution. I would recommend using at least a 1:1 dilution to reduce any possible matrix effects that can obscure the specific signal.

    The DELFIA washing buffer is only necessary for the specific one-step immunoassay that the lab ran with the Eu-conjugated detection antibody, and I don't think that this buffer will improve the results in a more standard ELISA. PBS or PBST should be a sufficient washing buffer to reduce non-specific binding.

    I will be back in touch with you once I've heard from my colleagues about the C2 clone. Please let me know if you have any questions in the meantime. Thank you for your patience!

    Read More

    Abcam Scientific Support

    Answered on Jul 26 2012

    Question

    High background using C5 (capture) and C6 (Cy5.5 conjugated detection) antibody in sELISA. Plasma samples and recombinant standards.

    Read More

    Abcam community

    Verified customer

    Asked on Jul 25 2012

    Answer

    Thank you very much for your call earlier this week and for letting us know about the trouble with these antibodies.

    I've heard back from the lab, and these antibodies were actually tested together in a one-step fluoroimmunoassay;please see the full protocol attached. This protocol is not a standard sandwich ELISA protocol, and I'm not sure that it will be helpful as a referencefor the kind of assay that you are running.

    Based on the information that you've given me, I'd be happy to send a different antibody to try in the assay. We only have the HRP-conjugated C2 clone in our catalog at this time, but I cancheck whetherweare able tosend a vial of unconjugated C2 if you'd like. If there is a different CRP antibody that you would like to try, I can send that instead.

    I look forward to hearing from you. Please let me know if there is an antibody or pair of antibodies that you would like to try, or if you have any questions about this information.

    Read More

    Abcam Scientific Support

    Answered on Jul 25 2012

    Question

    We are currently looking for a good pairing for C-Reactive protein antibodies and wonder if you guys could recommend any pair of antibodies? The antibodies will be used in sandwich ELISA and we are looking for antibodies which are already paired by Abcam for optimal binding and lowest cross reactivity of capture and detection antibody.
    We've noticed that it is recommended to pair antibodies according to their C number, for example C2-C6, C5-C6 and C7-C6, information found on website: https://www.abcam.com/C-Reactive-Protein-antibody-C6-ab8278.html.
    It is best to pair antibodies to their C numbers as mentioned?

    Looking forward to your reply.


    Thanks and regards,

    Read More

    Abcam community

    Verified customer

    Asked on Apr 23 2012

    Answer

    Thank you for contacting us.
    We do not use the terminology C number. These does not depicts any information other the clone numbers for a particular antibody.
    The sELISA pairs we recommend are
    The best pair is C2-C6 (ab24461 detection - ab8278 capture)
    C5-C6 (ab8279 capture - ab24462 detection)
    C7-C6 (ab8280 capture - ab24462 detection)
    Please ab24462 is a HRP conjugated anti C reactive protein antibody; due to the conjugation it will be a good detection antibody.
    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Apr 23 2012

    Question

    Hello,   We recently purchased and tested the 500 ug (2mg/ml) ab8278 and were wondering whether or not you sell it in a more concentrated amount/larger lyophilized amount at 2mg/ml.   Thank you.  

    Read More

    Abcam community

    Verified customer

    Asked on Dec 08 2011

    Answer

    Thank you for contacting Abcam. If you wish to place a bulk order I can happily forward your request to in house specialist how deals with these matters. However we generally only do custom batches for larger orders. If you just want to increase the concentration of the antibody, then I would recommend using one of our concentration kits: https://www.abcam.com/Antibody-Concentration-Kit-3-columns-ab102778.html Please let me know if you would like me to pass your information onto our bulk specialist. If there is anything else I can help you with do not hesit

    Read More

    Abcam Scientific Support

    Answered on Dec 08 2011

    Question

    What concentrations of capture and detection antibodies were used to generate the curve on your ab8278 datasheet?

    Read More

    Abcam community

    Verified customer

    Asked on Mar 28 2007

    Answer

    Thank you for your enquiry. For the curve on our datasheet, the following amounts of antibody were used: C2-biotinylated: 100 ng/well (100 ul) C6-Eu (Europium) labeled: 200 ng/well (50 ul) Please do note that the amounts and concentrations depend on the application, so this information should be considered only as starting point suggestions for the optimization.

    Read More

    Abcam Scientific Support

    Answered on Mar 28 2007

    Question

    Which form of the protein was used as the immunogen for this antibody: monomer or pentamer?

    Read More

    Abcam community

    Verified customer

    Asked on Oct 12 2006

    Answer

    Thank you for your enquiry. I can confirm that the antigen for anti-CRP ab8278 is the pentamer form. Please do not hesitate to contact us if you have additional questions.

    Read More

    Abcam Scientific Support

    Answered on Oct 13 2006

    1-10 of 12 Abreviews or Q&A

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