Anti-c-Jun (phospho T91 + T93) 抗体 [C-J 4C4/1] - BSA and Azide free (ab270279)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun (phospho T91 + T93) antibody [C-J 4C4/1] - BSA and Azide free (ab270279)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun (phospho T91 + T93) antibody [C-J 4C4/1] - BSA and Azide free (ab270279)

Key features and details

  • Mouse monoclonal [C-J 4C4/1] to c-Jun (phospho T91 + T93) - BSA and Azide free
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-c-Jun (phospho T91 + T93) antibody [C-J 4C4/1] - BSA and Azide free
    c-Jun 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [C-J 4C4/1] to c-Jun (phospho T91 + T93) - BSA and Azide free

  • 由来種

    Mouse

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    See all applications and species data

  • 免疫原

    Synthetic peptide corresponding to Human c-Jun (phospho T91 + T93).
    Database link: P05412

  • ポジティブ･コントロール

    • IHC-P: Human cervical tumor.

  • 特記事項

    ab270279 is a carrier free version of ab270242.

     

製品の特性

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab270279 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
IHC-P
Human
アプリケーション Abreviews 特記事項
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
特記事項
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306).

  • 配列類似性

    Belongs to the bZIP family. Jun subfamily.
    Contains 1 bZIP (basic-leucine zipper) domain.

  • 翻訳後修飾

    Ubiquitinated by the SCF(FBXW7), leading to its degradation. Ubiquitination takes place following phosphorylation, that promotes interaction with FBXW7.
    Phosphorylated by CaMK4 and PRKDC; phosphorylation enhances the transcriptional activity. Phosphorylated by HIPK3. Phosphorylated by DYRK2 at Ser-243; this primes the protein for subsequent phosphorylation by GSK3B at Thr-239. Phosphorylated at Thr-239, Ser-243 and Ser-249 by GSK3B; phosphorylation reduces its ability to bind DNA. Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-286 thereby promoting JUN-mediated cell proliferation and transformation. Phosphorylated by PLK3 following hypoxia or UV irradiation, leading to increase DNA-binding activity.
    Acetylated at Lys-271 by EP300.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Activator protein 1 antibody
      • AP 1 antibody
      • AP-1 antibody
      • AP1 antibody
      • cJun antibody
      • Enhancer Binding Protein AP1 antibody
      • Jun Activation Domain Binding Protein antibody
      • JUN antibody
      • Jun oncogene antibody
      • JUN protein antibody
      • Jun proto oncogene antibody
      • JUN_HUMAN antibody
      • JUNC antibody
      • Oncogene JUN antibody
      • p39 antibody
      • Proto oncogene c jun antibody
      • Proto oncogene cJun antibody
      • Proto-oncogene c-jun antibody
      • Transcription Factor AP 1 antibody
      • Transcription factor AP-1 antibody
      • Transcription Factor AP1 antibody
      • V jun avian sarcoma virus 17 oncogene homolog antibody
      • V jun sarcoma virus 17 oncogene homolog (avian) antibody
      • V jun sarcoma virus 17 oncogene homolog antibody
      • V-jun avian sarcoma virus 17 oncogene homolog antibody
      • vJun Avian Sarcoma Virus 17 Oncogene Homolog antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun (phospho T91 + T93) antibody [C-J 4C4/1] - BSA and Azide free (ab270279)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun (phospho T91 + T93) antibody [C-J 4C4/1] - BSA and Azide free (ab270279)

      Formalin-fixed, paraffin-embedded human cervical tumour stained for c-Jun (phospho T91 + T93) using ab270242 at 2 µg/ml in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab270242).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun (phospho T91 + T93) antibody [C-J 4C4/1] - BSA and Azide free (ab270279)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun (phospho T91 + T93) antibody [C-J 4C4/1] - BSA and Azide free (ab270279)

      Formalin-fixed, paraffin-embedded human cervical tumour stained for c-Jun (phospho T91 + T93) using ab270242 at 2 µg/ml in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab270242).

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab270279 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab270279 は論文での使用が確認できていません。

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