Anti-c-Jun 抗体 (ab5795)

Reviews (1)Q&A (2)

Key features and details

  • Rabbit polyclonal to c-Jun
  • Suitable for: WB
  • Reacts with: Mouse
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-c-Jun antibody
    c-Jun 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to c-Jun

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WBmore details

  • 種交差性

    交差種: Mouse
    交差が予測される動物種: Rat, Sheep, Chicken, Cow, Human, Pig, Xenopus laevis

  • 免疫原

    Synthetic peptide corresponding to Mouse c-Jun aa 91-105.
    Sequence:

    TPTPTQFLCPKNVTD

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • NIH 3T3 cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab5795 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 10 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 39.7 kDa).

ターゲット情報

  • 機能

    Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306).

  • 配列類似性

    Belongs to the bZIP family. Jun subfamily.
    Contains 1 bZIP (basic-leucine zipper) domain.

  • 翻訳後修飾

    Ubiquitinated by the SCF(FBXW7), leading to its degradation. Ubiquitination takes place following phosphorylation, that promotes interaction with FBXW7.
    Phosphorylated by CaMK4 and PRKDC; phosphorylation enhances the transcriptional activity. Phosphorylated by HIPK3. Phosphorylated by DYRK2 at Ser-243; this primes the protein for subsequent phosphorylation by GSK3B at Thr-239. Phosphorylated at Thr-239, Ser-243 and Ser-249 by GSK3B; phosphorylation reduces its ability to bind DNA. Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-286 thereby promoting JUN-mediated cell proliferation and transformation. Phosphorylated by PLK3 following hypoxia or UV irradiation, leading to increase DNA-binding activity.
    Acetylated at Lys-271 by EP300.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • Activator protein 1 antibody
    • AP 1 antibody
    • AP-1 antibody
    • AP1 antibody
    • cJun antibody
    • Enhancer Binding Protein AP1 antibody
    • Jun Activation Domain Binding Protein antibody
    • JUN antibody
    • Jun oncogene antibody
    • JUN protein antibody
    • Jun proto oncogene antibody
    • JUN_HUMAN antibody
    • JUNC antibody
    • Oncogene JUN antibody
    • p39 antibody
    • Proto oncogene c jun antibody
    • Proto oncogene cJun antibody
    • Proto-oncogene c-jun antibody
    • Transcription Factor AP 1 antibody
    • Transcription factor AP-1 antibody
    • Transcription Factor AP1 antibody
    • V jun avian sarcoma virus 17 oncogene homolog antibody
    • V jun sarcoma virus 17 oncogene homolog (avian) antibody
    • V jun sarcoma virus 17 oncogene homolog antibody
    • V-jun avian sarcoma virus 17 oncogene homolog antibody
    • vJun Avian Sarcoma Virus 17 Oncogene Homolog antibody
    see all

参考文献 (0)

ab5795 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab5795 は論文での使用が確認できていません。

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ChIP abreview for Anti-c-Jun antibody

 Average
Abreviews
Application
ChIP
Sample
Mouse Tissue lysate - whole (skin epidermis)
Specification
skin epidermis
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Detection step
Semiquantitative PCR
Positive control
Untreated and TPA treated samples were used. Only in the case of TPA treated sample, a specific signal was obtained. Another c-jun antibody from a different company, which we had tested earlier and obtained positive result, was also included in parallel. In the case of both Abcam and the other companies antibodies, specific signal was obtained only when the skin was treated with TPA. However, the other companies antibody was more efficient in immunoprecipitation in comparison to Abcam antibody.
Negative control
A region corresponding to the coding sequence of the gene, which does not reveal any c-jun binding site by bioinformatics analysis, was used for PCR amplification. However, no amplification was observed.
Also there was no binding in the absence of TPA as mentioned above.
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Dr. milan surjit

Verified customer

投稿 Sep 21 2007

Question

We recently purchased the above antibody (ab5795; anti-cJun) and are using it in Western blots. We have two specific questions: (1) Does it cross react with other members of the Jun family (eg. JunD, JunB)? (2) Does it pick up both phosphorylated and non-phosphorylated forms of cJun?

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Answer

Below is the information that we have for ab5795: Ab5795 detects cJun from mouse cells. Using the sequence information (TPTPTQFLCPKNVTD) you can run a BLAST search on Genbank to determine if cross-reactivity would take place. The originator has not tested ab5795 for cross-reactivity with other members of the Jun family. The immunogen used was not phosphorylated; therefore, ab5795 will only detect the non-phosphorylated protein.

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Question

The NIH 3T3 serum stimulated cells that were used - what is the protocol that was used to stimulate the cells?

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Answer

Our material/methods says that it was 20% serum stimulated for 2hrs at physiological temperature. If you have any more questions, please do let us know.

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