Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
リコンビナントRabMAb

Anti-BubR1 抗体 [EPR22544-48] (ab254326)

Submit a review Submit a question References (2)
Western blot - Anti-BubR1 antibody [EPR22544-48] (ab254326)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BubR1 antibody [EPR22544-48] (ab254326)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BubR1 antibody [EPR22544-48] (ab254326)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BubR1 antibody [EPR22544-48] (ab254326)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BubR1 antibody [EPR22544-48] (ab254326)
  • Immunocytochemistry/ Immunofluorescence - Anti-BubR1 antibody [EPR22544-48] (ab254326)
  • Immunocytochemistry/ Immunofluorescence - Anti-BubR1 antibody [EPR22544-48] (ab254326)
  • Immunocytochemistry/ Immunofluorescence - Anti-BubR1 antibody [EPR22544-48] (ab254326)
  • Anti-BubR1 antibody [EPR22544-48] (ab254326)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22544-48] to BubR1
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-BubR1 antibody [EPR22544-48]
    BubR1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR22544-48] to BubR1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-P, ICC/IFmore details
    適用なし: Flow Cyt,IHC-Fr or IP

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HeLa and Raji cell lysate; Mouse testis, thymus and rat testis tissue lysate. IHC-P: Human breast cancer and testis tissue; Mouse and rat testis tissue. ICC/IF: SK-OV-3 and HeLa cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab254326の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000. Predicted molecular weight: 119 kDa.
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/50.
特記事項
WB
1/1000. Predicted molecular weight: 119 kDa.
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/50.
追加情報
Is unsuitable for Flow Cyt,IHC-Fr or IP.

ターゲット情報

  • 機能

    Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression.

  • 組織特異性

    Highly expressed in thymus followed by spleen. Preferentially expressed in tissues with a high mitotic index.

  • 関連疾患

    Note=Defects in BUB1B are associated with tumor formation.
    Defects in BUB1B are the cause of premature chromatid separation trait (PCS) [MIM:176430]. PCS consists of separate and splayed chromatids with discernible centromeres and involves all or most chromosomes of a metaphase. It is found in up to 2% of metaphases in cultured lymphocytes from approximately 40% of normal individuals. When PCS is present in 5% or more of cells, it is known as the heterozygous PCS trait and has no obvious phenotypic effect, although some have reported decreased fertility. Inheritance is autosomal dominant.
    Defects in BUB1B are the cause of mosaic variegated aneuploidy syndrome (MVA) [MIM:257300]. MVA is a severe autosomal recessive developmental disorder characterized by mosaic aneuploidies, predominantly trisomies and monosomies, involving multiple different chromosomes and tissues. The proportion of aneuploid cells varies but is usually more than 25% and is substantially greater than in normal individuals. Affected individuals typically present with severe intrauterine growth retardation and microcephaly. Eye anomalies, mild dysmorphism, variable developmental delay, and a broad spectrum of additional congenital abnormalities and medical conditions may also occur. The risk of malignancy is high, with rhabdomyosarcoma, Wilms tumor and leukemia reported in several cases. MVA is caused by biallelic mutations in the BUB1B gene.

  • 配列類似性

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. BUB1 subfamily.
    Contains 1 BUB1 N-terminal domain.
    Contains 1 protein kinase domain.

  • ドメイン

    The D-box targets the protein for rapid degradation by ubiquitin-dependent proteolysis during the transition from mitosis to interphase.
    The BUB1 N-terminal domain directs kinetochore localization and binding to BUB3.

  • 翻訳後修飾

    Proteolytically cleaved by caspase-3 in a cell cycle specific manner. The cleavage might be involved in the durability of the cell cycle delay. Caspase-3 cleavage is associated with abrogation of the mitotic checkpoint. The major site of cleavage is at Asp-610.
    Acetylation at Lys-250 regulates its degradation and timing in anaphase entry.
    Ubiquitinated. Degradated by the proteasome.
    Sumoylated by SUMO2 and SUMO3. The sumoylation mediates the association with CENPE at the kinetochore.
    Autophosphorylated in vitro. Intramolecular autophosphorylation is stimulated by CENPE. Phosphorylated during mitosis and hyperphosphorylated in mitotically arrested cells. Phosphorylation at Ser-670 and Ser-1043 occurs at kinetochores upon mitotic entry with dephosphorylation at the onset of anaphase.

  • 細胞内局在

    Cytoplasm. Nucleus. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > centrosome. Cytoplasmic in interphase cells. Associates with the kinetochores in early prophase. Kinetochore localization requires BUB1, PLK1 and CASC5.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Beta homolg of S. cerevisiae BUB 1 antibody
      • Beta homolg of S. cerevisiae budding uninhibited by benzimidazoles antibody
      • BUB 1B antibody
      • BUB1 budding uninhibited by benzimidazoles 1 homolog beta antibody
      • Bub1A antibody
      • BUB1B antibody
      • BUB1B_HUMAN antibody
      • BUB1beta antibody
      • BUBR1 antibody
      • Budding Uninhibited by Benzimidazoles 1 beta antibody
      • Budding uninhibited by benzimidazoles 1 homolog beta (yeast) antibody
      • hBUBR1 antibody
      • MAD3/BUB1 related protein kinase antibody
      • MAD3/BUB1-related protein kinase antibody
      • MAD3L antibody
      • Mitotic checkpoint gene BUB1B antibody
      • Mitotic checkpoint kinase MAD3L antibody
      • Mitotic checkpoint serine/threonine protein kinase BUB1 beta antibody
      • Mitotic checkpoint serine/threonine-protein kinase BUB1 beta antibody
      • MVA1 antibody
      • OTTHUMP00000160319 antibody
      • Protein SSK1 antibody
      • SSK 1 antibody
      • SSK1 antibody
      see all

    画像

    • Western blot - Anti-BubR1 antibody [EPR22544-48] (ab254326)
      Western blot - Anti-BubR1 antibody [EPR22544-48] (ab254326)
      All lanes : Anti-BubR1 antibody [EPR22544-48] (ab254326) at 1/1000 dilution

      Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
      Lane 2 : Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate
      Lane 3 : Mouse testis tissue lysate
      Lane 4 : Mouse thymus tissue lysate
      Lane 5 : Rat testis tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 119 kDa



      Blocking/dilution buffer and concentration: 5% NFDM/TBST

      The molecular weight observed is consistent with what has been described in the literature (PMID: 26889953).

      Exposure time:

      Lanes 1-2: 70 seconds

      Lane 3: 48 seconds

      Lanes 4-5: 3 minutes 

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BubR1 antibody [EPR22544-48] (ab254326)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BubR1 antibody [EPR22544-48] (ab254326)

      Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling BubR1 with ab254326 at 1/1000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat testis (PMID: 20411023). The section was incubated with ab254326 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). 

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BubR1 antibody [EPR22544-48] (ab254326)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BubR1 antibody [EPR22544-48] (ab254326)

      Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling BubR1 with ab254326 at 1/1000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse testis (PMID: 20411023). The section was incubated with ab254326 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). 

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BubR1 antibody [EPR22544-48] (ab254326)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BubR1 antibody [EPR22544-48] (ab254326)

      Immunohistochemical analysis of paraffin-embedded human testis tissue labeling BubR1 with ab254326 at 1/1000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human testis (PMID: 20411023). The section was incubated with ab254326 for 30 mins at room temperature. Counterstained with hematoxylin. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). 

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BubR1 antibody [EPR22544-48] (ab254326)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BubR1 antibody [EPR22544-48] (ab254326)

      Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling BubR1 with ab254326 at 1/1000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human breast cancer is observed. The section was incubated with ab254326 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). 

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-BubR1 antibody [EPR22544-48] (ab254326)
      Immunocytochemistry/ Immunofluorescence - Anti-BubR1 antibody [EPR22544-48] (ab254326)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SK-OV-3 (human ovarian cancer epithelial cell) cells labeling BubR1 with ab254326 at 1/50 dilution, followed by a AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution (Green). Confocal image showing specific staining on centromeres of SK-OV-3 cell line in M phase. The nuclear counterstain is DAPI (Blue). Tubulin was stained using Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is an AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution 

    • Immunocytochemistry/ Immunofluorescence - Anti-BubR1 antibody [EPR22544-48] (ab254326)
      Immunocytochemistry/ Immunofluorescence - Anti-BubR1 antibody [EPR22544-48] (ab254326)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labeling BubR1 with ab254326 at 1/50 dilution, followed by a AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution (Green).Confocal image showing cytoplasmic staining in HeLa cell line. The nuclear counterstain is DAPI (Blue). Tubulin was stained using Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is an AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution 

    • Immunocytochemistry/ Immunofluorescence - Anti-BubR1 antibody [EPR22544-48] (ab254326)
      Immunocytochemistry/ Immunofluorescence - Anti-BubR1 antibody [EPR22544-48] (ab254326)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labeling BubR1 with ab254326 at 1/50 dilution, followed by a AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution (Green). Confocal image showing specific staining on centromeres of HeLa cell line in M phase. The nuclear counterstain is DAPI (Blue). Tubulin was stained using Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is an AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution 

    • Anti-BubR1 antibody [EPR22544-48] (ab254326)
      Anti-BubR1 antibody [EPR22544-48] (ab254326)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (2)

    ab254326 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab254326 は 2 報の論文で使用されています。

    • Ding ZM  et al. Diethylstilbestrol exposure disrupts mouse oocyte meiotic maturation in vitro through affecting spindle assembly and chromosome alignment. Chemosphere 249:126182 (2020). PubMed: 32078850
    • Zhang SX  et al. Bisphenol B Exposure Disrupts Mouse Oocyte Meiotic Maturation in vitro Through Affecting Spindle Assembly and Chromosome Alignment. Front Cell Dev Biol 8:616771 (2020). PubMed: 33392205

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    There are currently no Customer reviews or Questions for ab254326.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com