Anti-Bub1 抗体 (ab9000)
Key features and details
- Rabbit polyclonal to Bub1
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Bub1 antibody
Bub1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Bub1 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Human -
免疫原
Fusion protein (His-tag) corresponding to Human Bub1 aa 1-312 (N terminal).
Database link: O43683
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide -
Concentration information loading... -
精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab9000 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | Use at an assay dependent dilution. | |
| WB | 1/2000. Predicted molecular weight: 122 kDa. |
ターゲット情報
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機能
Serine/threonine-protein kinase that performs 2 crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Has a key role in the assembly of checkpoint proteins at the kinetochore, being required for the subsequent localization of CENPF, BUB1B, CENPE and MAD2L1. Required for the kinetochore localization of PLK1. Plays an important role in defining SGOL1 localization and thereby affects sister chromatid cohesion. Acts as a substrate for anaphase-promoting complex or cyclosome (APC/C) in complex with its activator CDH1 (APC/C-Cdh1). Necessary for ensuring proper chromosome segregation and binding to BUB3 is essential for this function. Can regulate chromosome segregation in a kinetochore-independent manner. Can phosphorylate BUB3. The BUB1-BUB3 complex plays a role in the inhibition of APC/C when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. Kinase activity is essential for inhibition of APC/CCDC20 and for chromosome alignment but does not play a major role in the spindle-assembly checkpoint activity. Mediates cell death in response to chromosome missegregation and acts to suppress spontaneous tumorigenesis. -
組織特異性
High expression in testis and thymus, less in colon, spleen, lung and small intestine. Expressed in fetal thymus, bone marrow, heart, liver, spleen and thymus. Expression is associated with cells/tissues with a high mitotic index. -
配列類似性
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. BUB1 subfamily.
Contains 1 BUB1 N-terminal domain.
Contains 1 protein kinase domain. -
ドメイン
The KEN box is required for its ubiquitination and degradation.
BUB1 N-terminal domain directs kinetochore localization and binding to BUB3. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. Upon spindle-assembly checkpoint activation it is hyperphosphorylated and its kinase activity toward CDC20 is stimulated. Phosphorylation at Thr-609 is required for interaction with PLK1, phosphorylation at this site probably creates a binding site for the POLO-box domain of PLK1, thus enhancing the PLK1-BUB1 interaction.
Ubiquitinated and degraded during mitotic exit by APC/C-Cdh1. -
細胞内局在
Nucleus. Chromosome > centromere > kinetochore. Nuclear in interphase cells. Accumulates gradually during G1 and S phase of the cell cycle, peaks at G2/M, and drops dramatically after mitosis. Localizes to the outer kinetochore. Kinetochore localization is required for normal mitotic timing and checkpoint response to spindle damage and occurs very early in prophase. AURKB, CASC5 and INCENP are required for kinetochore localization. - Information by UniProt
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参照データベース
- Entrez Gene: 699 Human
- Omim: 602452 Human
- SwissProt: O43683 Human
- Unigene: 469649 Human
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別名
- Bub1 antibody
- BUB1 budding uninhibited by benzimidazoles 1 homolog antibody
- BUB1 budding uninhibited by benzimidazoles 1 homolog (yeast) antibody
see all
画像
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Western blot - Anti-Bub1 antibody (ab9000)Western blot using ab9000 on Bub1 antigen (lanes 1- 3), and HL-60 whole cell extract.
The Bub1 antigen is only a fragment of full length Bub1 and thus runs at a lower size than the band seen in HL-60 extract.Western blot using ab9000 on Bub1 antigen (lanes 1- 3), and HL-60 whole cell extract.
The Bub1 antigen is only a fragment of full length Bub1 and thus runs at a lower size than the band seen in HL-60 extract. -
Immunocytochemistry/ Immunofluorescence - Anti-Bub1 antibody (ab9000)Image from Sliedrecht T et al, PLoS One. 2010 Apr 22;5(4):e10251, Fig S4.ab9000 staining Bub1 in HCT 116 (Human colorectal carcinoma cell line) cells by Immunocytochemistry/ Immunofluorescence. Cells were plated on 12 mm coverslips, pre-extracted with 0.2% Triton X-100 in warm PEM (100 mM PIPES pH6.8, 1mM MgCl2 and 5mM EGTA) for 1 minute, fixed with 3% PFA in PBS, blocked with 3% BSA in PBS for 1 hour, incubated with primary antibody for 16 hrs at 4°C, washed with PBS/0.1% Triton X-100 and incubated with secondary antibody for an additional 1 hour at room temperature. ACA staining in red, Bub1 is green and DNA (DAPI) is in blue.
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Immunocytochemistry/ Immunofluorescence - Anti-Bub1 antibody (ab9000)Image from Cui W et al., PLoS One. 2012;7(2):e32044. Epub 2012 Feb 27. Fig 5.; doi:10.1371/journal.pone.0032044; February 27, 2012, PLoS ONE 7(2): e32044.ab9000, staining Bub1 in oocytes from Sprague-Dawley rats.
Oocytes were fixed in paraformaldehyde, permeabilized with 0.5% Triton-X 100 in PHEM and blocked in PHEM containing 3% BSA for 1 hour. Samples were incubated with primary antibody at 1/500 dilution overnight at 4°C. A Cy3-conjugated goat-anti-rabbit IgG (1/800) was used as the secondary antibody.
プロトコール
データシートおよび資料
参考文献 (18)
ab9000 は 18 報の論文で使用されています。
- Nyati S et al. TGFBR2 mediated phosphorylation of BUB1 at Ser-318 is required for transforming growth factor-ß signaling. Neoplasia 22:163-178 (2020). WB . PubMed: 32143140
- Alex A et al. Electroporated recombinant proteins as tools for in vivo functional complementation, imaging and chemical biology. Elife 8:N/A (2019). PubMed: 31310234
- Pagotto S et al. Hsa-miR-155-5p drives aneuploidy at early stages of cellular transformation. Oncotarget 9:13036-13047 (2018). IF ; Human . PubMed: 29560129
- Mur P et al. Germline mutations in the spindle assembly checkpoint genes BUB1 and BUB3 are infrequent in familial colorectal cancer and polyposis. Mol Cancer 17:23 (2018). IF ; Human . PubMed: 29448935
- Hengeveld RCC et al. Inner centromere localization of the CPC maintains centromere cohesion and allows mitotic checkpoint silencing. Nat Commun 8:15542 (2017). ICC/IF ; Human . PubMed: 28561035
