Anti-Brn-2 抗体 (ab101726)
Key features and details
- Goat polyclonal to Brn-2
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
製品の概要
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製品名
Anti-Brn-2 antibody
Brn-2 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to Brn-2 -
由来種
Goat -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse
交差が予測される動物種: Cow, Pig
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免疫原
Synthetic peptide corresponding to Human Brn-2 aa 37-52 (internal sequence).
Sequence:C-AQSLVQGDYGALQSN
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ポジティブ･コントロール
- ウェスタン・ブロット用ポジティブコントロールRecombinant Human Brn-2 protein
- WB: Human brain (cerebellum), mouse and rat brain tissue lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab101726 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab101726の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 47 kDa).
1 hour primary incubation is recommended for this product. |
| 特記事項 |
|---|
|
WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 47 kDa). 1 hour primary incubation is recommended for this product. |
ターゲット情報
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機能
Transcription factor that binds preferentially to the recognition sequence which consists of two distinct half-sites, ('GCAT') and ('TAAT'), separated by a nonconserved spacer region of 0, 2, or 3 nucleotides. Positively regulates the genes under the control of corticotropin-releasing hormone (CRH) and CRH II promoters. -
組織特異性
Expressed specifically in the neuroectodermal cell lineage. -
配列類似性
Belongs to the POU transcription factor family. Class-3 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 783320 Cow
- Entrez Gene: 18992 Mouse
- SwissProt: P31360 Mouse
- Unigene: 389520 Mouse
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別名
- Brain 2 antibody
- Brain specific homeobox/POU domain protein 2 antibody
- Brain-2 antibody
see all
画像
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Western blot - Anti-Brn-2 antibody (ab101726)Anti-Brn-2 antibody (ab101726) at 0.1 µg/ml + Mouse brain lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 47 kDa
Primary incubation was 1 hour.
データシートおよび資料
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Datasheet download
参考文献 (2)
ab101726 は 2 報の論文で使用されています。
- Lowenstein ED et al. Olig3 regulates early cerebellar development. Elife 10:N/A (2021). PubMed: 33591268
- Sakaeda M et al. Neural lineage-specific homeoprotein BRN2 is directly involved in TTF1 expression in small-cell lung cancer. Lab Invest : (2013). PubMed: 23358112
