Anti-BRI3 抗体 (ab101389)
Key features and details
- Rabbit polyclonal to BRI3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-BRI3 antibody -
製品の詳細
Rabbit polyclonal to BRI3 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Cow, Pig
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免疫原
Recombinant fragment corresponding to Human BRI3 aa 110-258.
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ポジティブ･コントロール
- HeLa, HepG2, MCF7 cell lines.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab101389の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 30 kDa.
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| IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Use 10mM Citrate buffer or Tris-EDTA buffer (PH8.0)
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| 特記事項 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 30 kDa. |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Use 10mM Citrate buffer or Tris-EDTA buffer (PH8.0) |
ターゲット情報
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機能
Negative regulator of beta amyloid peptide production. May inhibit the processing of APP by blocking its access to alpha-and beta-secretase. Binding to the beta-secretase-cleaved APP C-terminal fragment is negligible, suggesting that ITM2C is a poor gamma-secretase cleavage inhibitor. May play a role in TNF-induced cell death and neuronal differentiation. -
組織特異性
High levels in the brain, specifically in the cerebral cortex, medulla, amygdala, hippocampus, thalamus, caudate nucleus, cerebellum, olfactory lobe and spinal cord. Very low levels in other organs. -
配列類似性
Belongs to the ITM2 family.
Contains 1 BRICHOS domain. -
翻訳後修飾
Type I membrane-bound, as well as soluble, furin has a pre-eminent role in ITM2C proteolytic processing. PCSK7 and PCSK5 may also be involved although to a lesser extent. The soluble form of PCSK7 is incapable of processing ITM2C. -
細胞内局在
Lysosome membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 281256 Cow
- Entrez Gene: 539959 Cow
- Entrez Gene: 81618 Human
- Entrez Gene: 64294 Mouse
- Entrez Gene: 100144481 Pig
- Omim: 609554 Human
- SwissProt: A2VDN0 Cow
- SwissProt: Q9NQX7 Human
see all -
別名
- BRI3 antibody
- BRICD2C antibody
- BRICHOS domain containing 2C antibody
see all
画像
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Western blot - Anti-BRI3 antibody (ab101389)Anti-BRI3 antibody (ab101389) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 30 kDa
12% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRI3 antibody (ab101389)Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using ab101389 at 1/100 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab101389 は 2 報の論文で使用されています。
- Yasukawa T et al. NRBP1-Containing CRL2/CRL4A Regulates Amyloid ß Production by Targeting BRI2 and BRI3 for Degradation. Cell Rep 30:3478-3491.e6 (2020). PubMed: 32160551
- Dolfe L et al. The Bri2 and Bri3 BRICHOS Domains Interact Differently with Aß42 and Alzheimer Amyloid Plaques. J Alzheimers Dis Rep 2:27-39 (2018). PubMed: 30480246