Application
ChIP
Sample
Human Cell lysate - nuclear (HeLa cells)
Specification
HeLa cells
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Detection step
Real-time PCR
Positive control
I examined several cites that were reported to be occupied by BRG1, such as p53 and p21 promoters, CD44 transcriptional start site and transcribed region
Other product details
Dilution
1/60
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: IP buffer
Additional data
Additional Notes
The signal is very poor, only slightly above the background signal (determined by non-targeting control antibody). In most samples I got less than 0.01% of input.
Abcam response
The antibody is never tested in ChiP. We have no data to claim that the antibody will work in ChiP therefore we will welcome any feedback other customers have, regarding the ChiP application.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Efrat Shema
Verified customer
投稿 Apr 04 2012