製品の概要

  • 製品名

    Anti-BrdU antibody - Proliferation Marker
    BrdU 一次抗体 製品一覧
  • 製品の詳細

    Sheep polyclonal to BrdU - Proliferation Marker
  • 由来種

    Sheep
  • アプリケーション

    適用あり: IHC-FrFl, IHC-P, IHC-Fr, ICC/IF, ELISA, WB, IHC-FoFrmore details
  • 種交差性

    交差種: Species independent
  • 免疫原

    Other Immunogen Type corresponding to BrdU. Bromodeoxyuridine coupled to HGG (Human Gamma Globulin)

  • 特記事項

    Unstained positive control slides from mice treated with BrdU (formalin-fixed, paraffin-embedded intestine sections) are available as BrdU control slides ab129956

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab1893 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FrFl Use at an assay dependent concentration.
IHC-P Use a concentration of 10 µg/ml.
IHC-Fr Use a concentration of 10 µg/ml.
ICC/IF Use at an assay dependent concentration. PubMed: 21118958
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration. PubMed: 19332057

ターゲット情報

  • 関連性

    The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.
  • 細胞内局在

    Nuclear
  • 別名

    • Bromodeoxyuridine antibody
    • BUdr antibody

画像

  • Loss of PRC2 results in ectopic formation of Merkel cells around all hair follicle types.

    (Panel C) IF staining for BrdU shows no significant change in proliferation in the developing interfollicular epidermis (IFE) and hair follicles (HF) in E16 Ezh1/2 2KO and EED cKO compared to control.

  • Increased numbers of BrdU-labeled NPCs (neural progenitor cells ) with radial GFAP-positive processes in the DG GCL of CRAF ko at P10.

    (Panel A shown) Immunohistological analysis of BrdU (green) and the astrocytic marker GFAP (red) stained sagittal brain sections of CRAF ct and CRAF ko hippocampus at P10 24h after a single BrdU application. Representative brain sections of CRAF ct (upper panel) and CRAF ko (lower panel) show BrdU-labeled cells (green) colocalizing with GFAP-positive radial processes (red) (merge, white arrowheads). Scale bar = 50μm.

  • ab1893 staining BrdU in COS-7 (African green monkey kidney fibroblast-like cell line) cells by ICC/IF (Immunocytochemistry/immunofluorescence).

    Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 2% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in 2% BSA) for 1 hour at 25°C. ab96945, a DyLight® 594-conjugated rabbit anti-sheep IgG (H+L) polyclonal was used as the secondary antibody (1/200). Nuclei are stained blue with DAPI.

    See Abreview

  • ab1893 staining BrdU in Ramos (Human Burkitt's lymphoma cell line) cell line xenograft tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

    Tissue was fixed with formaldehyde and blocked with 15% serum for 1 hour at 20°C; antigen retrieval was by heat mediation in a sodium citrate buffer, pH 6. Samples were incubated with primary antibody (1/260 in TBS) for 18 hours at 20°C. An undiluted Alexa Fluor® 488-conjugated donkey anti-sheep IgG polyclonal was used as the secondary antibody.

    See Abreview

参考文献

This product has been referenced in:

  • Liang HX  et al. Neferine inhibits proliferation, migration and invasion of U251 glioma cells by down-regulation of miR-10b. Biomed Pharmacother 109:1032-1040 (2019). Read more (PubMed: 30551353) »
  • Gao W  et al. Ailanthone exerts anticancer effect by up-regulating miR-148a expression in MDA-MB-231 breast cancer cells and inhibiting proliferation, migration and invasion. Biomed Pharmacother 109:1062-1069 (2019). Read more (PubMed: 30551356) »
See all 117 Publications for this product

レビューと Q&A

1-10 of 22 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Brain SVZ)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
Yes - tritonX 0.05%
Specification
Brain SVZ
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

投稿 Oct 15 2018

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
None
Permeabilization
Yes - PBS + 0.2% Triton X-100 + 4% BSA
Specification
Brain
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 22°C
Fixative
Paraformaldehyde

Dr. Alexandre Magno

Verified customer

投稿 Aug 02 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (liver)
Antigen retrieval step
Heat mediated
Permeabilization
No
Specification
liver
Fixative
10% NBF

Abcam user community

Verified customer

投稿 Jun 27 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rhesus monkey Tissue sections (adrenal gland)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
No
Specification
adrenal gland
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: 23°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

投稿 Apr 21 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Ramos cell line xenograft)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: NaCitrate pH 6
Permeabilization
No
Specification
Ramos cell line xenograft
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: 20°C
Fixative
Formaldehyde

Abcam user community

Verified customer

投稿 Dec 25 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization
No
Specification
Liver
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

投稿 Aug 25 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Sample
African Green Monkey Cultured Cells (COS7)
Specification
COS7
Permeabilization
Yes - 0.5% TritonX100
Fixative
Paraformaldehyde

Abcam user community

Verified customer

投稿 Jan 27 2014

Answer

Unfortunately we do not have any cross-reactivity data against FDU.

Read More

Answer

Use 0.1M Borate buffer. Recipe is:

Sodium Tetraborate (fw =381.4) --> 38.14 grams to 1000 ml water, pH 8.5

After the soaking in the Borate buffer (pH 8.5) they are neutralized by rinsing in the PBS buffer (pH 7.4), which is listed on both protocols

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Question
Answer

Cultured cells should be pulsed with 10uM BrdU for 1 hour - overnight.

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1-10 of 22 Abreviews or Q&A

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