Anti-BrdU 抗体 - Proliferation Marker (ab1893)
製品の概要
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製品名
Anti-BrdU antibody - Proliferation Marker
BrdU 一次抗体 製品一覧 -
製品の詳細
Sheep polyclonal to BrdU - Proliferation Marker -
由来種
Sheep -
アプリケーション
適用あり: IHC-FrFl, IHC-P, IHC-Fr, ICC/IF, ELISA, WB, IHC-FoFrmore details -
種交差性
交差種: Species independent -
免疫原
Other Immunogen Type corresponding to BrdU. Bromodeoxyuridine coupled to HGG (Human Gamma Globulin)
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特記事項
Unstained positive control slides from mice treated with BrdU (formalin-fixed, paraffin-embedded intestine sections) are available as BrdU control slides ab129956.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.6
Constituent: 0.4% PBS -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Isotype control
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Related Products
アプリケーション
Our Abpromise guarantee covers the use of ab1893 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-FrFl | Use at an assay dependent concentration. | |
| IHC-P | Use a concentration of 10 µg/ml. | |
| IHC-Fr | Use a concentration of 10 µg/ml. | |
| ICC/IF | Use at an assay dependent concentration. PubMed: 21118958 | |
| ELISA | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. | |
| IHC-FoFr | Use at an assay dependent concentration. PubMed: 19332057 |
ターゲット情報
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関連性
The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis. -
細胞内局在
Nuclear -
別名
- Bromodeoxyuridine antibody
- BUdr antibody
画像
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Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody - Proliferation Marker (ab1893)Perdigoto et al PLoS Genet. 2016 Jul 14;12(7):e1006151. doi: 10.1371/journal.pgen.1006151. eCollection 2016 Jul. Fig S5. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Loss of PRC2 results in ectopic formation of Merkel cells around all hair follicle types.
(Panel C) IF staining for BrdU shows no significant change in proliferation in the developing interfollicular epidermis (IFE) and hair follicles (HF) in E16 Ezh1/2 2KO and EED cKO compared to control.
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Immunohistochemistry (Frozen sections) - Anti-BrdU antibody - Proliferation Marker (ab1893)Pfeiffer et al PLoS One. 2018 Mar 28;13(3):e0192067. doi: 10.1371/journal.pone.0192067. eCollection 2018. Fig 4. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Increased numbers of BrdU-labeled NPCs (neural progenitor cells ) with radial GFAP-positive processes in the DG GCL of CRAF ko at P10.
(Panel A shown) Immunohistological analysis of BrdU (green) and the astrocytic marker GFAP (red) stained sagittal brain sections of CRAF ct and CRAF ko hippocampus at P10 24h after a single BrdU application. Representative brain sections of CRAF ct (upper panel) and CRAF ko (lower panel) show BrdU-labeled cells (green) colocalizing with GFAP-positive radial processes (red) (merge, white arrowheads). Scale bar = 50μm.
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Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody - Proliferation Marker (ab1893)This image is courtesy of an anonymous Abreviewab1893 staining BrdU in COS-7 (African green monkey kidney fibroblast-like cell line) cells by ICC/IF (Immunocytochemistry/immunofluorescence).
Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 2% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in 2% BSA) for 1 hour at 25°C. ab96945, a DyLight® 594-conjugated rabbit anti-sheep IgG (H+L) polyclonal was used as the secondary antibody (1/200). Nuclei are stained blue with DAPI.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody - Proliferation Marker (ab1893)This image is courtesy of an anonymous Abreviewab1893 staining BrdU in Ramos (Human Burkitt's lymphoma cell line) cell line xenograft tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Tissue was fixed with formaldehyde and blocked with 15% serum for 1 hour at 20°C; antigen retrieval was by heat mediation in a sodium citrate buffer, pH 6. Samples were incubated with primary antibody (1/260 in TBS) for 18 hours at 20°C. An undiluted Alexa Fluor® 488-conjugated donkey anti-sheep IgG polyclonal was used as the secondary antibody.
参考文献
This product has been referenced in:
- Liang HX et al. Neferine inhibits proliferation, migration and invasion of U251 glioma cells by down-regulation of miR-10b. Biomed Pharmacother 109:1032-1040 (2019). Read more (PubMed: 30551353) »
- Gao W et al. Ailanthone exerts anticancer effect by up-regulating miR-148a expression in MDA-MB-231 breast cancer cells and inhibiting proliferation, migration and invasion. Biomed Pharmacother 109:1062-1069 (2019). Read more (PubMed: 30551356) »
