Anti-BrdU 抗体 - Proliferation Marker (ab1893)
Key features and details
- Sheep polyclonal to BrdU - Proliferation Marker
- Suitable for: IHC-FrFl, IHC-P, IHC-Fr, ELISA, WB
- Reacts with: Species independent
- Isotype: IgG
製品の概要
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製品名
Anti-BrdU antibody - Proliferation Marker
BrdU 一次抗体 製品一覧 -
製品の詳細
Sheep polyclonal to BrdU - Proliferation Marker -
由来種
Sheep -
アプリケーション
適用あり: IHC-FrFl, IHC-P, IHC-Fr, ELISA, WBmore details -
種交差性
交差種: Species independent -
免疫原
Chemical/ Small Molecule corresponding to BrdU. Coupled to HGG (Human Gamma Globulin).
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特記事項
Unstained positive control slides from mice treated with BrdU (formalin-fixed, paraffin-embedded intestine sections) are available as BrdU control slides ab129956.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.60
Constituent: 0.4% PBS -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab1893の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-FrFl | (1) |
Use at an assay dependent concentration.
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| IHC-P | (8) |
Use a concentration of 10 µg/ml.
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| IHC-Fr | (2) |
Use a concentration of 10 µg/ml.
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| ELISA |
Use at an assay dependent concentration.
|
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| WB |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
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IHC-FrFl
Use at an assay dependent concentration. |
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IHC-P
Use a concentration of 10 µg/ml. |
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IHC-Fr
Use a concentration of 10 µg/ml. |
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ELISA
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. |
ターゲット情報
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関連性
The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis. -
細胞内局在
Nuclear -
別名
- Bromodeoxyuridine antibody
- BUdr antibody
画像
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Immunohistochemistry (Frozen sections) - Anti-BrdU antibody - Proliferation Marker (ab1893)Pfeiffer et al PLoS One. 2018 Mar 28;13(3):e0192067. doi: 10.1371/journal.pone.0192067. eCollection 2018. Fig 4. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Increased numbers of BrdU-labeled NPCs (neural progenitor cells ) with radial GFAP-positive processes in the DG GCL of CRAF ko at P10.
(Panel A shown) Immunohistological analysis of BrdU (green) and the astrocytic marker GFAP (red) stained sagittal brain sections of CRAF ct and CRAF ko hippocampus at P10 24h after a single BrdU application. Representative brain sections of CRAF ct (upper panel) and CRAF ko (lower panel) show BrdU-labeled cells (green) colocalizing with GFAP-positive radial processes (red) (merge, white arrowheads). Scale bar = 50μm.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody - Proliferation Marker (ab1893)This image is courtesy of an anonymous Abreviewab1893 staining BrdU in Ramos (Human Burkitt's lymphoma cell line) cell line xenograft tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Tissue was fixed with formaldehyde and blocked with 15% serum for 1 hour at 20°C; antigen retrieval was by heat mediation in a sodium citrate buffer, pH 6. Samples were incubated with primary antibody (1/260 in TBS) for 18 hours at 20°C. An undiluted Alexa Fluor® 488-conjugated donkey anti-sheep IgG polyclonal was used as the secondary antibody.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (151)
ab1893 は 151 報の論文で使用されています。
- Shimizu Y & Kawasaki T Histone acetyltransferase EP300 regulates the proliferation and differentiation of neural stem cells during adult neurogenesis and regenerative neurogenesis in the zebrafish optic tectum. Neurosci Lett 756:135978 (2021). PubMed: 34023416
- Recabal A et al. The FGF2-induced tanycyte proliferation involves a connexin 43 hemichannel/purinergic-dependent pathway. J Neurochem 156:182-199 (2021). PubMed: 32936929
- Jang H et al. FCN3 functions as a tumor suppressor of lung adenocarcinoma through induction of endoplasmic reticulum stress. Cell Death Dis 12:407 (2021). PubMed: 33859174
- Li B et al. Apelin/APJ relieve diabetic cardiomyopathy by reducing microvascular dysfunction. J Endocrinol 249:1-18 (2021). PubMed: 33504680
- Vinsland E et al. The zinc finger/RING domain protein Unkempt regulates cognitive flexibility. Sci Rep 11:16299 (2021). PubMed: 34381067