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  1. Link

    brdu-antibody-proliferation-marker-ab1893.pdf

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Anti-BrdU 抗体 - Proliferation Marker (ab1893)

  • Datasheet
  • SDS
Reviews (15)Q&A (7)References (135)

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出荷および受注について

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Immunohistochemistry (Frozen sections) - Anti-BrdU antibody - Proliferation Marker (ab1893)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody - Proliferation Marker (ab1893)

Key features and details

  • Sheep polyclonal to BrdU - Proliferation Marker
  • Suitable for: IHC-FrFl, IHC-P, IHC-Fr, ELISA, WB
  • Reacts with: Species independent
  • Isotype: IgG

こちらの製品もご検討ください

Biochemical
Product image
BrdU (5-bromo-2'-deoxyuridine), Thymidine analog (ab142567)

関連製品

製品の概要

  • 製品名

    Anti-BrdU antibody - Proliferation Marker
    BrdU 一次抗体 製品一覧
  • 製品の詳細

    Sheep polyclonal to BrdU - Proliferation Marker
  • 由来種

    Sheep
  • アプリケーション

    適用あり: IHC-FrFl, IHC-P, IHC-Fr, ELISA, WBmore details
  • 種交差性

    交差種: Species independent
  • 免疫原

    Other Immunogen Type corresponding to BrdU. Bromodeoxyuridine coupled to HGG (Human Gamma Globulin)

  • 特記事項

    Unstained positive control slides from mice treated with BrdU (formalin-fixed, paraffin-embedded intestine sections) are available as BrdU control slides ab129956. 

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー

    pH: 7.60
    Constituent: 0.4% PBS
  • Concentration information loading...
  • 精製度

    Protein G purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Cell Biology
    • Cell Cycle
    • Markers
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
    • Tags & Cell Markers
    • Cell Type Markers
    • Replication
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA / Nucleotides

関連製品

  • Isotype control

    • Sheep IgG - Isotype Control (ab37385)
  • Related Products

    • BrdU (5-bromo-2'-deoxyuridine), Thymidine analog (ab142567)
    • Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (ab6326)

アプリケーション

Our Abpromise guarantee covers the use of ab1893 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FrFl Use at an assay dependent concentration.
IHC-P Use a concentration of 10 µg/ml.
IHC-Fr Use a concentration of 10 µg/ml.
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

ターゲット情報

  • 関連性

    The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.
  • 細胞内局在

    Nuclear
  • 別名

    • Bromodeoxyuridine antibody
    • BUdr antibody

画像

  • Immunohistochemistry (Frozen sections) - Anti-BrdU antibody - Proliferation Marker (ab1893)
    Immunohistochemistry (Frozen sections) - Anti-BrdU antibody - Proliferation Marker (ab1893)Pfeiffer et al PLoS One. 2018 Mar 28;13(3):e0192067. doi: 10.1371/journal.pone.0192067. eCollection 2018. Fig 4. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Increased numbers of BrdU-labeled NPCs (neural progenitor cells ) with radial GFAP-positive processes in the DG GCL of CRAF ko at P10.

    (Panel A shown) Immunohistological analysis of BrdU (green) and the astrocytic marker GFAP (red) stained sagittal brain sections of CRAF ct and CRAF ko hippocampus at P10 24h after a single BrdU application. Representative brain sections of CRAF ct (upper panel) and CRAF ko (lower panel) show BrdU-labeled cells (green) colocalizing with GFAP-positive radial processes (red) (merge, white arrowheads). Scale bar = 50μm.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody - Proliferation Marker (ab1893)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody - Proliferation Marker (ab1893)This image is courtesy of an anonymous Abreview

    ab1893 staining BrdU in Ramos (Human Burkitt's lymphoma cell line) cell line xenograft tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

    Tissue was fixed with formaldehyde and blocked with 15% serum for 1 hour at 20°C; antigen retrieval was by heat mediation in a sodium citrate buffer, pH 6. Samples were incubated with primary antibody (1/260 in TBS) for 18 hours at 20°C. An undiluted Alexa Fluor® 488-conjugated donkey anti-sheep IgG polyclonal was used as the secondary antibody.

    See Abreview

プロトコール

  • Recommended protocol for BrdU staining with ab1893

Click here to view the general protocols

データシートおよび資料

    • Datasheet
    • SDS
  • 参考文献 (135)

    ab1893 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab1893 は 135 報の論文で使用されています。

    • Cao X  et al. Soluble receptor for advanced glycation end-products promotes angiogenesis through activation of STAT3 in myocardial ischemia/reperfusion injury. Apoptosis 25:341-353 (2020). PubMed: 32333220
    • Kim CG  et al. Effects of Balneotherapy in Jeju Magma-Seawater on Knee Osteoarthritis Model. Sci Rep 10:6620 (2020). PubMed: 32313003
    • Liskiewicz A  et al. Physical activity reduces anxiety and regulates brain fatty acid synthesis. Mol Brain 13:62 (2020). PubMed: 32303271
    • Fu DL  et al. Sanhua Decoction, a Classic Herbal Prescription, Exerts Neuroprotection Through Regulating Phosphorylated Tau Level and Promoting Adult Endogenous Neurogenesis After Cerebral Ischemia/Reperfusion Injury. Front Physiol 11:57 (2020). PubMed: 32116767
    • Ostasov P  et al. Sonic Hedgehog and Triiodothyronine Pathway Interact in Mouse Embryonic Neural Stem Cells. Int J Mol Sci 21:N/A (2020). PubMed: 32456161
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 22 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-BrdU antibody - Proliferation Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Brain SVZ)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer
    Permeabilization
    Yes - tritonX 0.05%
    Specification
    Brain SVZ
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Oct 15 2018

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-BrdU antibody - Proliferation Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Antigen retrieval step
    None
    Permeabilization
    Yes - PBS + 0.2% Triton X-100 + 4% BSA
    Specification
    Brain
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Dr. Alexandre Magno

    Verified customer

    投稿 Aug 02 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-BrdU antibody - Proliferation Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (liver)
    Antigen retrieval step
    Heat mediated
    Permeabilization
    No
    Specification
    liver
    Fixative
    10% NBF
    Read More

    Abcam user community

    Verified customer

    投稿 Jun 27 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-BrdU antibody - Proliferation Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rhesus monkey Tissue sections (adrenal gland)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate
    Permeabilization
    No
    Specification
    adrenal gland
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Apr 21 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-BrdU antibody - Proliferation Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Ramos cell line xenograft)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: NaCitrate pH 6
    Permeabilization
    No
    Specification
    Ramos cell line xenograft
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Dec 25 2015

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-BrdU antibody - Proliferation Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer
    Permeabilization
    No
    Specification
    Liver
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Aug 25 2015

    Immunocytochemistry/ Immunofluorescence abreview for Anti-BrdU antibody - Proliferation Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Sample
    African Green Monkey Cultured Cells (COS7)
    Specification
    COS7
    Permeabilization
    Yes - 0.5% TritonX100
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Jan 27 2014

    Question

    I see this kit has not been tested yet against IDU, do you have any testing data for FDU cross-reactivity?

    Read More

    Abcam community

    Verified customer

    Asked on Mar 11 2013

    Answer

    Unfortunately we do not have any cross-reactivity data against FDU.

    Read More

    Abcam Scientific Support

    Answered on Mar 11 2013

    Question

    The protocol with this kit states to use borate buffer, point 5 of the protocol.
    We do not have this buffer in our lab. Is there any other buffer we can use? Or do you have the recipe for this one?

    'Immediately after the acid washes Borate buffer (0.1M) is then used to buffer the cells for 12 minutes at r.t'
    How do we neutralize this afterwards?

    Read More

    Abcam community

    Verified customer

    Asked on Nov 30 2012

    Answer

    Use 0.1M Borate buffer. Recipe is:

    Sodium Tetraborate (fw =381.4) --> 38.14 grams to 1000 ml water, pH 8.5

    After the soaking in the Borate buffer (pH 8.5) they are neutralized by rinsing in the PBS buffer (pH 7.4), which is listed on both protocols

    Read More

    Abcam Scientific Support

    Answered on Nov 30 2012

    Question

    How long should I treat my cultured cells with BrdU and at what concentration?

    Read More

    Abcam community

    Verified customer

    Asked on Sep 07 2012

    Answer

    Cultured cells should be pulsed with 10uM BrdU for 1 hour - overnight.

    Read More

    Abcam Scientific Support

    Answered on Sep 07 2012

    1-10 of 22 Abreviews or Q&A

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