Anti-BrdU 抗体 [IIB5] (ab8955)
![Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody [IIB5] (ab8955)](https://www.abcam.co.jp/ps/products/8/ab8955/Images/ab8955-287553-anti-brdu-antibody-iib5-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody [IIB5] (ab8955)")
Key features and details
- Mouse monoclonal [IIB5] to BrdU
- Suitable for: ICC/IF, Flow Cyt, IHC-Fr, IHC-P
- Reacts with: Species independent
- Isotype: IgG1
製品の概要
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製品名
Anti-BrdU antibody [IIB5]
BrdU 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [IIB5] to BrdU -
由来種
Mouse -
特異性
This antibody also reacts with bromodeoxyuridine when incorporated into nuclear DNA. It cross reacts with iododeoxyuridine (IdU). -
アプリケーション
適用あり: ICC/IF, Flow Cyt, IHC-Fr, IHC-Pmore details -
種交差性
交差種: Species independent -
免疫原
Chemical/ Small Molecule corresponding to BrdU conjugated to bovine serum albumin.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
IIB5 -
ミエローマ
Sp2/0-Ag14 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab8955の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
Using immunocytochemistry, a combination of this antibody and Mouse monoclonal to Mitotic Cells (ab8956) can distinguish and quantitate the four major fractions of the cell cycle. |
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| Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| IHC-Fr |
1/100 - 1/200. ABC method.
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| IHC-P | (1) |
1/100 - 1/200. ABC method.
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| 特記事項 |
|---|
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ICC/IF
Use at an assay dependent concentration. Using immunocytochemistry, a combination of this antibody and Mouse monoclonal to Mitotic Cells (ab8956) can distinguish and quantitate the four major fractions of the cell cycle. |
|
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
IHC-Fr
1/100 - 1/200. ABC method. |
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IHC-P
1/100 - 1/200. ABC method. |
ターゲット情報
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関連性
The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis. -
細胞内局在
Nuclear -
別名
- Bromodeoxyuridine antibody
- BUdr antibody
画像
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Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody [IIB5] (ab8955)
Indirect immunofluorescence staining of BrdU-labeled MR65 lung cancer cells with ab8955.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [IIB5] (ab8955)](https://www.abcam.co.jp/ps/products/8/ab8955/Images/ab8955-301028-anti-brdu-antibody-iib5-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [IIB5] (ab8955)BrdU staining in sponge Halisarca caerulea using ab8955. Metacrylate embedded tissue sections were incubated with 1:200 diluted primary antibody. An avidin-biotin complex kit using biotinylated rabbit anti-mouse antibody and then incubation in avidin-biotin-peroxidase complex was used for detection.
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![Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody [IIB5] (ab8955)](https://www.abcam.co.jp/ps/products/8/ab8955/Images/ab8955-391620-anti-brdu-antibody-iib5-immunocytochemistry-mr65.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody [IIB5] (ab8955)Indirect immunofluorescence staining of BrdU-labeled MR65 lung cancer cells using ab8955 (1:1000).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [IIB5] (ab8955)](https://www.abcam.co.jp/ps/products/8/ab8955/Images/ab8955-301027-anti-brdu-antibody-iib5-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [IIB5] (ab8955)BrdU staining in paraffin embedded rat intestine using ab8955. Primary antibody diluted 1:200. Peroxidase-conjugated goat anti mouse secondary antibody was used for detection.
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![Flow Cytometry - Anti-BrdU antibody [IIB5] (ab8955)](https://www.abcam.co.jp/ps/products/8/ab8955/Images/ab8955-301026-anti-brdu-antibody-iib5-flow-cytometry.jpg)
Flow Cytometry - Anti-BrdU antibody [IIB5] (ab8955)Flow cytometric BrdU-DNA cell cycle analysis in a lung cancer cell line. Dilution 1:100 of primary antibody ab8955. FITC-conjugated goat anti mouse secondary antibody was used for detection.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (23)
ab8955 は 23 報の論文で使用されています。
- Naveed A et al. NEAT1 polyA-modulating antisense oligonucleotides reveal opposing functions for both long non-coding RNA isoforms in neuroblastoma. Cell Mol Life Sci 78:2213-2230 (2021). PubMed: 32914209
- Scheckel C et al. Ribosomal profiling during prion disease uncovers progressive translational derangement in glia but not in neurons. Elife 9:N/A (2020). PubMed: 32960170
- Tournaire G et al. Nestin-GFP transgene labels skeletal progenitors in the periosteum. Bone 133:115259 (2020). PubMed: 32036051
- Meng CY et al. MicroRNA-22 mediates the cisplatin resistance of osteosarcoma cells by inhibiting autophagy via the PI3K/Akt/mTOR pathway. Oncol Rep 43:1169-1186 (2020). PubMed: 32323781
- Kwart D et al. A Large Panel of Isogenic APP and PSEN1 Mutant Human iPSC Neurons Reveals Shared Endosomal Abnormalities Mediated by APP ß-CTFs, Not Aß. Neuron 104:256-270.e5 (2019). PubMed: 31416668
