Anti-BrdU 抗体 [IIB5] (ab8955)

Reviews (3)Q&A (1)References (16)

Key features and details

  • Mouse monoclonal [IIB5] to BrdU
  • Suitable for: ICC/IF, Flow Cyt, IHC-Fr, IHC-P
  • Reacts with: Species independent
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-BrdU antibody [IIB5]
    BrdU 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [IIB5] to BrdU

  • 由来種

    Mouse

  • 特異性

    This antibody also reacts with bromodeoxyuridine when incorporated into nuclear DNA. It cross reacts with iododeoxyuridine (IdU).

  • アプリケーション

    適用あり: ICC/IF, Flow Cyt, IHC-Fr, IHC-Pmore details

  • 種交差性

    交差種: Species independent

  • 免疫原

    Chemical/ Small Molecule conjugated to BSA.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab8955 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.

Using immunocytochemistry, a combination of this antibody and Mouse monoclonal to Mitotic Cells (ab8956) can distinguish and quantitate the four major fractions of the cell cycle.
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr 1/100 - 1/200. ABC method.
IHC-P 1/100 - 1/200. ABC method.

ターゲット情報

  • 関連性

    The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.

  • 細胞内局在

    Nuclear

  • 別名

    • Bromodeoxyuridine antibody
    • BUdr antibody

画像

  • Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody [IIB5] (ab8955)
    Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody [IIB5] (ab8955)

    Indirect immunofluorescence staining of BrdU-labeled MR65 lung cancer cells with ab8955.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [IIB5] (ab8955)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [IIB5] (ab8955)

    BrdU staining in sponge Halisarca caerulea using ab8955. Metacrylate embedded tissue sections were incubated with 1:200 diluted primary antibody. An avidin-biotin complex kit using biotinylated rabbit anti-mouse antibody and then incubation in avidin-biotin-peroxidase complex was used for detection. 

  • Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody [IIB5] (ab8955)
    Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody [IIB5] (ab8955)

    Indirect immunofluorescence staining of BrdU-labeled MR65 lung cancer cells using ab8955 (1:1000).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [IIB5] (ab8955)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [IIB5] (ab8955)

    BrdU staining in paraffin embedded rat intestine using ab8955. Primary antibody diluted 1:200.  Peroxidase-conjugated goat anti mouse secondary antibody was used for detection.

  • Flow Cytometry - Anti-BrdU antibody [IIB5] (ab8955)
    Flow Cytometry - Anti-BrdU antibody [IIB5] (ab8955)

    Flow cytometric BrdU-DNA cell cycle analysis in a lung cancer cell line. Dilution 1:100 of primary antibody ab8955. FITC-conjugated goat anti mouse secondary antibody was used for detection.

参考文献 (16)

ab8955 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab8955 は 16 報の論文で使用されています。

  • Meng CY  et al. MicroRNA-22 mediates the cisplatin resistance of osteosarcoma cells by inhibiting autophagy via the PI3K/Akt/mTOR pathway. Oncol Rep 43:1169-1186 (2020). PubMed: 32323781
  • Collinson JM  et al. The developmental and genetic basis of 'clubfoot' in the peroneal muscular atrophy mutant mouse. Development 145:N/A (2018). PubMed: 29439133
  • Sagga N  et al. Limbal epithelial stem cell activity and corneal epithelial cell cycle parameters in adult and aging mice. Stem Cell Res 33:185-198 (2018). PubMed: 30439642
  • Niu H  et al. Effect of Long-Term Sodium Salicylate Administration on Learning, Memory, and Neurogenesis in the Rat Hippocampus. Biomed Res Int 2018:7807426 (2018). PubMed: 29805976
  • Duan X  et al. Early changes in the knee of healer and non-healer mice following non-invasive mechanical injury. J Orthop Res 35:524-536 (2017). PubMed: 27591401
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1-4 of 4 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-BrdU antibody [IIB5]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain, cortex section 60um)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 2M HCl 37 deg
Permeabilization
Yes - 0.25% tritonX-100
Specification
Brain, cortex section 60um
Blocking step
2%BSA + 10% Horse serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative
Paraformaldehyde
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Ms. Lynette Lim

Verified customer

投稿 Mar 20 2020

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-BrdU antibody [IIB5]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (brain)
Antigen retrieval step
Other
Permeabilization
Yes - 0.3% triton x-100
Specification
brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3 · Temperature: 23°C
Fixative
Paraformaldehyde
Read More

Jacob Garza

Verified customer

投稿 Sep 14 2017

Question

Do you have any data to indicate that ab8955 crossreacts with chlorodeoxyuridine?

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Answer

We unfortunately do not have any data confirming its cross reactivity with chlorodeoxyuridine. We however have customer feedback that say it does not bind to CldU or Idu but there is no tested data available.

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Immunocytochemistry abreview for Anti-BrdU antibody [IIB5] - Proliferation Marker

 Average
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Rat Cultured Cells (BMSC)
Specification
BMSC
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton X100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Read More

Abcam user community

Verified customer

投稿 Apr 22 2008

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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