製品の概要

  • 製品名
    Anti-BrdU antibody (HRP)
    BrdU 一次抗体 製品一覧
  • 製品の詳細
    Sheep polyclonal to BrdU (HRP)
  • 由来種
    Sheep
  • 標識
    HRP
  • 特異性
    The antibody was tested using immunoprecipitation against 5-Methyl Cytosine (5-MeC) and bromo-deoxyuridine (BrdU) or control (no antigen) and assayed by A-405 spectrophotometry. At a concentration of 25 µg/mL, this product demonstrates 8 fold higher reactivity against BrdU than 5-MeC. This product titers out at 50 ng/mL. For best results, use the product at a concentration of 25 to 100 µg/mL. Nearly complete immunoprecipitation was obtained at a concentration of 100 to 500 µg/mL. This antibody is not species specific. The nucleotide sequence is present in all species.
  • アプリケーション
    適用あり: ELISA, IHC-P, IHC-Fr, IP, WBmore details
  • 種交差性
    交差種: a wide range of other species
  • 免疫原

    Bromodeoxyuridine coupled to keyhole limpet hemocyanin (KLH)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab2285 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

ターゲット情報

  • 関連性
    The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.
  • 細胞内局在
    Nuclear
  • 別名
    • Bromodeoxyuridine antibody
    • BUdr antibody

参考文献

This product has been referenced in:
  • Sano H  et al. Identification of 5-methylcytosine in DNA fragments immobilized on nitrocellulose paper. Proc Natl Acad Sci U S A 77:3581-5 (1980). Read more (PubMed: 6251470) »

See 1 Publication for this product

レビューと Q&A

Thank you for your inquiry. Yes, I can confirm that you are absolutely right, you will just need the DAB.

Indeed, HRP is the enzyme (horse radish peroxidase) which will convert the substrate (DAB) into the colored precipitation seen on the sl...

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The laboratory has used this antibody at a concentration of 10 µg/mL to obtain a good signal, comparable to other antibodies. As the antibody is at a concentration of 0.5mg/ml, this corresponds to a dilution of 1/50.

We do however recommend t...

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The actual protocols used can be found in the following publication. Campana D, Coustan-Smith E., Janossy G., Department of Immunology, Royal Free Hospital School of Medicine, London, UK. Double and triple staining methods for studying the prolifer...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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