Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Nothobranchius furzeri Tissue sections (brain)
Antigen retrieval step
Other
Permeabilization
Yes - TBS
Specification
brain
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 22°C
Fixative
Paraformaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: TNB
Dilution
1/1000
Secondary antibody
Dilution
1/300
Name
Abcam antibody:Goat Anti-Rat IgG H&L (Alexa Fluor® 594) preadsorbed
Additional data
Additional Notes
Photo shows BrdU positive cells in the ventricular zone of the telencephalon of N. furzeri at 1 day post BrdU injection.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Jolien Van houcke
Verified customer
投稿 Jul 05 2019