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Anti-Brd4 抗体 [BL-149-2H5] - BSA free (ab272042)

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ChIP-sequencing - Anti-Brd4 antibody [BL-149-2H5] - BSA free (ab272042)
  • Immunocytochemistry/ Immunofluorescence - Anti-Brd4 antibody [BL-149-2H5] - BSA free (ab272042)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brd4 antibody [BL-149-2H5] - BSA free (ab272042)
  • Immunoprecipitation - Anti-Brd4 antibody [BL-149-2H5] - BSA free (ab272042)
  • Anti-Brd4 antibody [BL-149-2H5] - BSA free (ab272042)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [BL-149-2H5] to Brd4 - BSA free
  • Suitable for: WB, IP, IHC-P, ChIP-sequencing, ICC/IF
  • Reacts with: Human

製品の概要

  • 製品名

    Anti-Brd4 antibody [BL-149-2H5] - BSA free
    Brd4 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [BL-149-2H5] to Brd4 - BSA free

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    ChIP-seq
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    See all applications and species data

  • 免疫原

    Synthetic peptide within Human Brd4 aa 1312-1362. The exact sequence is proprietary. NP_490597.1 and Gene ID 23476.
    Database link: O60885

  • 特記事項

    This product is sold under License from Bethyl Laboratories, Inc.

    ab272042 is the carrier-free version of ab243862. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

製品の特性

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab272042 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
ChIP-seq
Human
ICC/IF
Human
IHC-P
Human
IP
Human
アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ChIP-sequencing
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ChIP-sequencing
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Plays a role in a process governing chromosomal dynamics during mitosis.

  • 組織特異性

    Ubiquitously expressed.

  • 関連疾患

    Note=A chromosomal aberration involving BRD4 is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. Translocation t(15;19)(q14;p13) with NUT which produces a BRD4-NUT fusion protein.

  • 配列類似性

    Contains 2 bromo domains.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Brd4 antibody
      • BRD4-NUT FUSION antibody
      • BRD4-NUT fusion oncoprotein antibody
      • BRD4_HUMAN antibody
      • Bromodomain containing 4 antibody
      • bromodomain containing protein 4 antibody
      • Bromodomain-containing protein 4 antibody
      • CAP antibody
      • chromosome associated protein antibody
      • HUNK1 antibody
      • HUNKI antibody
      • MCAP antibody
      • Mitotic chromosome-associated protein antibody
      • Protein HUNK1 antibody
      see all

    画像

    • ChIP-sequencing - Anti-Brd4 antibody [BL-149-2H5] - BSA free (ab272042)
      ChIP-sequencing - Anti-Brd4 antibody [BL-149-2H5] - BSA free (ab272042)
      Localization of human BRD4 binding sites in immunoprecipitates from Mia PaCa-2 cells by ChIP-Seq using ab243862.

      This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243862).

    • Immunocytochemistry/ Immunofluorescence - Anti-Brd4 antibody [BL-149-2H5] - BSA free (ab272042)
      Immunocytochemistry/ Immunofluorescence - Anti-Brd4 antibody [BL-149-2H5] - BSA free (ab272042)
      Formaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling brd4 using ab243862 at 1/250 dilution in ICC/IF analysis. DyLight® 594-conjugated goat anti-rabbit IgG was used as the secondary antibody.

      This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243862).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brd4 antibody [BL-149-2H5] - BSA free (ab272042)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brd4 antibody [BL-149-2H5] - BSA free (ab272042)
      Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for brd4 using ab243862 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.

      This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243862).

    • Immunoprecipitation - Anti-Brd4 antibody [BL-149-2H5] - BSA free (ab272042)
      Immunoprecipitation - Anti-Brd4 antibody [BL-149-2H5] - BSA free (ab272042)

      brd4 was immunoprecipitated from 1 mg HeLa whole cell lysate with ab243862 at 20 µL/mg lysate. Western blot was performed on the immunoprecipitate using ab243862 at 1/1000 dilution.

      Lane 1: ab243862 IP in HeLa cell lysate.

      Lane 2: Contol IgG in HeLa cell lysate.

      This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243862).

    • Anti-Brd4 antibody [BL-149-2H5] - BSA free (ab272042)
      Anti-Brd4 antibody [BL-149-2H5] - BSA free (ab272042)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab272042 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab272042 は論文での使用が確認できていません。

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