Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse neural stem cells)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
25 µg
Specification
mouse neural stem cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: IRDYE800
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Detection
Detection method
Fluorescence
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 105 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Robert Kupp
Verified customer
投稿 Oct 19 2020