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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-BRCC36 抗体 [EPR4365] (ab108295)

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Western blot - Anti-BRCC36 antibody [EPR4365] (ab108295)
  • Western blot - Anti-BRCC36 antibody [EPR4365] (ab108295)
  • Anti-BRCC36 antibody [EPR4365] (ab108295)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4365] to BRCC36
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-BRCC36 antibody [EPR4365]
    BRCC36 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR4365] to BRCC36

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WBmore details
    適用なし: Flow Cyt or IHC-P

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • HAP1, MCF7, SKBR3, 293T, Human fetal kidney and HeLa whole cell lysate (ab150035).

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab108295の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 36 kDa.
特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 36 kDa.
追加情報
Is unsuitable for Flow Cyt or IHC-P.

ターゲット情報

  • 機能

    Metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains (PubMed:19214193, PubMed:20656690, PubMed:24075985, PubMed:26344097). Does not have activity toward 'Lys-48'-linked polyubiquitin chains. Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). In the BRCA1-A complex, it specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX, antagonizing the RNF8-dependent ubiquitination at double-strand breaks (DSBs) (PubMed:20656690). Catalytic subunit of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin in various substrates (PubMed:20656690, PubMed:24075985, PubMed:26344097, PubMed:26195665). Mediates the specific 'Lys-63'-specific deubiquitination associated with the COP9 signalosome complex (CSN), via the interaction of the BRISC complex with the CSN complex (PubMed:19214193). The BRISC complex is required for normal mitotic spindle assembly and microtubule attachment to kinetochores via its role in deubiquitinating NUMA1 (PubMed:26195665). Plays a role in interferon signaling via its role in the deubiquitination of the interferon receptor IFNAR1; deubiquitination increases IFNAR1 activity by enhancing its stability and cell surface expression (PubMed:24075985, PubMed:26344097). Down-regulates the response to bacterial lipopolysaccharide (LPS) via its role in IFNAR1 deubiquitination (PubMed:24075985).

  • 組織特異性

    Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Aberrantly expressed in the vast majority of breast tumors.

  • 関連疾患

    A chromosomal aberration involving BRCC3 is a cause of pro-lymphocytic T-cell leukemia (T-PLL). Translocation t(X;14)(q28;q11) with TCRA.

  • 配列類似性

    Belongs to the peptidase M67A family. BRCC36 subfamily.
    Contains 1 MPN (JAB/Mov34) domain.

  • 細胞内局在

    Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle pole. Localizes at sites of DNA damage at double-strand breaks (DSBs) (PubMed:20656690, PubMed:26344097). Interaction with FAM175B/ABRO1 retains BRCC3 in the cytoplasm (PubMed:20656690).
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • BRCA1-A complex subunit BRCC36 antibody
    • BRCA1/BRCA2 containing complex subunit 3 antibody
    • BRCA1/BRCA2 containing complex subunit 36 antibody
    • BRCA1/BRCA2-containing complex subunit 3 antibody
    • BRCA1/BRCA2-containing complex subunit 36 antibody
    • Brcc3 antibody
    • BRCC3_HUMAN antibody
    • BRCC36 antibody
    • BRISC complex subunit BRCC36 antibody
    • C6.1A antibody
    • Chromosome X open reading frame 53 antibody
    • CXorf53 antibody
    • Lys-63-specific deubiquitinase BRCC36 antibody
    • OTTHUMP00000061450 antibody
    see all

画像

  • Western blot - Anti-BRCC36 antibody [EPR4365] (ab108295)
    Western blot - Anti-BRCC36 antibody [EPR4365] (ab108295)

    Lane 1: Wild type HAP1 whole cell lysate (40 µg)
    Lane 2: Empty Lane
    Lane 3: BRCC36 knockout  HAP1 whole cell lysate (40 µg)
    Lane 4: MCF7 whole cell lysate (40 µg) 

    Lanes 1 - 4: Merged signal (red and green). Green - ab108295 observed at 36 kDa. Red - loading control, ab18058, observed at 130 kDa.

    ab108295 was shown to recognize empty when empty knockout samples were used, along with additional cross-reactive bands. Wild-type and empty knockout samples were subjected to SDS-PAGE.  Ab108295 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-BRCC36 antibody [EPR4365] (ab108295)
    Western blot - Anti-BRCC36 antibody [EPR4365] (ab108295)
    All lanes : Anti-BRCC36 antibody [EPR4365] (ab108295) at 1/1000 dilution

    Lane 1 : MCF7 cell lysate
    Lane 2 : SKBR3 cell lysate
    Lane 3 : 293T cell lysate
    Lane 4 : Human fetal kidney lysate
    Lane 5 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 36 kDa

  • Anti-BRCC36 antibody [EPR4365] (ab108295)
    Anti-BRCC36 antibody [EPR4365] (ab108295)

参考文献 (0)

ab108295 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab108295 は論文での使用が確認できていません。

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