Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (HT29 tumor taken out Balb/C nude mice)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Ph6
Permeabilization
No
Specification
HT29 tumor taken out Balb/C nude mice
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 20°C
Fixative
Formaldehyde
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Antibody Dilution Buffer CST
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: ImmPRESS® HRP Anti-Mouse IgG (Peroxidase) Polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
Brown: BRCA-1 (DAB)
Blue: Nucleus (Hematoxylin)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Mar 20 2020