Application
Western blot
Sample
Human Cell lysate - whole cell (Ovarian Tumor)
Gel Running Conditions
Reduced Denaturing (3-8%)
Loading amount
30 µg
Specification
Ovarian Tumor
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 18°C
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: IRDye 680LT Goat anti-Mouse IgG (H + L)
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye 680LT
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye 680LT
Detection
Detection method
Licor
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 210 kDa Non-specific: 80 kDa
Positive control
PEO1
Additional data
Abcam response
Thank you for your review.
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投稿 May 05 2014