Application
Immunocytochemistry
Sample
Human Cultured Cells (MCF-7,HCC3153,UWB1.289)
Permeabilization
Yes - Methanol
Specification
MCF-7,HCC3153,UWB1.289
Blocking step
VECTASTAIN Elite ABC Kit (Vector laboratories) ) as blocking agent for 30 minute(s) · Concentration: 100%
Fixative
Formaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 26°C · Diluent: PBS
Dilution
1/15
Secondary antibody
Name
Non-Abcam antibody was used: VECTASTAIN Elite ABC Kit (Mouse IgG)
Host species: Horse
Clonality: Polyclonal
Conjugation: Biotin
Host species: Horse
Clonality: Polyclonal
Conjugation: Biotin
Additional data
Additional Notes
In our hands and at the highest dilutions recommended (1/15 and 1/50), the staining of BRCA1 clearly appears in the 3 cell lines. Although slightly lower than in MCF-7 (no mutation of BRCA1), it is present in even cell lines with a mutation of exon 11 (HCC3153 and UWB1.289). We cannot then propose to distinguish sporadic from BRCA1 mutated hereditary cancer cells using this antibody in immunocytochemistry.
Abcam response
We have not yet validated this antibody in immunocytochemistry. This researcher has used our AbTrial program to test this application risk free. We would welcome more feedback on the use of this antibody in this application.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Sophie Doisneau-Sixou
Verified customer
投稿 Oct 17 2015