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  1. Link

    brca1-antibody-ab9141.pdf

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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Ubiquitylation
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Anti-BRCA1 抗体 (ab9141)

  • Datasheet
  • SDS
Reviews (2)Q&A (2)References (5)

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Western blot - Anti-BRCA1 antibody (ab9141)
  • Immunoprecipitation - Anti-BRCA1 antibody (ab9141)

Key features and details

  • Rabbit polyclonal to BRCA1
  • Suitable for: IP, WB
  • Reacts with: Human
  • Isotype: IgG

こちらの製品もご検討ください

二次抗体
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
一次抗体
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Anti-BRCA1 antibody [EPR19433] (ab213929)
一次抗体
Product image
Anti-Rad51 antibody [EPR4030(3)] - BSA and Azide free (ab221796)

関連製品

製品の概要

  • 製品名

    Anti-BRCA1 antibody
    BRCA1 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to BRCA1
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: IP, WBmore details
  • 種交差性

    交差種: Human
    交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • 免疫原

    Synthetic peptide (Human) - which represents aportion within the last 100 amino acids of the human Breast Cancer Gene 1 (GenBank PID 627392), conjugated to KLH.

  • ポジティブ・コントロール

    • WB: HEK-293T whole cell lysate. IP: HEK293-T whole cell lysate.
  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー

    pH: 7
    Preservative: 0.1% Sodium azide
    Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • 特記事項(精製)

    Antibodies were affinity purified using thepeptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nmof 1.4 equals 1.0 mg of IgG.
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Ubiquitylation
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • BRCA1
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ubiquitin E3 Enzymes
    • RING Finger E3 Ligase
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Tumor Suppressors
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • Other

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human BRCA1 protein (ab82204)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab9141の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IP (1)
Use at 2-10 µg/mg of lysate.
WB (1)
1/1000 - 1/10000. Detects a band of approximately 220 kDa (predicted molecular weight: 220 kDa).
特記事項
IP
Use at 2-10 µg/mg of lysate.
WB
1/1000 - 1/10000. Detects a band of approximately 220 kDa (predicted molecular weight: 220 kDa).

ターゲット情報

  • 機能

    E3 ubiquitin-protein ligase that specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and plays a central role in DNA repair by facilitating cellular responses to DNA damage. It is unclear whether it also mediates the formation of other types of polyubiquitin chains. The E3 ubiquitin-protein ligase activity is required for its tumor suppressor function. The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Regulates centrosomal microtubule nucleation. Required for normal cell cycle progression from G2 to mitosis. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Involved in transcriptional regulation of P21 in response to DNA damage. Required for FANCD2 targeting to sites of DNA damage. May function as a transcriptional regulator. Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation. Contributes to homologous recombination repair (HRR) via its direct interaction with PALB2, fine-tunes recombinational repair partly through its modulatory role in the PALB2-dependent loading of BRCA2-RAD51 repair machinery at DNA breaks.
  • 組織特異性

    Isoform 1 and isoform 3 are widely expressed. Isoform 3 is reduced or absent in several breast and ovarian cancer cell lines.
  • パスウェイ

    Protein modification; protein ubiquitination.
  • 関連疾患

    Defects in BRCA1 are a cause of susceptibility to breast cancer (BC) [MIM:114480]. A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case. Note=Mutations in BRCA1 are thought to be responsible for 45% of inherited breast cancer. Moreover, BRCA1 carriers have a 4-fold increased risk of colon cancer, whereas male carriers face a 3-fold increased risk of prostate cancer. Cells lacking BRCA1 show defects in DNA repair by homologous recombination.
    Defects in BRCA1 are a cause of susceptibility to breast-ovarian cancer familial type 1 (BROVCA1) [MIM:604370]. A condition associated with familial predisposition to cancer of the breast and ovaries. Characteristic features in affected families are an early age of onset of breast cancer (often before age 50), increased chance of bilateral cancers (cancer that develop in both breasts, or both ovaries, independently), frequent occurrence of breast cancer among men, increased incidence of tumors of other specific organs, such as the prostate. Note=Mutations in BRCA1 are thought to be responsible for more than 80% of inherited breast-ovarian cancer.
    Defects in BRCA1 are a cause of genetic susceptibility to ovarian cancer [MIM:113705].
  • 配列類似性

    Contains 2 BRCT domains.
    Contains 1 RING-type zinc finger.
  • ドメイン

    The BRCT domains recognize and bind phosphorylated pSXXF motif on proteins. The interaction with the phosphorylated pSXXF motif of FAM175A/Abraxas, recruits BRCA1 at DNA damage sites.
    The RING-type zinc finger domain interacts with BAP1.
  • 翻訳後修飾

    Phosphorylation at Ser-308 by STK6/AURKA is required for normal cell cycle progression from G2 to mitosis. Phosphorylated in response to IR, UV, and various stimuli that cause checkpoint activation, probably by ATM or ATR.
    Autoubiquitinated, undergoes 'Lys-6'-linked polyubiquitination. 'Lys-6'-linked polyubiquitination does not promote degradation.
  • 細胞内局在

    Cytoplasm; Nucleus. Localizes at sites of DNA damage at double-strand breaks (DSBs) and recruitment to DNA damage sites is mediated by the BRCA1-A complex.
  • Target information above from: UniProt accession P38398 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 672 Human
    • Entrez Gene: 712634 Rhesus monkey
    • Omim: 113705 Human
    • SwissProt: Q9GKK8 Chimpanzee
    • SwissProt: Q6J6I8 Gorilla
    • SwissProt: P38398 Human
    • SwissProt: Q6J6I9 Rhesus monkey
    • Unigene: 194143 Human
    • 別名

      • BRCA 1 antibody
      • BRCA1 antibody
      • BRCA1 DNA repair associated antibody
      • BRCA1/BRCA2 containing complex subunit 1 antibody
      • BRCA1/BRCA2-containing complex, subunit 1 antibody
      • BRCA1_HUMAN antibody
      • BRCAI antibody
      • BRCC 1 antibody
      • BRCC1 antibody
      • Breast and ovarian cancer susceptibility protein 1 antibody
      • Breast Cancer 1 antibody
      • Breast Cancer 1 Early Onset antibody
      • Breast cancer type 1 susceptibility protein antibody
      • BROVCA1 antibody
      • FANCS antibody
      • IRIS antibody
      • PNCA4 antibody
      • PPP1R53 antibody
      • Protein phosphatase 1 regulatory subunit 53 antibody
      • PSCP antibody
      • RING finger protein 53 antibody
      • RNF53 antibody
      see all

    画像

    • Western blot - Anti-BRCA1 antibody (ab9141)
      Western blot - Anti-BRCA1 antibody (ab9141)
      Anti-BRCA1 antibody (ab9141) at 0.04 µg/ml + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer) at 50 µg

      Predicted band size: 220 kDa


      Exposure time: 30 seconds
    • Immunoprecipitation - Anti-BRCA1 antibody (ab9141)
      Immunoprecipitation - Anti-BRCA1 antibody (ab9141)

      Detection of BRCA1 by immunoprecipitation of whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer.

      Lane 1: Anti-BRCA1 antibody (ab9141) used for IP at 6 µg per reaction.

      Lane 2: IgG Control

      For blotting immunoprecipitated BRCA1, ab9141 was used at 0.04 µg/mL.

      Detection: Chemiluminescence with an exposure time of 75 seconds.

    プロトコール

    • Western blot protocols

    Click here to view the general protocols

    データシートおよび資料

    • SDS download

    • Datasheet download

      Download

    参考文献 (5)

    ab9141 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab9141 は 5 報の論文で使用されています。

    • Al-Yousef N  et al. Curcumin induces re-expression of BRCA1 and suppression of ? synuclein by modulating DNA promoter methylation in breast cancer cell lines. Oncol Rep 43:827-838 (2020). PubMed: 32020216
    • Li J  et al. CDK9 inhibitor CDKI-73 is synergetic lethal with PARP inhibitor olaparib in BRCA1 wide-type ovarian cancer. Am J Cancer Res 10:1140-1155 (2020). PubMed: 32368391
    • Lv W  et al. KIAA0101 inhibition suppresses cell proliferation and cell cycle progression by promoting the interaction between p53 and Sp1 in breast cancer. Biochem Biophys Res Commun 503:600-606 (2018). PubMed: 29902451
    • Xu J  et al. Estrogen receptor-a promoter methylation is a biomarker for outcome prediction of cisplatin resistance in triple-negative breast cancer. Oncol Lett 15:2855-2862 (2018). PubMed: 29456719
    • Lixia M  et al. Alternative splicing of breast cancer associated gene BRCA1 from breast cancer cell line. J Biochem Mol Biol 40:15-21 (2007). WB ; Human . PubMed: 17244477

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-4 of 4 Abreviews or Q&A

    Western blot abreview for Anti-BRCA1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa cells and mouse adult testis)
    Loading amount
    40 µg
    Specification
    HeLa cells and mouse adult testis
    Gel Running Conditions
    Reduced Denaturing (8)
    Read More

    Abcam user community

    Verified customer

    投稿 Apr 21 2009

    Immunoprecipitation abreview for Anti-BRCA1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - nuclear (osteosarcoma (u2os cells))
    Total protein in input
    40 µg
    Specification
    osteosarcoma (u2os cells)
    Immuno-precipitation step
    Protein A
    Read More

    Abcam user community

    Verified customer

    投稿 Mar 06 2007

    Question

    We bought anti-BRCA anobody(ab9141). Do you know the size of the BRCA detected by this polyclonal Ab?

    Read More

    Abcam community

    Verified customer

    Asked on Feb 25 2003

    Answer

    As stated on the datasheet, this antibody (ab9141 -- Rabbit polyclonal to BRCA 1) is expected to detect a band in excess of 220 kDa.

    Read More

    Abcam Scientific Support

    Answered on Mar 03 2003

    Question

    Do you have a western picture, I could see?

    Read More

    Abcam community

    Verified customer

    Asked on Mar 19 2002

    Answer

    No, we do not have a blot image for this antibody.

    Read More

    Abcam Scientific Support

    Answered on Mar 19 2002

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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