Anti-BPOZ/ABTB1 抗体 (ab1077)
製品の概要
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製品名
Anti-BPOZ/ABTB1 antibody -
製品の詳細
Goat polyclonal to BPOZ/ABTB1 -
由来種
Goat -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide corresponding to Human BPOZ/ABTB1 aa 323-336 (N terminal).
Sequence:RALEDLLVSIGLDC
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ポジティブ・コントロール
- Heart lysate.
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特記事項
This product was previously labelled as BPOZ
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Tris saline, 0.02% Na azide, pH7.3 with 0.5% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
Our Abpromise guarantee covers the use of ab1077 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | Use at an assay dependent concentration. PubMed: 24076025 | |
WB | Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa). |
ターゲット情報
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機能
May act as a mediator of the PTEN growth-suppressive signaling pathway. May play a role in developmental processes. -
組織特異性
Ubiquitously expressed in all fetal tissues examined including heart, brain, liver, and kidney. Also expressed at lower levels in both adult heart and hypertrophic heart. -
配列類似性
Contains 2 ANK repeats.
Contains 2 BTB (POZ) domains. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 80325 Human
- Entrez Gene: 80283 Mouse
- Omim: 608308 Human
- SwissProt: Q969K4 Human
- SwissProt: Q99LJ2 Mouse
- Unigene: 107812 Human
- Unigene: 166858 Mouse
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別名
- ABTB 1 antibody
- Abtb1 antibody
- ABTB1_HUMAN antibody
see all
画像
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ab1077 at a concentration of 0.2µg/ml staining approximately 40kDa BPOZ/ABTB1 in human brain extract (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
ab1077 at a concentration of 0.2µg/ml staining approximately 40kDa BPOZ/ABTB1 in human brain extract (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
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Immunocytochemistry/ Immunofluorescence - Anti-BPOZ/ABTB1 antibody (ab1077)Image from Roy A et al., FEBS Lett. 2013;587(21):3567-74. Fig 2(A).; doi: 10.1016/j.febslet.2013.09.020 with permission from Elsevier.
Immunocytochemical/Immunofluorescence analysis of mouse primary neurons stimulated with 5 μM of MPP+ for 0, 6, 12, and 24 hours labelling BPOZ/ABTB1 (green), TH (red) and Nuclei (DAPI - blue).
Coverslips containing neurons cultured to 70–80% confluence were fixed with chilled methanol overnight, followed by two rinses with PBS. Samples were blocked with 2% BSA in PBS containing Tween 20 and Triton X-100 for 30 minutes and incubated at room temperature under shaking conditions for 6 hours in PBS containing the ab1077 (1:200), TH (1:1000). After four 15 minute washes in PBS, slides were incubated with Cy2- or Cy5-labelled secondary antibodies (1:200) for 1 hour under shaking conditions. Following four 15 minute washes with PBS, cells were incubated for 4–5 minutes with DAPI (1:10000).
データシートおよび資料
参考文献
This product has been referenced in:
- Roy A & Pahan K Ankyrin repeat and BTB/POZ domain containing protein-2 inhibits the aggregation of alpha-synuclein: Implications for Parkinson's disease. FEBS Lett 587:3567-74 (2013). ICC/IF ; Mouse . Read more (PubMed: 24076025) »