Application
Western blot
Sample
Cow Tissue lysate - whole (Liver)
Gel Running Conditions
Reduced Denaturing (gel 12%)
Loading amount
5 µg
Specification
Liver
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 3% · Temperature: 25°C
Other product details
Incubation time
17 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: over night
Dilution
1/5000
Secondary antibody
Secondary antibody
None used
Detection
Detection method
ECL
Exposure
3 second(s)
Bands
Specific: 69.3 kDa Non-specific: 25, 32 kDa
Positive control
1ug BSA(Albumin bovine serum)
Additional data
Additional Notes
BSA conjugation of HRP antibody – Loading control(ab7637)
Lane 1: 5ug cow liver
Lane 2 : 10ug cow liver
Lane 3 : 20ug cow liver
Lane 4 : 1ug BSA (positive control)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Mar 07 2018