Anti-Bovine Papilloma Virus E2 抗体 [5H4] (ab1072)

Submit an Abreview Q&A (1)

製品の概要

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab1072 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 - 2 µg/ml.
ELISA Use at an assay dependent dilution.
IP Use at an assay dependent dilution.
  • 追加情報
    Is unsuitable for WB.

    • ターゲット情報

    • 関連性
      The bovine papilloma virus E2 protein is the master regulator of papillomavirus replication and transcription. Binds to the E2RE response element (5'-ACCNNNNNNGGT-3') present in multiple copies in the regulatory region. Can either activate or repress transcription depending on E2RE's position with regards to proximal promoter elements. Repression occurs by sterically hindering the assembly of the transcription initiation complex. The E1-E2 complex binds to the origin of DNA replication.
    • 細胞内局在
      Host cell nucleus
    • 別名
      • BPVgp4 antibody
      • E2 antibody
      • Regulatory protein E2 antibody

    画像

    • Immunocytochemistry/ Immunofluorescence - Anti-Bovine Papilloma Virus E2 antibody [5H4] (ab1072)
      Immunocytochemistry/ Immunofluorescence - Anti-Bovine Papilloma Virus E2 antibody [5H4] (ab1072)

      Immunofluorescent staining of U2OS cells expressing BPV E2 protein with ab1072. The cells were counter-stained with DAPI, and the upper shows an image taken with illumination at both 460 nm and 488 nm, whereas the lower image shows illumination at 488 nm.

    参考文献

    ab1072 has not yet been referenced specifically in any publications.

    ab1072 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

    レビューを送る 質問を送る

    1 Review or Q&A

    10
    Votes: Highest First

    BATCH NUMBER -- NOT SPECIFIED -- ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM multiple nonspecific bands with background. no specific bands revealed SAMPLE cell extracts from transient transfected 293T cells. E2-...

    Read More

    I'm sorry to hear you are having problems with ab1072. I am currently trying to find out more information about the protocol used to test the antibody in WB. Have you tested the blot without milk blocking and if the non specific bands could be due ...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    登録