保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
バッファーConstituent: 50% Glycerol
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精製度Protein A purified
- pQ-NTag/B vector (ab1041)
- pQ-Ctag/A vector (ab1051)
- pQM-NTag/C intron vector (ab1067)
- Anti-Bovine Papilloma Virus E2 antibody [5H4] (ab1072)
- Anti-E2 tag antibody [5E11] (ab977)
- Anti-E2 tag antibody [3F12] (ab978)
- Anti-Bovine Papilloma Virus E2 antibody [1E2] (ab979)
- Anti-Bovine Papilloma Virus E2 antibody [1E4] (ab980)
Our Abpromise guarantee covers the use of ab1011 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IF(ICC): 1/1000 in a diluent containing 1% BSA in PBS. Incubate for 1 hour at room temperature.
IP: Use at an assay dependent dilution to isolate E2Tag-tagged proteins and associated proteins/protein binding partners. Use protein G-agarose for binding experiments.
Is unsuitable for WB.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
関連性The bovine papilloma virus E2 protein is the master regulator of papillomavirus replication and transcription. Binds to the E2RE response element (5'-ACCNNNNNNGGT-3') present in multiple copies in the regulatory region. Can either activate or repress transcription depending on E2RE's position with regards to proximal promoter elements. Repression occurs by sterically hindering the assembly of the transcription initiation complex. The E1-E2 complex binds to the origin of DNA replication.
細胞内局在Host cell nucleus
- Entrez Gene: 1489020 Other species
- BPVgp4 antibody
- E2 antibody
- Regulatory protein E2 antibody