Anti-Bovine Papilloma Virus E2 抗体 [1E4] (ab980)
製品の概要
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製品名Anti-Bovine Papilloma Virus E2 antibody [1E4]
Bovine Papilloma Virus E2 一次抗体 製品一覧 -
製品の詳細Mouse monoclonal [1E4] to Bovine Papilloma Virus E2
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由来種Mouse
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アプリケーション適用あり: WB, IP, ICC/IFmore details
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種交差性交差種: Cow
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免疫原
Mapped to amino acids 250-280.
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エピトープNot Determined.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーConstituent: 50% Glycerol
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Concentration information loading... -
精製度Protein A purified
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ポリ/モノモノクローナル
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クローン名1E4
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アイソタイプIgG1
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研究分野
関連製品
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Compatible Secondaries
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Related Products
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- pQ-Ctag/A vector (ab1051)
- pQM-NTag/C intron vector (ab1067)
- Anti-Bovine Papilloma Virus E2 antibody [5H4] (ab1072)
- Anti-E2 tag antibody [5E11] (ab977)
- Anti-E2 tag antibody [3F12] (ab978)
- Anti-Bovine Papilloma Virus E2 antibody [1E2] (ab979)
アプリケーション
Our Abpromise guarantee covers the use of ab980 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | ||
| IP | ||
| ICC/IF |
追加情報
IF (ICC): 1/500- 1/1000 (1hr, RT). Dilute in a diluent containing 1% BSA in PBS.
IP: Use at an assay dependent dilution to isolate E2Tag-tagged proteins and associated proteins/protein binding partners. Use protein G-agarose.
WB: 1/1000 - 1/5000 (1hr, RT). Dilute in a diluent containing 2% non-fat dry milk in TBST.
(Background can be reduced by adding NaCl up to a concentration of 1.5 M.)Incubate
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
IP: Use at an assay dependent dilution to isolate E2Tag-tagged proteins and associated proteins/protein binding partners. Use protein G-agarose.
WB: 1/1000 - 1/5000 (1hr, RT). Dilute in a diluent containing 2% non-fat dry milk in TBST.
(Background can be reduced by adding NaCl up to a concentration of 1.5 M.)Incubate
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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関連性The bovine papilloma virus E2 protein is the master regulator of papillomavirus replication and transcription. Binds to the E2RE response element (5'-ACCNNNNNNGGT-3') present in multiple copies in the regulatory region. Can either activate or repress transcription depending on E2RE's position with regards to proximal promoter elements. Repression occurs by sterically hindering the assembly of the transcription initiation complex. The E1-E2 complex binds to the origin of DNA replication.
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細胞内局在Host cell nucleus
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別名
- BPVgp4 antibody
- E2 antibody
- Regulatory protein E2 antibody
画像
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![Western blot - Anti-Bovine Papilloma Virus E2 antibody [1E4] (ab980)](https://www.abcam.co.jp/ps/products/0/ab980/Images/ab980-172809-ab980wb.jpg)
Western blot - Anti-Bovine Papilloma Virus E2 antibody [1E4] (ab980)All lanes : Anti-Bovine Papilloma Virus E2 antibody [1E4] (ab980)
Lane 1 : Purified recombinant Bovine Papillomavirus E2 protein at 15000 µg
Lane 2 : Purified recombinant Bovine Papillomavirus E2 protein at 10000 µg
Lane 3 : Purified recombinant Bovine Papillomavirus E2 protein at 5000 µg
Lane 4 : Purified recombinant Bovine Papillomavirus E2 protein at 1000 µg
Predicted band size: 45 kDa
プロトコール
参考文献
This product has been referenced in:
- Della Gatta G et al. Reverse engineering of TLX oncogenic transcriptional networks identifies RUNX1 as tumor suppressor in T-ALL. Nat Med 18:436-40 (2012). ChIP . Read more (PubMed: 22366949) »
- Kurg R et al. Effect of bovine papillomavirus E2 protein-specific monoclonal antibodies on papillomavirus DNA replication. J Virol 73:4670-7 (1999). Read more (PubMed: 10233926) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"