Anti-Bovine Papilloma Virus E2 抗体 [1E4] (ab980)
Key features and details
- Mouse monoclonal [1E4] to Bovine Papilloma Virus E2
- Suitable for: WB, IP, ICC/IF
- Reacts with: Cow
- Isotype: IgG1
製品の概要
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製品名
Anti-Bovine Papilloma Virus E2 antibody [1E4]
Bovine Papilloma Virus E2 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [1E4] to Bovine Papilloma Virus E2 -
由来種
Mouse -
アプリケーション
適用あり: WB, IP, ICC/IFmore details -
種交差性
交差種: Cow -
免疫原
Mapped to amino acids 250-280.
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エピトープ
Not Determined.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Constituent: 50% Glycerol -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
1E4 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Related Products
アプリケーション
Our Abpromise guarantee covers the use of ab980 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | ||
| IP | ||
| ICC/IF |
追加情報
IF (ICC): 1/500- 1/1000 (1hr, RT). Dilute in a diluent containing 1% BSA in PBS.
IP: Use at an assay dependent dilution to isolate E2Tag-tagged proteins and associated proteins/protein binding partners. Use protein G-agarose.
WB: 1/1000 - 1/5000 (1hr, RT). Dilute in a diluent containing 2% non-fat dry milk in TBST.
(Background can be reduced by adding NaCl up to a concentration of 1.5 M.)Incubate
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
IP: Use at an assay dependent dilution to isolate E2Tag-tagged proteins and associated proteins/protein binding partners. Use protein G-agarose.
WB: 1/1000 - 1/5000 (1hr, RT). Dilute in a diluent containing 2% non-fat dry milk in TBST.
(Background can be reduced by adding NaCl up to a concentration of 1.5 M.)Incubate
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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関連性
The bovine papilloma virus E2 protein is the master regulator of papillomavirus replication and transcription. Binds to the E2RE response element (5'-ACCNNNNNNGGT-3') present in multiple copies in the regulatory region. Can either activate or repress transcription depending on E2RE's position with regards to proximal promoter elements. Repression occurs by sterically hindering the assembly of the transcription initiation complex. The E1-E2 complex binds to the origin of DNA replication. -
細胞内局在
Host cell nucleus -
別名
- BPVgp4 antibody
- E2 antibody
- Regulatory protein E2 antibody
画像
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![Western blot - Anti-Bovine Papilloma Virus E2 antibody [1E4] (ab980)](https://www.abcam.co.jp/ps/products/0/ab980/Images/ab980-172809-anti-bovine-papilloma-virus-e2-antibody-1e4-western-blot.jpg)
Western blot - Anti-Bovine Papilloma Virus E2 antibody [1E4] (ab980)All lanes : Anti-Bovine Papilloma Virus E2 antibody [1E4] (ab980)
Lane 1 : Purified recombinant Bovine Papillomavirus E2 protein at 15000 µg
Lane 2 : Purified recombinant Bovine Papillomavirus E2 protein at 10000 µg
Lane 3 : Purified recombinant Bovine Papillomavirus E2 protein at 5000 µg
Lane 4 : Purified recombinant Bovine Papillomavirus E2 protein at 1000 µg
Predicted band size: 45 kDa
プロトコール
データシートおよび資料
参考文献
ab980 は 4 報の論文で使用されています。
- Yamauchi T et al. FBXL5 Inactivation in Mouse Brain Induces Aberrant Proliferation of Neural Stem Progenitor Cells. Mol Cell Biol 37:N/A (2017). PubMed: 28069738
- Bocco BM et al. Type 2 Deiodinase Disruption in Astrocytes Results in Anxiety-Depressive-Like Behavior in Male Mice. Endocrinology 157:3682-95 (2016). PubMed: 27501182
- Della Gatta G et al. Reverse engineering of TLX oncogenic transcriptional networks identifies RUNX1 as tumor suppressor in T-ALL. Nat Med 18:436-40 (2012). ChIP . PubMed: 22366949
- Kurg R et al. Effect of bovine papillomavirus E2 protein-specific monoclonal antibodies on papillomavirus DNA replication. J Virol 73:4670-7 (1999). PubMed: 10233926
