Anti-Bovine Papilloma Virus E2 抗体 [1E4] (ab980)

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Key features and details

  • Mouse monoclonal [1E4] to Bovine Papilloma Virus E2
  • Suitable for: WB, IP, ICC/IF
  • Reacts with: Cow
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-Bovine Papilloma Virus E2 antibody [1E4]
    Bovine Papilloma Virus E2 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [1E4] to Bovine Papilloma Virus E2

  • 由来種

    Mouse

  • アプリケーション

    適用あり: WB, IP, ICC/IFmore details

  • 種交差性

    交差種: Cow

  • 免疫原

    Mapped to amino acids 250-280.

  • エピトープ

    Not Determined.

  • 特記事項

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab980 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IP
ICC/IF
  • 追加情報
    IF (ICC): 1/500- 1/1000 (1hr, RT). Dilute in a diluent containing 1% BSA in PBS.
    IP: Use at an assay dependent dilution to isolate E2Tag-tagged proteins and associated proteins/protein binding partners. Use protein G-agarose.
    WB: 1/1000 - 1/5000 (1hr, RT). Dilute in a diluent containing 2% non-fat dry milk in TBST.
    (Background can be reduced by adding NaCl up to a concentration of 1.5 M.)Incubate
    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • ターゲット情報

    • 関連性

      The bovine papilloma virus E2 protein is the master regulator of papillomavirus replication and transcription. Binds to the E2RE response element (5'-ACCNNNNNNGGT-3') present in multiple copies in the regulatory region. Can either activate or repress transcription depending on E2RE's position with regards to proximal promoter elements. Repression occurs by sterically hindering the assembly of the transcription initiation complex. The E1-E2 complex binds to the origin of DNA replication.

    • 細胞内局在

      Host cell nucleus

    • 別名

      • BPVgp4 antibody
      • E2 antibody
      • Regulatory protein E2 antibody

    画像

    • Western blot - Anti-Bovine Papilloma Virus E2 antibody [1E4] (ab980)
      Western blot - Anti-Bovine Papilloma Virus E2 antibody [1E4] (ab980)
      All lanes : Anti-Bovine Papilloma Virus E2 antibody [1E4] (ab980)

      Lane 1 : Purified recombinant Bovine Papillomavirus E2 protein at 15000 µg
      Lane 2 : Purified recombinant Bovine Papillomavirus E2 protein at 10000 µg
      Lane 3 : Purified recombinant Bovine Papillomavirus E2 protein at 5000 µg
      Lane 4 : Purified recombinant Bovine Papillomavirus E2 protein at 1000 µg

      Predicted band size: 45 kDa

    参考文献 (4)

    ab980 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab980 は 4 報の論文で使用されています。

    • Yamauchi T  et al. FBXL5 Inactivation in Mouse Brain Induces Aberrant Proliferation of Neural Stem Progenitor Cells. Mol Cell Biol 37:N/A (2017). PubMed: 28069738
    • Bocco BM  et al. Type 2 Deiodinase Disruption in Astrocytes Results in Anxiety-Depressive-Like Behavior in Male Mice. Endocrinology 157:3682-95 (2016). PubMed: 27501182
    • Della Gatta G  et al. Reverse engineering of TLX oncogenic transcriptional networks identifies RUNX1 as tumor suppressor in T-ALL. Nat Med 18:436-40 (2012). ChIP . PubMed: 22366949
    • Kurg R  et al. Effect of bovine papillomavirus E2 protein-specific monoclonal antibodies on papillomavirus DNA replication. J Virol 73:4670-7 (1999). PubMed: 10233926

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