リコンビナントRabMAb

Anti-BORIS 抗体 [EP12204] - BSA and Azide free (ab269929)

Submit a review Submit a question
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BORIS antibody [EP12204] - BSA and Azide free (ab269929)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BORIS antibody [EP12204] - BSA and Azide free (ab269929)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BORIS antibody [EP12204] - BSA and Azide free (ab269929)
  • Anti-BORIS antibody [EP12204] - BSA and Azide free (ab269929)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP12204] to BORIS - BSA and Azide free
  • Suitable for: IHC-P
  • Reacts with: Mouse, Rat, Human

製品の概要

  • 製品名

    Anti-BORIS antibody [EP12204] - BSA and Azide free
    BORIS 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EP12204] to BORIS - BSA and Azide free

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    See all applications and species data

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • IHC-P: Human, mouse and rat testis tissue.

  • 特記事項

    ab269929 is the carrier-free version of ab187163. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab269929 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab269929 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
IHC-P
Human
アプリケーション Abreviews 特記事項
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
特記事項
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    Testis-specific DNA binding protein responsible for insulator function, nuclear architecture and transcriptional control, which probably acts by recruiting epigenetic chromatin modifiers. Plays a key role in gene imprinting in male germline, by participating in the establishment of differential methylation at the IGF2/H19 imprinted control region (ICR). Directly binds the unmethylated H19 ICR and recruits the PRMT7 methyltransferase, leading to methylate histone H4 'Arg-3' to form H4R3sme2. This probably leads to recruit de novo DNA methyltransferases at these sites (By similarity). Seems to act as tumor suppressor. In association with DNMT1 and DNMT3B, involved in activation of BAG1 gene expression by binding to its promoter. Required for dimethylation of H3 lysine 4 (H3K4me2) of MYC and BRCA1 promoters.

  • 組織特異性

    Testis specific. Specifically expressed in primary spermatocytes.

  • 配列類似性

    Belongs to the CTCF zinc-finger protein family.
    Contains 11 C2H2-type zinc fingers.

  • 発生段階

    Is up-regulated in primary spermatocytes (expression overlapping with the genome-wide erasure of methylation) to become silenced on activation of CTCF in post-meiotic germline cells (expression corresponding to DNA remethylation).

  • 細胞内局在

    Cytoplasm. Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • BORIS like protein antibody
      • Brother of the regulator of imprinted sites antibody
      • Cancer/testis antigen 27 antibody
      • CCCTC binding factor (zinc finger protein) like antibody
      • CCCTC-binding factor antibody
      • CT27 antibody
      • CTCF paralog antibody
      • CTCF T antibody
      • CTCF-like protein antibody
      • Ctcfl antibody
      • CTCFL_HUMAN antibody
      • dJ579F20.2 antibody
      • HMG 1L1 antibody
      • HMGB1L1 antibody
      • MGC163358 antibody
      • MGC169105 antibody
      • MGC169106 antibody
      • Putative high mobility group protein 1 like 1 antibody
      • Putative high mobility group protein B1 like 1 antibody
      • Transcriptional repressor CTCFL antibody
      • Zinc finger protein CTCF-T antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BORIS antibody [EP12204] - BSA and Azide free (ab269929)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BORIS antibody [EP12204] - BSA and Azide free (ab269929)

      Immunohistochemical analysis of paraffin-embedded human testis tissue labeling BORIS with ab187163 at 1/1000 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counterstained with hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab187163).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BORIS antibody [EP12204] - BSA and Azide free (ab269929)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BORIS antibody [EP12204] - BSA and Azide free (ab269929)

      Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling BORIS with ab187163 at 1/1000 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counterstained with hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab187163).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BORIS antibody [EP12204] - BSA and Azide free (ab269929)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BORIS antibody [EP12204] - BSA and Azide free (ab269929)

      Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling BORIS with ab187163 at 1/1000 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counterstained with hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab187163).

    • Anti-BORIS antibody [EP12204] - BSA and Azide free (ab269929)
      Anti-BORIS antibody [EP12204] - BSA and Azide free (ab269929)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab269929 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab269929 は論文での使用が確認できていません。

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    There are currently no Customer reviews or Questions for ab269929.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com