リコンビナントRabMAb

Anti-Bone Sialoprotein 抗体 [EPR23367-50] - BSA and Azide free (ab272320)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bone Sialoprotein antibody [EPR23367-50] - BSA and Azide free (ab272320)
  • Immunohistochemistry (Frozen sections) - Anti-Bone Sialoprotein antibody [EPR23367-50] - BSA and Azide free (ab272320)
  • Immunoprecipitation - Anti-Bone Sialoprotein antibody [EPR23367-50] - BSA and Azide free (ab272320)
  • Anti-Bone Sialoprotein antibody [EPR23367-50] - BSA and Azide free (ab272320)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23367-50] to Bone Sialoprotein - BSA and Azide free
  • Suitable for: IP, IHC-P, IHC-Fr
  • Reacts with: Mouse

製品の概要

  • 製品名

    Anti-Bone Sialoprotein antibody [EPR23367-50] - BSA and Azide free
    Bone Sialoprotein 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR23367-50] to Bone Sialoprotein - BSA and Azide free

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-Fr
    Mouse
    IHC-P
    Mouse
    IP
    Mouse
    See all applications and species data

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • IHC-P: Mouse E14.5 tissue. IHC-Fr: Mouse E14.5 tissue. IP: Mouse fetal bone tissue lysate.

  • 特記事項

    ab272320 is the carrier-free version of ab270457. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

製品の特性

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab272320 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
IHC-Fr
Mouse
IHC-P
Mouse
IP
Mouse
アプリケーション Abreviews 特記事項
IP
Use at an assay dependent concentration.

 

 
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
Use at an assay dependent concentration.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

 
特記事項
IP
Use at an assay dependent concentration.

 

 
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
Use at an assay dependent concentration.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

 
  • 追加情報
    Is unsuitable for WB.

    • ターゲット情報

    • 機能

      Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Promotes Arg-Gly-Asp-dependent cell attachment.

    • 翻訳後修飾

      N-glycosylated; glycans consist of sialated and core-fucosylated bi-, tri- and tetraantennary chains.
      O-glycosylated at eight sites; mucin-type glycans contain Gal, GlcNAc, GalNAc and terminal NeuAc.
      Sulfated on either Tyr-313 or Tyr-314.

    • 細胞内局在

      Secreted.
    • Information by UniProt

    • 参照データベース

      • 別名

        • BNSP antibody
        • Bone sialoprotein 2 antibody
        • Bone sialoprotein II antibody
        • BSP antibody
        • BSP II antibody
        • BSPII antibody
        • Cell binding sialoprotein antibody
        • Cell-binding sialoprotein antibody
        • IBSP antibody
        • Integrin binding sialoprotein antibody
        • Integrin-binding sialoprotein antibody
        • SIAL_HUMAN antibody
        • SPII antibody
        see all

      画像

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bone Sialoprotein antibody [EPR23367-50] - BSA and Azide free (ab272320)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bone Sialoprotein antibody [EPR23367-50] - BSA and Azide free (ab272320)

        Immunohistochemical analysis of paraffin-embedded Mouse E14.5 tissue labeling Bone Sialoprotein with ab270457 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on bones of mouse E14.5 (PMID: 24927920). The section was incubated with ab270457 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

        Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270457).

      • Immunohistochemistry (Frozen sections) - Anti-Bone Sialoprotein antibody [EPR23367-50] - BSA and Azide free (ab272320)
        Immunohistochemistry (Frozen sections) - Anti-Bone Sialoprotein antibody [EPR23367-50] - BSA and Azide free (ab272320)

        Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-1-- permeabilized mouse E14.5 embryo tissue labeling Bone Sialoprotein with ab270457 at 1/500 dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (Green). Positive staining on mouse embryonic bone. The nuclear counter stain is DAPI (Blue).

        Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

        Secondary antibody only control: Secondary antibody is b150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270457).

      • Immunoprecipitation - Anti-Bone Sialoprotein antibody [EPR23367-50] - BSA and Azide free (ab272320)
        Immunoprecipitation - Anti-Bone Sialoprotein antibody [EPR23367-50] - BSA and Azide free (ab272320)

        Bone Sialoprotein was immunoprecipitated from 0.35 mg Mouse fetal bone tissue lysate using ab270457 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab270457 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

        Lane 1: Mouse fetal bone tissue lysate 10ug

        Lane 2: ab270457 IP in Mouse fetal bone tissue lysate

        Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab270457 in Mouse fetal bone tissue lysate

        Blocking and dilution buffer and concentration: 5% NFDM/TBST.

        Exposure time: 41 sseconds.

        The molecular weight observed is consistent with literature (PMID: 14647232)

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270457).

      • Anti-Bone Sialoprotein antibody [EPR23367-50] - BSA and Azide free (ab272320)
        Anti-Bone Sialoprotein antibody [EPR23367-50] - BSA and Azide free (ab272320)

      プロトコール

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      参考文献 (0)

      ab272320 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab272320 は論文での使用が確認できていません。

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