製品の概要

  • 製品名
    Anti-Bone Sialoprotein antibody
    Bone Sialoprotein 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Bone Sialoprotein
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: ELISA, RIA, WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Rat, Human
  • 免疫原

    Human Bone Sialoprotein (bone extract)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab52128 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.
RIA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 35 kDa.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/100.

ターゲット情報

  • 機能
    Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Promotes Arg-Gly-Asp-dependent cell attachment.
  • 翻訳後修飾
    N-glycosylated; glycans consist of sialated and core-fucosylated bi-, tri- and tetraantennary chains.
    O-glycosylated at eight sites; mucin-type glycans contain Gal, GlcNAc, GalNAc and terminal NeuAc.
    Sulfated on either Tyr-313 or Tyr-314.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BNSP antibody
    • Bone sialoprotein 2 antibody
    • Bone sialoprotein II antibody
    • BSP antibody
    • BSP II antibody
    • BSPII antibody
    • Cell binding sialoprotein antibody
    • Cell-binding sialoprotein antibody
    • IBSP antibody
    • Integrin binding sialoprotein antibody
    • Integrin-binding sialoprotein antibody
    • SIAL_HUMAN antibody
    • SPII antibody
    see all

画像

  • ab52128 (2ug/ml) staining Bone Sialoprotein in human bone using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of Bone sialoprotein in sectretory compartments in the cells of the perichondrium
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

参考文献

This product has been referenced in:
  • Bianco D  et al. Alterations of Subchondral Bone Progenitor Cells in Human Knee and Hip Osteoarthritis Lead to a Bone Sclerosis Phenotype. Int J Mol Sci 19:N/A (2018). Read more (PubMed: 29415458) »
  • Liang D  et al. Effects of nicotine on the metabolism and gene expression profile of Sprague-Dawley rat primary osteoblasts. Mol Med Rep 17:8269-8281 (2018). Read more (PubMed: 29658611) »

See all 8 Publications for this product

レビューと Q&A

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Endochondral bone)
Specification
Endochondral bone
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 19°C
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投稿 Jan 25 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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