Anti-BOG 抗体 [EPR9905] (ab157202)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9905] to BOG
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-BOG antibody [EPR9905]
BOG 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR9905] to BOG -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
適用なし: IP -
種交差性
交差種: Human
交差が予測される動物種: Rat -
免疫原
Synthetic peptide within Human BOG. The exact sequence is proprietary.
Database link: O75884 -
ポジティブ・コントロール
- HepG2, MCF7, and HeLa cell lysates; kidney tissue; MCF7 and HeLa cells.
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20ºC. -
バッファー
pH: 7.2
Constituents: 0.35% Sodium citrate, 0.17% Sodium chloride, 0.03% EDTA, 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR9905 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab157202の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/1000 - 1/10000. Predicted molecular weight: 21 kDa.
|
|
IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
ICC/IF |
1/250 - 1/500.
|
特記事項 |
---|
Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 21 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/250 - 1/500. |
ターゲット情報
-
機能
May play a role in the transformation process due to its capacity to confer resistance to the growth-inhibitory effects of TGF-beta1 through interaction with retinoblastoma and the subsequent displacement of E2F-1. -
組織特異性
Widely expressed. Expressed at higher levels in tumor tissues than in normal tissues. -
配列類似性
Belongs to the RBBP9 family. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 10741 Human
- Entrez Gene: 29459 Rat
- Omim: 602908 Human
- SwissProt: O75884 Human
- SwissProt: O88350 Rat
- Unigene: 69330 Human
- Unigene: 162693 Rat
-
別名
- B5T overexpressed gene protein antibody
- B5T-overexpressed gene protein antibody
- BOG antibody
see all
画像
-
All lanes : Anti-BOG antibody [EPR9905] (ab157202) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : MCF-7 cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 21 kDa -
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue, labeling BOG using ab157202 at a 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunofluorescence analysis of MCF7 cells, labeling BOG using ab157202 at a 1/250 dilution.
-
Intracellular Flow Cytometry analysis of permeabilized HeLa cells labeling BOG (red), using ab157202 at a 1/100 dilution, and negative control cells probed with a Rabbit IgG (green)
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab157202 は論文での使用が確認できていません。