Anti-BOB1 抗体 (ab3426)

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Key features and details

  • Goat polyclonal to BOB1
  • Suitable for: WB
  • Reacts with: Mouse
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-BOB1 antibody
    BOB1 一次抗体 製品一覧

  • 製品の詳細

    Goat polyclonal to BOB1

  • 由来種

    Goat

  • アプリケーション

    適用あり: WBmore details

  • 種交差性

    交差種: Mouse
    交差が予測される動物種: Human

  • 免疫原

    Synthetic peptide: DAYALNHTLSVEGF, corresponding to C terminal amino acids 243-256 of Human BOB1.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • Mouse spleen lysate.

  • 特記事項

    Principal Names – POU2AF1; BOB1; OBF1; OCAB; OBF-1; POU domain, class 2, associating factor 1. Official Gene Symbol - POU2AF1. GenBank Accession Number – NP_006226. LocusLink ID - 5450 (human); 18985 (mouse). Gene Ontology terms - transcription cofactor activity; transcription co-activator activity; transcription activating factor; humoral immune response; transcription from Pol II promoter.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

  • バッファー

    pH: 7.30
    Constituents: 3% Sodium deoxycholate, 3% Triton-X-100, 0.3% Tris HCl, 15% Sodium chloride
  • Concentration information loading...

  • 特記事項（精製）

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab3426 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa).

A 1 hour primary incubation is recommended for this product. Detects a band of approximately 30 kDa in Mouse Spleen lysate.

ターゲット情報

  • 機能

    Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.

  • 組織特異性

    B-cell specific.

  • 関連疾患

    Note=A chromosomal aberration involving POU2AF1/OBF1 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with BCL6.

  • 配列類似性

    Belongs to the POU2AF1 family.

  • 翻訳後修飾

    Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • B cell Oct binding protein 1 antibody
      • B cell specific coactivator OBF 1 antibody
      • B cell specific coactivator OBF1 antibody
      • B-cell-specific coactivator OBF-1 antibody
      • BOB 1 antibody
      • BOB-1 antibody
      • OBF 1 antibody
      • OBF1 antibody
      • OBF1_HUMAN antibody
      • OCA B antibody
      • OCA-B antibody
      • OCAB antibody
      • OCT binding factor 1 antibody
      • OCT-binding factor 1 antibody
      • POU class 2 associating factor 1 antibody
      • POU domain class 2 associating factor 1 antibody
      • POU domain class 2-associating factor 1 antibody
      • Pou2af1 antibody
      see all

    画像

    • Western blot - Anti-BOB1 antibody (ab3426)
      Western blot - Anti-BOB1 antibody (ab3426)
      ab3426 at a 1µg/ml concentration staining Bob 1 in mouse spleen lysate (RIPA bufffer, 30 µg total protein per lane, 1 hour incubation) by Western blot (ECL). ab3426 at a 1µg/ml concentration staining Bob 1 in mouse spleen lysate (RIPA bufffer, 30 µg total protein per lane, 1 hour incubation) by Western blot (ECL).

    参考文献 (2)

    ab3426 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab3426 は 2 報の論文で使用されています。

    • Russo MV  et al. Distinct myeloid cell subsets promote meningeal remodeling and vascular repair after mild traumatic brain injury. Nat Immunol 19:442-452 (2018). PubMed: 29662169
    • Joza S  et al. Loss of semaphorin-neuropilin-1 signaling causes dysmorphic vascularization reminiscent of alveolar capillary dysplasia. Am J Pathol 181:2003-17 (2012). PubMed: 23063659

    レビューと Q&A

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    Question

    Please kindly advise whether the antibody ab3426 anti BOB 1 can be applied on paraffin sections.

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    Answer

    Thank you for your email. To our knowledge, this antibody has yet to be tested in IHC. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on and in return we will award you 50 points with the Abcam Loyalty Scheme which can be redeemed on a number of rewards. If you have any additional questions, please let us know.

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