Anti-BNP 抗体 [50E1] (ab20984)
Key features and details
- Mouse monoclonal [50E1] to BNP
- Suitable for: ELISA, ICC/IF, WB, Sandwich ELISA
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-BNP antibody [50E1]
BNP 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [50E1] to BNP -
由来種
Mouse -
特異性
ab20984 detects Human BNP and proBNP. -
アプリケーション
適用あり: ELISA, ICC/IF, WB, Sandwich ELISAmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human BNP conjugated to bovine serum albumin.
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エピトープ
ab20984 binds to epitope 26-32. -
特記事項
This product has switched from ascites to TCS on 29th June 2020. Stock up to lot GR3225984 has been generated from ascites.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
50E1 -
ミエローマ
Sp2/0 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab20984の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
|
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| ICC/IF |
Use at an assay dependent concentration. PubMed: 21482747
|
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| WB |
Use at an assay dependent concentration.
|
|
| Sandwich ELISA |
Use at an assay dependent concentration.
Can be used as capture antibody |
| 特記事項 |
|---|
|
ELISA
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. PubMed: 21482747 |
|
WB
Use at an assay dependent concentration. |
|
Sandwich ELISA
Use at an assay dependent concentration. Can be used as capture antibody |
ターゲット情報
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関連性
Brain natriuretic peptide (BNP) circulates in blood as a peptide hormone with natriuretic, vasodilatory and renin inhibitory properties. BNP is secreted predominantly by the left ventricular myocytes in response to volume expansion and pressure overload. BNP belongs to a family of structurally similar peptide hormones, which includes atrial natriuretic peptide (ANP), BNP, C type natriuretic peptide (CNP) and urodilatin. These peptides are characterized by a common 17 amino acid ring structure with a disulfide bond between two cystein residues. This ring structure shows high homology between different natriuretic peptides (eleven of the 17 amino acid residues are homologous in the ring of each of the natriuretic peptides). BNP is a 32 amino acid peptide with disulfide bond between the cysteine residues Cys10 and Cys26. In earlier studies it has been demonstrated that BNP concentration in blood increases with the severity of congestive heart failure. Quantitative measurement of BNP in blood provides an objective indicator of congestive heart failure severity. -
細胞内局在
Secreted -
参照データベース
- Entrez Gene: 4879 Human
- Omim: 600295 Human
- SwissProt: P16860 Human
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別名
- BNP(1-28) antibody
- BNP(1-29) antibody
- BNP(1-30) antibody
see all
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab20984 は 3 報の論文で使用されています。
- Gong Y et al. Improved LFIAs for highly sensitive detection of BNP at point-of-care. Int J Nanomedicine 12:4455-4466 (2017). PubMed: 28670119
- Tae HJ et al. Cardiac remodeling in the mouse model of Marfan syndrome develops into two distinctive phenotypes. Am J Physiol Heart Circ Physiol 310:H290-9 (2016). PubMed: 26566724
- Tonne JM et al. Secretion of glycosylated pro-B-type natriuretic peptide from normal cardiomyocytes. Clin Chem 57:864-73 (2011). WB, ICC/IF ; Human . PubMed: 21482747