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リコンビナントRabMAb

Anti-BNIP3L/NIX 抗体 [EPR4033] (ab109414)

Reviews (1) Submit a question References (9)
Western blot - Anti-BNIP3L/NIX antibody [EPR4033] (ab109414)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNIP3L/NIX antibody [EPR4033] (ab109414)
  • Anti-BNIP3L/NIX antibody [EPR4033] (ab109414)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4033] to BNIP3L/NIX
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-BNIP3L/NIX antibody [EPR4033]
    BNIP3L/NIX 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR4033] to BNIP3L/NIX

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Pmore details
    適用なし: Flow Cyt,ICC or IP

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Raji and K562 cell lysates; Human breast carcinoma tissue

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab109414の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 24 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

antigen retrieval is recommended.
特記事項
WB
1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 24 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

antigen retrieval is recommended.
追加情報
Is unsuitable for Flow Cyt,ICC or IP.

ターゲット情報

  • 機能

    Induces apoptosis. Interacts with viral and cellular anti-apoptosis proteins. Can overcome the suppressors BCL-2 and BCL-XL, although high levels of BCL-XL expression will inhibit apoptosis. May function as a tumor suppressor. Inhibits apoptosis induced by BNIP3.

  • 配列類似性

    Belongs to the NIP3 family.

  • 翻訳後修飾

    Undergoes progressive proteolysis to an 11 kDa C-terminal fragment, which is blocked by the proteasome inhibitor lactacystin.

  • 細胞内局在

    Nucleus envelope. Endoplasmic reticulum. Mitochondrion. Membrane.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • Adenovirus E1B19k binding protein B5 antibody
    • Adenovirus E1B19K-binding protein B5 antibody
    • BCL2/adenovirus E1B 19 kd protein interacting protein 3a antibody
    • BCL2/adenovirus E1B 19 kDa protein interacting protein 3A antibody
    • BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like antibody
    • BCL2/adenovirus E1B 19 kDa protein-interacting protein 3A antibody
    • BCL2/adenovirus E1B 19kDa interacting protein 3 like antibody
    • BNI3L_HUMAN antibody
    • BNIP3a antibody
    • BNIP3H antibody
    • BNIP3L antibody
    • BNIP3L protein antibody
    • NIP3 like protein X antibody
    • NIP3-like protein X antibody
    • NIP3L antibody
    • NIX antibody
    see all

画像

  • Western blot - Anti-BNIP3L/NIX antibody [EPR4033] (ab109414)
    Western blot - Anti-BNIP3L/NIX antibody [EPR4033] (ab109414)
    All lanes : Anti-BNIP3L/NIX antibody [EPR4033] (ab109414) at 1/1000 dilution

    Lane 1 : Raji cell lysate
    Lane 2 : K562 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 24 kDa
    Observed band size: 35 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNIP3L/NIX antibody [EPR4033] (ab109414)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNIP3L/NIX antibody [EPR4033] (ab109414)

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 1/100 ab109414

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-BNIP3L/NIX antibody [EPR4033] (ab109414)
    Anti-BNIP3L/NIX antibody [EPR4033] (ab109414)

参考文献 (9)

ab109414 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab109414 は 9 報の論文で使用されています。

  • Simpson CL  et al. NIX initiates mitochondrial fragmentation via DRP1 to drive epidermal differentiation. Cell Rep 34:108689 (2021). PubMed: 33535046
  • Dowdell AS  et al. The HIF target ATG9A is essential for epithelial barrier function and tight junction biogenesis. Mol Biol Cell 31:2249-2258 (2020). PubMed: 32726170
  • Yang L  et al. Deferoxamine Treatment Combined With Sevoflurane Postconditioning Attenuates Myocardial Ischemia-Reperfusion Injury by Restoring HIF-1/BNIP3-Mediated Mitochondrial Autophagy in GK Rats. Front Pharmacol 11:6 (2020). PubMed: 32140105
  • Zhou Y  et al. Sulforaphane-cysteine inhibited migration and invasion via enhancing mitophagosome fusion to lysosome in human glioblastoma cells. Cell Death Dis 11:819 (2020). PubMed: 33004792
  • Xu D  et al. NIX-mediated mitophagy protects against proteinuria-induced tubular cell apoptosis and renal injury. Am J Physiol Renal Physiol 316:F382-F395 (2019). PubMed: 30207166
  • Parousis A  et al. Contractile activity attenuates autophagy suppression and reverses mitochondrial defects in skeletal muscle cells. Autophagy 14:1886-1897 (2018). PubMed: 30078345
  • Prieto-Domínguez N  et al. Melatonin enhances sorafenib actions in human hepatocarcinoma cells by inhibiting mTORC1/p70S6K/HIF-1a and hypoxia-mediated mitophagy. Oncotarget 8:91402-91414 (2017). PubMed: 29207653
  • Zhang W & Zhang J Dexmedetomidine preconditioning protects against lung injury induced by ischemia-reperfusion through inhibition of autophagy. Exp Ther Med 14:973-980 (2017). PubMed: 28810549
  • Pedanou VE  et al. The histone H3K9 demethylase KDM3A promotes anoikis by transcriptionally activating pro-apoptotic genes BNIP3 and BNIP3L. Elife 5:N/A (2016). PubMed: 27472901

レビューと Q&A

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レビューを送る 質問を送る

Western blot abreview for Anti-BNIP3L/NIX antibody [EPR4033]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Lung)
Gel Running Conditions
Reduced Denaturing (4-20%)
Loading amount
15 µg
Treatment
10^7 CFU S. Aureus peritoneal clot
Specification
Lung
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Alan Chang

Verified customer

投稿 Mar 20 2014

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