リコンビナントRabMAb

Anti-BMP7 抗体 [EPR23143-31] (ab269586)

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Western blot - Anti-BMP7 antibody [EPR23143-31] (ab269586)
  • Immunoprecipitation - Anti-BMP7 antibody [EPR23143-31] (ab269586)
  • Anti-BMP7 antibody [EPR23143-31] (ab269586)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23143-31] to BMP7
  • Suitable for: WB, IP
  • Reacts with: Human

製品の概要

  • 製品名

    Anti-BMP7 antibody [EPR23143-31]
    BMP7 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR23143-31] to BMP7

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: MCF7 whole cell lysate. IP: MCF7 whole cell lysate.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab269586 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
Flow Cyt
Human
ICC
Human
IHC-P
Human
IP
Human
WB
Human
アプリケーション Abreviews 特記事項
WB
1/1000. Predicted molecular weight: 49 kDa.
IP
1/30.
特記事項
WB
1/1000. Predicted molecular weight: 49 kDa.
IP
1/30.
  • 追加情報
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.

    • ターゲット情報

    • 機能

      Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis.

    • 組織特異性

      Expressed in the kidney and bladder. Lower levels seen in the brain.

    • 配列類似性

      Belongs to the TGF-beta family.

    • 発生段階

      Expressed in the developing eye, brain and ear during embryogenesis.

    • 翻訳後修飾

      Several N-termini starting at positions 293, 300, 315 and 316 have been identified by direct sequencing resulting in secretion of different mature forms (PubMed:17977014).

    • 細胞内局在

      Secreted.
    • Information by UniProt

    • 参照データベース

      • 別名

        • BMP 7 antibody
        • BMP-7 antibody
        • Bmp7 antibody
        • BMP7_HUMAN antibody
        • Bone morphogenetic protein 7 antibody
        • Bone morphogenic protein 7 antibody
        • Eptotermin alfa antibody
        • OP 1 antibody
        • OP-1 antibody
        • OP1 antibody
        • Osteogenic protein 1 antibody
        see all

      画像

      • Western blot - Anti-BMP7 antibody [EPR23143-31] (ab269586)
        Western blot - Anti-BMP7 antibody [EPR23143-31] (ab269586)
        All lanes : Anti-BMP7 antibody [EPR23143-31] (ab269586) at 1/1000 dilution

        Lane 1 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate
        Lane 2 : MDA-MB-231 (human breast adenocarcinoma epithelial cell) whole cell lysate

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

        Predicted band size: 49 kDa
        Observed band size: 49 kDa



        Blocking and diluting buffer and concentration: 5% NFDM/TBST

        The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:16419056, 12792780,11807825) 

        Negative control: MDA-MB-231 (PMID: 16419056) 

        This blot was developed using a higher sensitivity ECL substrate.  Exposure time: 3 minutes

      • Immunoprecipitation - Anti-BMP7 antibody [EPR23143-31] (ab269586)
        Immunoprecipitation - Anti-BMP7 antibody [EPR23143-31] (ab269586)

        BMP7 was immunoprecipitated from 0.35 mg MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate 10µg with ab269586 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab269586 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.

        Lane 1: MCF7 whole cell lysate 10µg

        Lane 2: ab269586 IP in MCF7 whole cell lysate

        Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab269586 in MCF7 whole cell lysate

        Blocking and dilution buffer and concentration: 5% NFDM/TBST.

        Exposure time: 3 seconds.

         

      • Anti-BMP7 antibody [EPR23143-31] (ab269586)
        Anti-BMP7 antibody [EPR23143-31] (ab269586)

      プロトコール

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      参考文献 (0)

      ab269586 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab269586 は論文での使用が確認できていません。

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