Application
Western blot
Sample
Chicken Tissue lysate - whole (retina, RPE, choroid)
Loading amount
20 µg
Specification
retina, RPE, choroid
Gel Running Conditions
Reduced Denaturing (NuPAGE 4-12% Bis-Tris Gel)
Blocking step
(agent) for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: StartingBlock T20 [TBS] Pierce
Secondary antibody
Name
Non-Abcam antibody was used: Millipore, AP132P
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Dilution
1/5000
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 30, 40, 60 kDa
Positive control
Mouse brain
Negative control
none
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Yan Zhang
Verified customer
投稿 Feb 28 2012