Anti-BMP6 抗体 [morph-6.1] (ab15640)
![Immunocytochemistry/ Immunofluorescence - Anti-BMP6 antibody [morph-6.1] (ab15640)](https://www.abcam.co.jp/ps/products/15/ab15640/Images/ab15640-23297-anti-bmp6-antibody-morph-61-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-BMP6 antibody [morph-6.1] (ab15640)")
Key features and details
- Mouse monoclonal [morph-6.1] to BMP6
- Suitable for: ICC/IF
- Reacts with: Rat
- Isotype: IgG1
製品の概要
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製品名
Anti-BMP6 antibody [morph-6.1]
BMP6 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [morph-6.1] to BMP6 -
由来種
Mouse -
アプリケーション
適用あり: ICC/IFmore details -
種交差性
交差種: Rat -
免疫原
Synthetic peptide:
MPGLGRRAQWLCWWWGLLCSCCGPPPLRP
conjugated to KLH, corresponding to amino acids 1-29 of Rat BMP6. -
ポジティブ･コントロール
- Positive staining can be observed in embryonic and adult central nervous system. Rat radial glial cells of the developing central nervous system from E11 to E19. In rat peripheral nerves a selective intracellular immunoreactivity can be found in perinuclear region of most Schwann cells which form the myelin sheath. However, some Schwann cells were negative for the antigen.
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特記事項
Pre-incubation of anti BMP6 with amino-terminal peptide 1-29 of BMP6 inhibits binding of the mAb to tissue sections. Pre-incubation with other amino-terminal synthetic peptides of human BMP7, BMP4, BMP3 and BMP2 as well as an irrelevant peptide does not inhibit specific tissue staining.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. -
バッファー
Preservative: 0.05% KATHON™ CG/ICP
Constituents: 0.5% BSA, 99% PBS -
Concentration information loading... -
精製度
Affinity purified -
一次抗体 備考
Pre-incubation of anti BMP6 with amino-terminal peptide 1-29 of BMP6 inhibits binding of the mAb to tissue sections. Pre-incubation with other amino-terminal synthetic peptides of human BMP7, BMP4, BMP3 and BMP2 as well as an irrelevant peptide does not inhibit specific tissue staining. -
ポリ/モノ
モノクローナル -
クローン名
morph-6.1 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab15640の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | (2) |
Use a concentration of 1 µg/ml.
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| 特記事項 |
|---|
|
ICC/IF
Use a concentration of 1 µg/ml. |
ターゲット情報
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機能
Induces cartilage and bone formation. -
配列類似性
Belongs to the TGF-beta family. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 25644 Rat
- SwissProt: Q04906 Rat
- Unigene: 40476 Rat
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別名
- BMP-6 antibody
- Bmp6 antibody
- BMP6_HUMAN antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-BMP6 antibody [morph-6.1] (ab15640)This image is courtesy of an anonymous Abreview
ab15640 staining human U2-OS cells by ICC/IF. Cells were PFA fixed and permeabilized in Triton-X-100 prior to blocking in 2% serum for 1 hour at 20°C. The primary antibody was diluted 1/100 and incubated with the sample for 1 hour at 37°C. The secondary antibody was Alexa Fluor®488 conjugated chicken anti-mouse, diluted 1/1000.
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![Immunocytochemistry/ Immunofluorescence - Anti-BMP6 antibody [morph-6.1] (ab15640)](https://www.abcam.co.jp/ps/products/15/ab15640/Images/ab15640-23295-anti-bmp6-antibody-morph-61-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-BMP6 antibody [morph-6.1] (ab15640)This image is courtesy of Ruma Raha-Chowdhury, University Of Cambridge, United KingdomICC/IF image of ab15640 staining primary rat myoblast cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 5% normal donkey serum / 0.3M glycine in 0.1% PBS-Tritonx100 for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15640, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488donkey anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab15640 は 8 報の論文で使用されています。
- Radhakrishnan K et al. Orphan Nuclear Receptor ERR? Is a Novel Transcriptional Regulator of IL-6 Mediated Hepatic BMP6 Gene Expression in Mice. Int J Mol Sci 21:N/A (2020). PubMed: 32998264
- Besikcioglu HE et al. Determination of the effects of bone marrow derived mesenchymal stem cells and ovarian stromal stem cells on follicular maturation in cyclophosphamide induced ovarian failure in rats. Taiwan J Obstet Gynecol 58:53-59 (2019). PubMed: 30638481
- Malinverno M et al. Endothelial cell clonal expansion in the development of cerebral cavernous malformations. Nat Commun 10:2761 (2019). PubMed: 31235698
- Garrison P et al. Spatial regulation of bone morphogenetic proteins (BMPs) in postnatal articular and growth plate cartilage. PLoS One 12:e0176752 (2017). IHC ; Mouse . PubMed: 28467498
- Díaz PU et al. BMP2, 4 and 6 and BMPR1B are altered from early stages of bovine cystic ovarian disease development. Reproduction 152:333-50 (2016). Cow . PubMed: 27486268
- Villafan-Bernal JR et al. Molecular profiling of a simple rat model of open tibial fractures with hematoma and periosteum disruption. Exp Ther Med 12:3261-3267 (2016). PubMed: 27882147
- Wensman H et al. Diverse bone morphogenetic protein expression profiles and smad pathway activation in different phenotypes of experimental canine mammary tumors. PLoS One 4:e7133 (2009). IHC-P ; Dog . PubMed: 19771160
- Jung Y et al. Hematopoietic stem cells regulate mesenchymal stromal cell induction into osteoblasts thereby participating in the formation of the stem cell niche. Stem Cells 26:2042-51 (2008). IHC-P ; Mouse . PubMed: 18499897