リコンビナントRabMAb

Anti-BMP4 抗体 [EPR6211] - BSA and Azide free (ab271886)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMP4 antibody [EPR6211] - BSA and Azide free (ab271886)
  • Immunoprecipitation - Anti-BMP4 antibody [EPR6211] - BSA and Azide free (ab271886)
  • Flow Cytometry - Anti-BMP4 antibody [EPR6211] - BSA and Azide free (ab271886)
  • Immunocytochemistry/ Immunofluorescence - Anti-BMP4 antibody [EPR6211] - BSA and Azide free (ab271886)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMP4 antibody [EPR6211] - BSA and Azide free (ab271886)
  • OI-RD Scanning - Anti-BMP4 antibody [EPR6211] - BSA and Azide free (ab271886)
  • Anti-BMP4 antibody [EPR6211] - BSA and Azide free (ab271886)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6211] to BMP4 - BSA and Azide free
  • Suitable for: IHC-P, Flow Cyt, ICC/IF, WB, IP
  • Reacts with: Human

製品の概要

  • 製品名

    Anti-BMP4 antibody [EPR6211] - BSA and Azide free
    BMP4 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR6211] to BMP4 - BSA and Azide free

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    See all applications and species data

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • IP: A549 whole cell lysate. IHC-P: Human breast cancer and colon tissue. Flow Cyt: HeLa cells. ICC/IF: A549 cells.

  • 特記事項

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    ab271886 is the carrier-free version of ab124715. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab271886 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
Flow Cyt
Human
ICC/IF
Human
IHC-P
Human
IP
Human
アプリケーション Abreviews 特記事項
IHC-P
Use at an assay dependent concentration.

See IHC antigen retrieval protocols.

 
Flow Cyt
Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
ICC/IF
Use at an assay dependent concentration.

 

 
WB
Use at an assay dependent concentration. Predicted molecular weight: 47 kDa.
IP
Use at an assay dependent concentration.
特記事項
IHC-P
Use at an assay dependent concentration.

See IHC antigen retrieval protocols.

 
Flow Cyt
Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
ICC/IF
Use at an assay dependent concentration.

 

 
WB
Use at an assay dependent concentration. Predicted molecular weight: 47 kDa.
IP
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Induces cartilage and bone formation. Also act in mesoderm induction, tooth development, limb formation and fracture repair. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction.

  • 組織特異性

    Expressed in the lung and lower levels seen in the kidney. Present also in normal and neoplastic prostate tissues, and prostate cancer cell lines.

  • 関連疾患

    Defects in BMP4 are the cause of microphthalmia syndromic type 6 (MCOPS6) [MIM:607932]; also known as microphthalmia and pituitary anomalies or microphthalmia with brain and digit developmental anomalies. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS6 is characterized by microphthalmia/anophthalmia associated with facial, genital, skeletal, neurologic and endocrine anomalies.
    Defects in BMP4 are the cause of non-syndromic orofacial cleft type 11 (OFC11) [MIM:600625]. Non-syndromic orofacial cleft is a common birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two-third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum. OFC11 is an unusual anomaly consisting of a paramedian scar of the upper lip with an appearance suggesting that a typical cleft lip was corrected in utero.

  • 配列類似性

    Belongs to the TGF-beta family.

  • 細胞内局在

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt

  • 参照データベース

    • 別名

      • zgc:100779 antibody
      • BMP 2B antibody
      • BMP 4 antibody
      • BMP-2B antibody
      • BMP-4 antibody
      • BMP2B antibody
      • BMP2B1 antibody
      • BMP4 antibody
      • BMP4_HUMAN antibody
      • Bone morphogenetic protein 2B antibody
      • Bone morphogenetic protein 4 antibody
      • DVR4 antibody
      • MCOPS6 antibody
      • MGC100779 antibody
      • OFC11 antibody
      • zbmp-4 antibody
      • ZYME antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMP4 antibody [EPR6211] - BSA and Azide free (ab271886)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMP4 antibody [EPR6211] - BSA and Azide free (ab271886)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast cancer tissue sections labeling BMP4 with Purified ab124715 at 1:400 dilution (2.44 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124715).

    • Immunoprecipitation - Anti-BMP4 antibody [EPR6211] - BSA and Azide free (ab271886)
      Immunoprecipitation - Anti-BMP4 antibody [EPR6211] - BSA and Azide free (ab271886)

      ab124715 (purified) at 1:60 dilution (2µg) immunoprecipitating BMP4 in A549 whole cell lysate.
      Lane 1 (input): A549 (Human lung carcinoma epithelial cell) whole cell lysate 10µg
      Lane 2 (+): ab124715 & A549 whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab124715 in A549 whole cell lysate
      For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
      Blocking and diluting buffer: 5% NFDM/TBST.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124715).
    • Flow Cytometry - Anti-BMP4 antibody [EPR6211] - BSA and Azide free (ab271886)
      Flow Cytometry - Anti-BMP4 antibody [EPR6211] - BSA and Azide free (ab271886)

      Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling BMP4 with purified ab124715 at 1:120 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124715).

    • Immunocytochemistry/ Immunofluorescence - Anti-BMP4 antibody [EPR6211] - BSA and Azide free (ab271886)
      Immunocytochemistry/ Immunofluorescence - Anti-BMP4 antibody [EPR6211] - BSA and Azide free (ab271886)

      Immunocytochemistry/Immunofluorescence analysis of A549 (human lung carcinoma) cells labelling BMP4 with purified ab124715 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).

      Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124715).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMP4 antibody [EPR6211] - BSA and Azide free (ab271886)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMP4 antibody [EPR6211] - BSA and Azide free (ab271886)

      Unpurified ab124715, at 1/100, staining BMP4 in paraffin embedded Human colon tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124715).
    • OI-RD Scanning - Anti-BMP4 antibody [EPR6211] - BSA and Azide free (ab271886)
      OI-RD Scanning - Anti-BMP4 antibody [EPR6211] - BSA and Azide free (ab271886)

      Produced using unpurified ab124715

      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124715).
    • Anti-BMP4 antibody [EPR6211] - BSA and Azide free (ab271886)
      Anti-BMP4 antibody [EPR6211] - BSA and Azide free (ab271886)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab271886 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab271886 は論文での使用が確認できていません。

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