Application
Western blot
Sample
Human Cell lysate - other (HELA)
Loading amount
20 µg
Specification
HELA
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Secondary antibody
Name
Non-Abcam antibody was used: GOAT ANTI-RABBIT
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 0 kDa Non-specific: 50, 55, 60 kDa
Additional data
Additional Notes
Not sure if Bmf is expressed in HELAs. If it does, the antibody does not recognize the 25Kd protein
Abcam response
Customer obtained a free of charge replacement vial and was also offered the following tips to try with the current and new vial to improve signal/background ratio:
1. Use ab9655 at 1/500
2. Add a blocking agent such as milk to the antibody dilution buffer
1. Use ab9655 at 1/500
2. Add a blocking agent such as milk to the antibody dilution buffer
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Jan 13 2011