Key features and details
- Rabbit polyclonal to Bmf
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
バッファーPreservative: 0.02% Sodium azide
Concentration information loading...
精製度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab9655 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2 µg/ml. Detects a band of approximately 25 kDa.|
|ICC/IF||Use a concentration of 10 µg/ml.|
機能May play a role in apoptosis. Isoform 1 seems to be the main initiator.
組織特異性Isoform 1 is mainly expressed in B-lymphoid cells. Isoform 2 and isoform 3 are mainly expressed in B-CLL and normal B-cells.
配列類似性Belongs to the Bcl-2 family.
- Information by UniProt
- Bcl 2 modifying factor antibody
- Bcl-2-modifying factor antibody
- Bcl2 modifying factor antibody
Immunocytochemistry/Immunofluorescence of HeLa cells labeling Bmf with Anti-Bmf antibody (ab9655) at 5μg/ml.
Western blot analysis of Bmf expression in human HepG2 (lane 1) and 293 (lane 2) cell lysates with anti-Bmf at 2Western blot analysis of Bmf expression in human HepG2 (lane 1) and 293 (lane 2) cell lysates with anti-Bmf at 2 µg /ml.
ab9655 at 10µg/ml staining Bmf in human Hela cells by Immunocytochemistry.
ab9655 は 8 報の論文で使用されています。
- Lin X et al. lncRNA-ES3/miR-34c-5p/BMF axis is involved in regulating high-glucose-induced calcification/senescence of VSMCs. Aging (Albany NY) 11:523-535 (2019). PubMed: 30654331
- Kabir S et al. The CUL5 ubiquitin ligase complex mediates resistance to CDK9 and MCL1 inhibitors in lung cancer cells. Elife 8:N/A (2019). PubMed: 31294695
- Hayakawa K et al. Early generated B1 B cells with restricted BCRs become chronic lymphocytic leukemia with continued c-Myc and low Bmf expression. J Exp Med 213:3007-3024 (2016). PubMed: 27899442
- Song S et al. The inhibition of histone deacetylase 8 suppresses proliferation and inhibits apoptosis in gastric adenocarcinoma. Int J Oncol 47:1819-28 (2015). PubMed: 26412386
- He XX et al. Bioinformatics analysis identifies miR-221 as a core regulator in hepatocellular carcinoma and its silencing suppresses tumor properties. Oncol Rep 32:1200-10 (2014). PubMed: 24993451
- Blatt NB et al. Bz-423 superoxide signals B cell apoptosis via Mcl-1, Bak, and Bax. Biochem Pharmacol 78:966-73 (2009). WB ; Human . PubMed: 19481066
- Show MD et al. Phosphorylation of mitogen-activated protein kinase 8 (MAPK8) is associated with germ cell apoptosis and redistribution of the Bcl2-modifying factor (BMF). J Androl 29:338-44 (2008). PubMed: 18222916
- Show MD et al. Testicular expression and distribution of the rat bcl2 modifying factor in response to reduced intratesticular testosterone. Biol Reprod 70:1153-61 (2004). IHC-P ; Rat . PubMed: 14668207