Anti-Blooms Syndrome Protein Blm 抗体 [BFL-103] (ab5409)
Key features and details
- Mouse monoclonal [BFL-103] to Blooms Syndrome Protein Blm
- Suitable for: WB, IHC-Fr
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-Blooms Syndrome Protein Blm antibody [BFL-103]
Blooms Syndrome Protein Blm 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [BFL-103] to Blooms Syndrome Protein Blm -
由来種
Mouse -
アプリケーション
適用あり: WB, IHC-Frmore details
適用なし: ICC/IF -
種交差性
交差種: Human -
免疫原
Recombinant full length protein (Human).
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Constituent: PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
BFL-103 -
ミエローマ
NS1 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
Our Abpromise guarantee covers the use of ab5409 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use at an assay dependent concentration. Detects a band of approximately 190 kDa (predicted molecular weight: 160 kDa). | |
| IHC-Fr | Use at an assay dependent concentration. |
追加情報
Is unsuitable for ICC/IF.
ターゲット情報
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機能
Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. -
関連疾患
Defects in BLM are the cause of Bloom syndrome (BLM) [MIM:210900]. BLM is an autosomal recessive disorder characterized by proportionate pre- and postnatal growth deficiency, sun-sensitive telangiectatic hypo- and hyperpigmented skin, predisposition to malignancy, and chromosomal instability. -
配列類似性
Belongs to the helicase family. RecQ subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 HRDC domain. -
翻訳後修飾
Phosphorylated in response to DNA damage. Phosphorylation requires the FANCA-FANCC-FANCE-FANCF-FANCG protein complex, as well as the presence of RMI1. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 641 Human
- Omim: 604610 Human
- SwissProt: P54132 Human
- Unigene: 725208 Human
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別名
- Blm antibody
- BLM_HUMAN antibody
- Bloom syndrome antibody
see all
プロトコール
データシートおよび資料
参考文献 (5)
ab5409 は 5 報の論文で使用されています。
- Chen Y et al. miR-27b-3p and miR-607 cooperatively regulate BLM gene expression by directly targeting the 3'-UTR in PC3 cells. Mol Med Rep 19:4819-4831 (2019). PubMed: 30957187
- Yamauchi T et al. FBXL5 Inactivation in Mouse Brain Induces Aberrant Proliferation of Neural Stem Progenitor Cells. Mol Cell Biol 37:N/A (2017). PubMed: 28069738
- Verdun RE & Karlseder J The DNA damage machinery and homologous recombination pathway act consecutively to protect human telomeres. Cell 127:709-20 (2006). PubMed: 17110331
- Turley H et al. The distribution and expression of the Bloom's syndrome gene product in normal and neoplastic human cells. Br J Cancer 85:261-5 (2001). PubMed: 11461087
- Wu L et al. The Bloom's syndrome gene product interacts with topoisomerase III. J Biol Chem 275:9636-44 (2000). PubMed: 10734115
