Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Non-reduced Denaturing (8% gel)
Sample
Human Cell lysate - whole cell (Colon carcinoma)
Specification
Colon carcinoma
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1 % milk in TBS-Tween
Dilution
1/5000
Secondary antibody
Name
Non-Abcam antibody was used: Rabbit HRP
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/6000
Detection
Detection method
ECL
Bands
Specific: 160 kDa
Exposure
5 minute(s) and 0 second(s)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Eleni Petsalaki
Verified customer
投稿 Oct 08 2012