Anti-Blood Group Lewis y 抗体 [F3] (ab3359)

Reviews (1)Q&A (3)References (20)

Key features and details

  • Mouse monoclonal [F3] to Blood Group Lewis y
  • Suitable for: Flow Cyt, IHC-P, WB
  • Reacts with: Human
  • Isotype: IgM

製品の概要

  • 製品名

    Anti-Blood Group Lewis y antibody [F3]
    Blood Group Lewis y 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [F3] to Blood Group Lewis y

  • 由来種

    Mouse

  • 特異性

    This antibody belongs to the BG-8 group of murine monoclonal antibodies and is specific against the Lewis y (Type 2 Chain) antigen.

  • アプリケーション

    適用あり: Flow Cyt, IHC-P, WBmore details

  • 種交差性

    交差種: Human

  • 免疫原

    SK-LU-3 lung cancer cell line (Human).

  • ポジティブ･コントロール

    • Colon adenocarcinoma (human).

  • 特記事項


    Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis a, Lewis b, Lewis x, Lewis y, and precursor type 1 chain antigens. Lewis y (type 2 chain) antigen is a difucosylated tetrasaccharide found on the Type 2 blood group oligosaccharides of glycolipids and glycoproteins. It is expressed in large bowel tumors and colorectal carcinomas. It may be useful in the classification of human renal and bladder tumors. The Lewis y antigen has been evaluated as a clinical marker for the diagnosis and prognosis of cholangiocarcinoma, hepatocellular carcinoma and breast cancer.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...

  • 精製度

    Ammonium Sulphate Precipitation

  • 一次抗体 備考

    Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis a, Lewis b, Lewis x, Lewis y, and precursor type 1 chain antigens. Lewis y (type 2 chain) antigen is a difucosylated tetrasaccharide found on the Type 2 blood group oligosaccharides of glycolipids and glycoproteins. It is expressed in large bowel tumors and colorectal carcinomas. It may be useful in the classification of human renal and bladder tumors. The Lewis y antigen has been evaluated as a clinical marker for the diagnosis and prognosis of cholangiocarcinoma, hepatocellular carcinoma and breast cancer.

  • ポリ/モノ

    モノクローナル

  • クローン名

    F3

  • アイソタイプ

    IgM

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab3359 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use at an assay dependent concentration.

ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.

 
IHC-P 1/40.
WB Use at an assay dependent concentration.

ターゲット情報

  • 別名

    • Lewis Y blood group antibody

参考文献 (20)

ab3359 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab3359 は 20 報の論文で使用されています。

  • Blanas A  et al. Transcriptional activation of fucosyltransferase (FUT) genes using the CRISPR-dCas9-VPR technology reveals potent N-glycome alterations in colorectal cancer cells. Glycobiology 29:137-150 (2019). PubMed: 30476078
  • Almand EA  et al. Characterization of human norovirus binding to gut-associated bacterial ligands. BMC Res Notes 12:607 (2019). PubMed: 31547886
  • Liu J  et al. Co-expression of Lewis y antigen and CD147 in epithelial ovarian cancer is correlated with malignant progression and poor prognosis. Int J Mol Med 43:1687-1698 (2019). PubMed: 30816446
  • Purohit S  et al. Multiplex glycan bead array for high throughput and high content analyses of glycan binding proteins. Nat Commun 9:258 (2018). PubMed: 29343722
  • Zheng Q  et al. MicroRNA-200c impairs uterine receptivity formation by targeting FUT4 and a1,3-fucosylation. Cell Death Differ 24:2161-2172 (2017). WB . PubMed: 28914881
View all Publications for this product

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Blood Group Lewis y antibody [F3]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
Permeabilization
Yes - 0.05% Tween 20
Specification
Colon
Blocking step
Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Formaldehyde
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Mr. David Ivancic

Verified customer

投稿 Jan 12 2020

Question

Would you kindly indicate the long term storage of the following items:

ab9100

ab3358

ab3359

ab30667

ab86128



We noticed that for example #ab9100 is shipped by 4°C but has to be stored at -20°C !!!

From which day the long term storage applays?



Further more, would you please arrange that this information will be printed on the vials ???!!!

Thank you -

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Answer

Thank you for your inquiry.

I can confirm that the storage temperature of our products can be found on the individual datasheets.

Short term storage is usually two weeks. If the products is not used within two weeks of purchase, we in general recommend to aliquot it and to store as stated for the long term storage. As a general rule, freeze/thaw cycle are to be avoided.

Please find below the long term storage conditions for the products you are interested in:

ab9100 : Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

ab3358 : Store at +4°C.

ab3359 : Store at +4°C.

ab30667 : Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

ab86128 : Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Since the vials are small and we want the text to be readable, we strongly suggest to check the datasheets for the storage information. This will have the positive side effect that also all the other valuable information on the datasheet will be seen.

I hope this information is helpful and wish you a sunny day.

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Question

Dear Sir/Madam; Whil I was searching an antibody, I have a question about one of your antibody. The antibody I am concerning is Anti-Blood Group Lewis y antibody [F3] (ab3359). It is a mouse monoclonal IgM. What I want to know is the concentration of the IgM protein in the tube. I know the product contains 1% BSA but says it is 500ul. For my experiment, I need to know the protein concentration of the IgM.

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Answer

Thank you for contacting us regarding the concentration of AB3359.

Although concentration of this product is lot dependent, our current lot ofab3359 Anti-Blood Group Lewis y antibody [F3] has a concentration of4.5ug/ml.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

We found the expiry date given below was not written on the antibody labels. The customers are querying about this and I would like to have some letter mentioning the expiry dates of these two items. Thank you.

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Answer

Thank you for your enquiry. The antibody storage conditions are detailed on every datasheet under the "Storage instructions" section. We make the general recommend that should our antibodies stipulate storage at -20oC then they can be stored for up to 2 years at this temperature. Likewise we suggest that our antibodies can be stored at -80oC for 10 years. When an antibody can only be kept at 4oC we recommend that it is functional for up to 12 months. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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