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  1. Link

    blood-group-lewis-y-antibody-f3-ab3359.pdf

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Cardiovascular Blood Blood Cell Antigens
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Anti-Blood Group Lewis y 抗体 [F3] (ab3359)

  • Datasheet
  • SDS
Reviews (1)Q&A (3)References (21)

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出荷および受注について

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Key features and details

  • Mouse monoclonal [F3] to Blood Group Lewis y
  • Reacts with: Human
  • Isotype: IgM

製品の概要

  • 製品名

    Anti-Blood Group Lewis y antibody [F3]
    Blood Group Lewis y 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [F3] to Blood Group Lewis y
  • 由来種

    Mouse
  • 特異性

    This antibody belongs to the BG-8 group of murine monoclonal antibodies and is specific against the Lewis y (Type 2 Chain) antigen.
  • 種交差性

    交差種: Human
  • 免疫原

    SK-LU-3 lung cancer cell line (Human).

  • ポジティブ・コントロール

    • Colon adenocarcinoma (human).
  • 特記事項


    Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis a, Lewis b, Lewis x, Lewis y, and precursor type 1 chain antigens. Lewis y (type 2 chain) antigen is a difucosylated tetrasaccharide found on the Type 2 blood group oligosaccharides of glycolipids and glycoproteins. It is expressed in large bowel tumors and colorectal carcinomas. It may be useful in the classification of human renal and bladder tumors. The Lewis y antigen has been evaluated as a clinical marker for the diagnosis and prognosis of cholangiocarcinoma, hepatocellular carcinoma and breast cancer.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • 精製度

    Ammonium Sulphate Precipitation
  • 一次抗体 備考

    Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis a, Lewis b, Lewis x, Lewis y, and precursor type 1 chain antigens. Lewis y (type 2 chain) antigen is a difucosylated tetrasaccharide found on the Type 2 blood group oligosaccharides of glycolipids and glycoproteins. It is expressed in large bowel tumors and colorectal carcinomas. It may be useful in the classification of human renal and bladder tumors. The Lewis y antigen has been evaluated as a clinical marker for the diagnosis and prognosis of cholangiocarcinoma, hepatocellular carcinoma and breast cancer.
  • ポリ/モノ

    モノクローナル
  • クローン名

    F3
  • アイソタイプ

    IgM
  • 研究分野

    • Cardiovascular
    • Blood
    • Blood Cell Antigens
    • Cardiovascular
    • Blood
    • Blood Cell Antigens
    • RBC Antigens
    • Blood Group Antigens

関連製品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Rabbit Anti-Mouse IgG+IgM+IgA H&L (ab8516)
    • Rabbit Anti-Mouse IgG+IgM+IgA H&L (FITC) (ab8517)
    • Goat Anti-Mouse IgM H&L (ab9167)
    • Rabbit Anti-Mouse IgM mu chain (ab9175)
  • Isotype control

    • Mouse IgM [B11/7] - Isotype control (ab91545)

ターゲット情報

  • 別名

    • Lewis Y blood group antibody

プロトコール

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

    • Datasheet
    • SDS
  • 参考文献 (21)

    ab3359 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab3359 は 21 報の論文で使用されています。

    • Li C  et al. Association between the ABO blood group and primary knee osteoarthritis: A case-control study. J Orthop Translat 21:129-135 (2020). PubMed: 32309138
    • Liu J  et al. Co-expression of Lewis y antigen and CD147 in epithelial ovarian cancer is correlated with malignant progression and poor prognosis. Int J Mol Med 43:1687-1698 (2019). PubMed: 30816446
    • Blanas A  et al. Transcriptional activation of fucosyltransferase (FUT) genes using the CRISPR-dCas9-VPR technology reveals potent N-glycome alterations in colorectal cancer cells. Glycobiology 29:137-150 (2019). PubMed: 30476078
    • Almand EA  et al. Characterization of human norovirus binding to gut-associated bacterial ligands. BMC Res Notes 12:607 (2019). PubMed: 31547886
    • Purohit S  et al. Multiplex glycan bead array for high throughput and high content analyses of glycan binding proteins. Nat Commun 9:258 (2018). PubMed: 29343722
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-4 of 4 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Blood Group Lewis y antibody [F3]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Colon)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    Colon
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Mr. David Ivancic

    Verified customer

    投稿 Jan 12 2020

    Question

    Would you kindly indicate the long term storage of the following items:

    ab9100

    ab3358

    ab3359

    ab30667

    ab86128



    We noticed that for example #ab9100 is shipped by 4°C but has to be stored at -20°C !!!

    From which day the long term storage applays?



    Further more, would you please arrange that this information will be printed on the vials ???!!!

    Thank you -

    Read More

    Abcam community

    Verified customer

    Asked on Jul 25 2012

    Answer

    Thank you for your inquiry.

    I can confirm that the storage temperature of our products can be found on the individual datasheets.

    Short term storage is usually two weeks. If the products is not used within two weeks of purchase, we in general recommend to aliquot it and to store as stated for the long term storage. As a general rule, freeze/thaw cycle are to be avoided.

    Please find below the long term storage conditions for the products you are interested in:

    ab9100 : Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    ab3358 : Store at +4°C.

    ab3359 : Store at +4°C.

    ab30667 : Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    ab86128 : Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Since the vials are small and we want the text to be readable, we strongly suggest to check the datasheets for the storage information. This will have the positive side effect that also all the other valuable information on the datasheet will be seen.

    I hope this information is helpful and wish you a sunny day.

    Read More

    Abcam Scientific Support

    Answered on Jul 25 2012

    Question

    Dear Sir/Madam; Whil I was searching an antibody, I have a question about one of your antibody. The antibody I am concerning is Anti-Blood Group Lewis y antibody [F3] (ab3359). It is a mouse monoclonal IgM. What I want to know is the concentration of the IgM protein in the tube. I know the product contains 1% BSA but says it is 500ul. For my experiment, I need to know the protein concentration of the IgM.

    Read More

    Abcam community

    Verified customer

    Asked on Apr 13 2012

    Answer

    Thank you for contacting us regarding the concentration of AB3359.

    Although concentration of this product is lot dependent, our current lot ofab3359 Anti-Blood Group Lewis y antibody [F3] has a concentration of4.5ug/ml.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Apr 13 2012

    Question

    We found the expiry date given below was not written on the antibody labels. The customers are querying about this and I would like to have some letter mentioning the expiry dates of these two items. Thank you.

    Read More

    Abcam community

    Verified customer

    Asked on May 09 2006

    Answer

    Thank you for your enquiry. The antibody storage conditions are detailed on every datasheet under the "Storage instructions" section. We make the general recommend that should our antibodies stipulate storage at -20oC then they can be stored for up to 2 years at this temperature. Likewise we suggest that our antibodies can be stored at -80oC for 10 years. When an antibody can only be kept at 4oC we recommend that it is functional for up to 12 months. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on May 11 2006

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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