Anti-Blood Group Lewis b 抗体 [2-25LE] (ab3968)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Blood Group Lewis b antibody [2-25LE] (ab3968)](https://www.abcam.co.jp/ps/products/3/ab3968/Images/ab3968-455394-AntiBlood-Group-Lewis-b-antibody-Immunohistochemistry-Human-colon-carcinoma.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Blood Group Lewis b antibody [2-25LE] (ab3968)")
Key features and details
- Mouse monoclonal [2-25LE] to Blood Group Lewis b
- Suitable for: WB, ELISA, Flow Cyt, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG1
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Blood Group Lewis b antibody [2-25LE] -
製品の詳細
Mouse monoclonal [2-25LE] to Blood Group Lewis b -
由来種
Mouse -
アプリケーション
適用あり: WB, ELISA, Flow Cyt, ICC/IF, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Full length native protein (purified) corresponding to Human Blood Group Lewis b. Mucin isolated by size exclusion chromatography from an ovarian cyst fluid (pure endocervical type according to the Fenoglio.s classification).
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ポジティブ･コントロール
- IHC: Human colon adenocarcinoma
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Protein A purified -
特記事項（精製）
Purified from hybridoma culture supernatant. Purity >95% by SDS-PAGE. -
ポリ/モノ
モノクローナル -
クローン名
2-25LE -
ミエローマ
unknown -
アイソタイプ
IgG1 -
軽鎖の種類
unknown -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab3968の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use at an assay dependent concentration.
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| ELISA |
Use at an assay dependent concentration.
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| Flow Cyt |
Use at an assay dependent concentration. PubMed: 23144050
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| ICC/IF | (1) |
Use at an assay dependent concentration. PubMed: 17202152
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| IHC-P |
Use a concentration of 5 - 10 µg/ml.
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| 特記事項 |
|---|
|
WB
Use at an assay dependent concentration. |
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ELISA
Use at an assay dependent concentration. |
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Flow Cyt
Use at an assay dependent concentration. PubMed: 23144050 ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
ICC/IF
Use at an assay dependent concentration. PubMed: 17202152 |
|
IHC-P
Use a concentration of 5 - 10 µg/ml. |
ターゲット情報
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関連性
Blood group Lewis b is a carbohydrate determinant carried on both glycolipids and glycoproteins, detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. -
細胞内局在
Plasma membrane - adsorbed onto the surface of erythrocytes. -
参照データベース
- Entrez Gene: 4586 Human
- SwissProt: P98088 Human
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別名
- gastric mucin antibody
- leB antibody
- lewis antigen system antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Blood Group Lewis b antibody [2-25LE] (ab3968)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon adenocarcinoma tissue labelling Blood Group Lewis b with ab3968 at 10 µg/mL.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (16)
ab3968 は 16 報の論文で使用されています。
- Almand EA et al. Characterization of human norovirus binding to gut-associated bacterial ligands. BMC Res Notes 12:607 (2019). PubMed: 31547886
- Purohit S et al. Multiplex glycan bead array for high throughput and high content analyses of glycan binding proteins. Nat Commun 9:258 (2018). PubMed: 29343722
- Cuello HA et al. Aberrant O-glycosylation modulates aggressiveness in neuroblastoma. Oncotarget 9:34176-34188 (2018). PubMed: 30344930
- Zhuang H et al. Overexpression of Lewis y antigen promotes human epididymis protein 4-mediated invasion and metastasis of ovarian cancer cells. Biochimie 105:91-8 (2014). Human . PubMed: 24998328
- Yu Y et al. Human milk contains novel glycans that are potential decoy receptors for neonatal rotaviruses. Mol Cell Proteomics 13:2944-60 (2014). PubMed: 25048705