Anti-Blood Group Lewis b 抗体 [2-25LE] (ab3968)

Reviews (1)Q&A (1)References (16)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Blood Group Lewis b antibody [2-25LE] (ab3968)

    Key features and details

    • Mouse monoclonal [2-25LE] to Blood Group Lewis b
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG1

    リコンビナント抗体で、ロット間での高い再現性を実現

    Product image
    Anti-Mucin 5AC antibody [EPR16904] (ab198294)
    • 異なるロット間での安定した再現性
    • 容易なスケールアップ
    • 評価試験による特異性の確認済み
    • 倫理基準に準拠 - アニマル・フリーの生産

    製品の概要

    • 製品名

      Anti-Blood Group Lewis b antibody [2-25LE]

    • 製品の詳細

      Mouse monoclonal [2-25LE] to Blood Group Lewis b

    • 由来種

      Mouse

    • アプリケーション

      適用あり: IHC-Pmore details

    • 種交差性

      交差種: Human

    • 免疫原

      Full length native protein (purified) corresponding to Human Blood Group Lewis b. Mucin isolated by size exclusion chromatography from an ovarian cyst fluid (pure endocervical type according to the Fenoglio.s classification).

    • ポジティブ･コントロール

      • IHC: Human colon adenocarcinoma

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • バッファー

      pH: 7.40
      Preservative: 0.097% Sodium azide
      Constituent: PBS
    • Concentration information loading...

    • 精製度

      Protein A purified

    • 特記事項（精製）

      Purified from hybridoma culture supernatant. Purity >95% by SDS-PAGE.

    • ポリ/モノ

      モノクローナル

    • クローン名

      2-25LE

    • ミエローマ

      unknown

    • アイソタイプ

      IgG1

    • 軽鎖の種類

      unknown

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab3968の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    IHC-P
    Use a concentration of 5 - 10 µg/ml.
    特記事項
    IHC-P
    Use a concentration of 5 - 10 µg/ml.

    ターゲット情報

    • 関連性

      Blood group Lewis b is a carbohydrate determinant carried on both glycolipids and glycoproteins, detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals.

    • 細胞内局在

      Plasma membrane - adsorbed onto the surface of erythrocytes.

    • 参照データベース

      • 別名

        • gastric mucin antibody
        • leB antibody
        • lewis antigen system antibody
        • lewis b antibody
        • lewis B blood group antigen antibody
        • lewisb antibody
        • major airway glycoprotein antibody
        • MUC5 antibody
        • mucin 5, subtypes A and C, tracheobronchial/gastric antibody
        • mucin 5AC, oligomeric mucus/gel-forming antibody
        • mucin 5AC, oligomeric mucus/gel-forming pseudogene antibody
        • mucin-5 subtype AC, tracheobronchial antibody
        • TBM antibody
        • tracheobronchial mucin antibody
        see all

      画像

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Blood Group Lewis b antibody [2-25LE] (ab3968)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Blood Group Lewis b antibody [2-25LE] (ab3968)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon adenocarcinoma tissue labelling Blood Group Lewis b with ab3968 at 10 µg/mL.

      参考文献 (16)

      ab3968 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab3968 は 16 報の論文で使用されています。

      • Almand EA  et al. Characterization of human norovirus binding to gut-associated bacterial ligands. BMC Res Notes 12:607 (2019). PubMed: 31547886
      • Purohit S  et al. Multiplex glycan bead array for high throughput and high content analyses of glycan binding proteins. Nat Commun 9:258 (2018). PubMed: 29343722
      • Cuello HA  et al. Aberrant O-glycosylation modulates aggressiveness in neuroblastoma. Oncotarget 9:34176-34188 (2018). PubMed: 30344930
      • Zhuang H  et al. Overexpression of Lewis y antigen promotes human epididymis protein 4-mediated invasion and metastasis of ovarian cancer cells. Biochimie 105:91-8 (2014). Human . PubMed: 24998328
      • Yu Y  et al. Human milk contains novel glycans that are potential decoy receptors for neonatal rotaviruses. Mol Cell Proteomics 13:2944-60 (2014). PubMed: 25048705
      View all Publications for this product

      レビューと Q&A

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      1-2 of 2 Abreviews or Q&A

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Blood Group Lewis b antibody [2-25LE]

       Excellent
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (Human colorectal cell line C170)
      Specification
      Human colorectal cell line C170
      Fixative
      Formaldehyde
      Permeabilization
      No
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      Abcam user community

      Verified customer

      投稿 Feb 09 2011

      Question

      I have ordered a mouse monoclonal [2-25LE] to blood group Lewis B (ab3968) and plan to perform the immunoprecipitation to capture the specific proteins which reacted with this antibody. I would like to inquire whether this antibody is available to do IP. Thanks a lot.

      Read More
      Answer

      Thank you for contacting us.

      We have not tested the antibody ab3968 for immunoprecipitation, and we have not received reports from any customers who have tried it for IP. It is possible it will be useful for IP but we have no data that confirms this.

      We have one other antibody against this target, blood group Lewis B, also monoclonal, but it is untested for IP. Here is a URL link to the datasheet:

      https://www.abcam.com/Blood-Group-Lewis-b-antibody-T218-ab3357.html

      For IP with monoclonal antibodies,a better yield and greater specificity can be obtained by mixing monoclonal antibodies raised against the same protein and then adding the mixed antibodies to the sample, rather than trying to IP with a single monoclonal antibody.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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