Anti-Blood Group Lewis a 抗体 [SPM 522] (ab231432)
Key features and details
- Mouse monoclonal [SPM 522] to Blood Group Lewis a
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-Blood Group Lewis a antibody [SPM 522]
Blood Group Lewis a 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [SPM 522] to Blood Group Lewis a -
由来種
Mouse -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Full length native protein (purified) corresponding to Human Blood Group Lewis a. Mucin isolated from an ovarian cyst.
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ポジティブ・コントロール
- IHC-P: Human colon carcinoma tissue.
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特記事項
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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精製度
Protein A/G purified -
特記事項(精製)
Purified from TCS by Protein A/G. -
ポリ/モノ
モノクローナル -
クローン名
SPM 522 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab231432の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Boil tissue section in Citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes. Incubate with primary antibody for 30 minutes at room temperature. |
特記事項 |
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IHC-P
1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Boil tissue section in Citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes. Incubate with primary antibody for 30 minutes at room temperature. |
ターゲット情報
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関連性
Lewis A (type 1 chain) is expressed in colonic epithelial cells and may be useful for detection of gastrointestinal tumors, pancreatic cancer, and colorectal tumors. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens. -
細胞内局在
Cytoplasmic -
別名
- Lewis A antigen antibody
画像
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Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for blood group Lewis a antibody using ab231432 at 1/200 dilution in immunohistochemcial analysis.
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Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for blood group Lewis a antibody using ab231432 at 1/200 dilution in immunohistochemcial analysis.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab231432 は論文での使用が確認できていません。