Anti-Blood Group Lewis a 抗体 [7LE] (ab3967)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Blood Group Lewis a antibody [7LE] (ab3967)](https://www.abcam.co.jp/ps/products/3/ab3967/Images/Blood-Group-Lewis-a-Primary-antibodies-ab3967-8.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Blood Group Lewis a antibody [7LE] (ab3967)")
Key features and details
- Mouse monoclonal [7LE] to Blood Group Lewis a
- Suitable for: IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG1
製品の概要
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製品名
Anti-Blood Group Lewis a antibody [7LE]
Blood Group Lewis a 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [7LE] to Blood Group Lewis a -
由来種
Mouse -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Mouse, Human -
免疫原
Tissue/ cell preparation (Human). Mucin isolated by size-exclusion chromatography from an ovarian cyst fluid (pure endocervical type according to the Fenoglio.s classification).
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特記事項
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Protein A purified -
特記事項（精製）
Purified from hybridoma culture supernatant. Purity >95% by SDS-PAGE. -
ポリ/モノ
モノクローナル -
クローン名
7LE -
ミエローマ
unknown -
アイソタイプ
IgG1 -
軽鎖の種類
unknown -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
アプリケーション
Our Abpromise guarantee covers the use of ab3967 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. |
ターゲット情報
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関連性
Lewis A (type 1 chain) is expressed in colonic epithelial cells and may be useful for detection of gastrointestinal tumors, pancreatic cancer, and colorectal tumors. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens. -
細胞内局在
Cytoplasmic -
別名
- Lewis A antigen antibody
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Blood Group Lewis a antibody [7LE] (ab3967)This image is courtesy of an Anonymous Abreview.ab3967 staining Blood Group Lewis a in Human colorectal carcinoma tissue by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded tissue sections). The sections were PFA-fixed and subjected to heat-mediated antigen retrieval in citric acid (pH 6.0, citrate buffer) prior to blocking with 2% serum for 20 minutes at 22°C. The primary antibody was used undiluted and incubated with the sample for 1 hour at 22°C. An biotinylated universal ABC kit was used for detection.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Blood Group Lewis a antibody [7LE] (ab3967)](https://www.abcam.co.jp/ps/products/3/ab3967/Images/ab3967-287537-ab3967.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Blood Group Lewis a antibody [7LE] (ab3967)Ab3967 staining Blood group Lewis a in Human pancreas tissue using Immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections).
プロトコール
参考文献 (8)
ab3967 は 8 報の論文で使用されています。
- Purohit S et al. Multiplex glycan bead array for high throughput and high content analyses of glycan binding proteins. Nat Commun 9:258 (2018). PubMed: 29343722
- Tang H et al. Glycans related to the CA19-9 antigen are elevated in distinct subsets of pancreatic cancers and improve diagnostic accuracy over CA19-9. Cell Mol Gastroenterol Hepatol 2:201-221.e15 (2016). PubMed: 26998508
- Zhuang H et al. Overexpression of Lewis y antigen promotes human epididymis protein 4-mediated invasion and metastasis of ovarian cancer cells. Biochimie 105:91-8 (2014). Human . PubMed: 24998328
- Yu Y et al. Human milk contains novel glycans that are potential decoy receptors for neonatal rotaviruses. Mol Cell Proteomics 13:2944-60 (2014). PubMed: 25048705
- Nyström K et al. Virus-induced transcriptional activation of host FUT genes associated with neo-expression of Ley in cytomegalovirus-infected and sialyl-Lex in varicella-zoster virus-infected diploid human cells. Glycobiology 17:355-66 (2007). PubMed: 17202152
- Creuzot-Garcher C et al. Alteration of sialyl Lewis epitope expression in pterygium. Invest Ophthalmol Vis Sci 40:1631-6 (1999). PubMed: 10393028
- Torrado J et al. Lewis antigen alterations in gastric cancer precursors. Gastroenterology 102:424-30 (1992). PubMed: 1732113
- Bara J et al. Immunochemical characterization of mucins. Polypeptide (M1) and polysaccharide (A and Leb) antigens. Biochem J 254:185-93 (1988). PubMed: 2460087
