Anti-Blood Group Lewis a 抗体 [7LE] (ab3967)
Key features and details
- Mouse monoclonal [7LE] to Blood Group Lewis a
- Suitable for: ELISA, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG1
製品の概要
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製品名
Anti-Blood Group Lewis a antibody [7LE]
Blood Group Lewis a 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [7LE] to Blood Group Lewis a -
由来種
Mouse -
アプリケーション
適用あり: ELISA, IHC-P, WBmore details -
種交差性
交差種: Mouse, Human -
免疫原
Tissue/ cell preparation (Human). Mucin isolated by size-exclusion chromatography from an ovarian cyst fluid (pure endocervical type according to the Fenoglio.s classification).
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Protein A purified -
特記事項（精製）
Purified from hybridoma culture supernatant. Purity >95% by SDS-PAGE. -
ポリ/モノ
モノクローナル -
クローン名
7LE -
ミエローマ
unknown -
アイソタイプ
IgG1 -
軽鎖の種類
unknown -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
アプリケーション
Our Abpromise guarantee covers the use of ab3967 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. |
ターゲット情報
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関連性
Lewis A (type 1 chain) is expressed in colonic epithelial cells and may be useful for detection of gastrointestinal tumors, pancreatic cancer, and colorectal tumors. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens. -
細胞内局在
Cytoplasmic -
別名
- Lewis A antigen antibody
画像
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Blood Group Lewis a antibody [7LE] (ab3967)](https://www.abcam.co.jp/ps/products/3/ab3967/Images/Blood-Group-Lewis-a-Primary-antibodies-ab3967-8.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Blood Group Lewis a antibody [7LE] (ab3967)This image is courtesy of an Anonymous Abreview.ab3967 staining Blood Group Lewis a in Human colorectal carcinoma tissue by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded tissue sections). The sections were PFA-fixed and subjected to heat-mediated antigen retrieval in citric acid (pH 6.0, citrate buffer) prior to blocking with 2% serum for 20 minutes at 22°C. The primary antibody was used undiluted and incubated with the sample for 1 hour at 22°C. An biotinylated universal ABC kit was used for detection. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Blood Group Lewis a antibody [7LE] (ab3967)](https://www.abcam.co.jp/ps/products/3/ab3967/Images/ab3967-287537-ab3967.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Blood Group Lewis a antibody [7LE] (ab3967)Ab3967 staining Blood group Lewis a in Human pancreas tissue using Immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections).
プロトコール
参考文献 (8)
ab3967 は 8 報の論文で使用されています。
- Purohit S et al. Multiplex glycan bead array for high throughput and high content analyses of glycan binding proteins. Nat Commun 9:258 (2018). PubMed: 29343722
- Tang H et al. Glycans related to the CA19-9 antigen are elevated in distinct subsets of pancreatic cancers and improve diagnostic accuracy over CA19-9. Cell Mol Gastroenterol Hepatol 2:201-221.e15 (2016). PubMed: 26998508
- Zhuang H et al. Overexpression of Lewis y antigen promotes human epididymis protein 4-mediated invasion and metastasis of ovarian cancer cells. Biochimie 105:91-8 (2014). Human . PubMed: 24998328
- Yu Y et al. Human milk contains novel glycans that are potential decoy receptors for neonatal rotaviruses. Mol Cell Proteomics 13:2944-60 (2014). PubMed: 25048705
- Nyström K et al. Virus-induced transcriptional activation of host FUT genes associated with neo-expression of Ley in cytomegalovirus-infected and sialyl-Lex in varicella-zoster virus-infected diploid human cells. Glycobiology 17:355-66 (2007). PubMed: 17202152
- Creuzot-Garcher C et al. Alteration of sialyl Lewis epitope expression in pterygium. Invest Ophthalmol Vis Sci 40:1631-6 (1999). PubMed: 10393028
- Torrado J et al. Lewis antigen alterations in gastric cancer precursors. Gastroenterology 102:424-30 (1992). PubMed: 1732113
- Bara J et al. Immunochemical characterization of mucins. Polypeptide (M1) and polysaccharide (A and Leb) antigens. Biochem J 254:185-93 (1988). PubMed: 2460087
