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Cdk4 ~ 注目ターゲットのおすすめ製品


ウサギ・モノクローナル 抗体

ターゲット名

交差種

適用・アプリケーション

文献

Abreview
数 評価

製品番号

Cdk4 HuFlow Cyt, ICC/IF, IHC-P, WB81 ab108357
Cdk4 HuFlow Cyt, ICC/IF, IP, WB1 ab68266
Cdk4 HuICC/IF, WB1 ab108355
Cdk4 HuWB ab208505
Cdk4
Alexa Fluor® 488
HuFlow Cyt ab193966
Cdk4
Alexa Fluor® 647
HuICC ab193967

抗体

ターゲット名

交差種

適用・アプリケーション

文献

Abreview
数 評価

製品番号

Cdk4
Rb-PHuWB ab95255
Cdk4Rb-PHu, MsICC/IF, IHC-P, WB3 1ab137675
Cdk4Rb-PHuWB, IP, IHC-P ab226474
抗体一覧

タンパク質・ペプチド

製品名

由来

適用・アプリケーション

製品番号

CDK4 + CCND3 protein (Recombinant)HuFuncS, SDS-PAGE, WBab85646
Cdk4 protein(Recombinant)HuSDS-PAGE, WBab126909
Cdk4 + Cyclin D1 protein(Recombinant)HuFuncS, SDS-PAGEab55695
Cdk4 protein (Recombinant)HuSDS-PAGEab134542
タンパク質・ペプチド一覧

生理活性物質

製品名

解説

製品番号

Indole-3-carbinol (I3C)Phytochemical. Anticancer agent.ab143132
FascaplysinPotent, selective ATP-competitve CDK4 inhibitorab146177

Cdk4 について

Function
機能
Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
Involvement in disease
疾患関連性
Defects in CDK4 are a cause of susceptibility to cutaneous malignant melanoma type 3 (CMM3) [MIM:609048]. Malignant melanoma is a malignant neoplasm of melanocytes, arising de novo or from a pre-existing benign nevus, which occurs most often in the skin but also may involve other sites.
Sequence similarities
配列類似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.
Post-translational modifications
翻訳後修飾
Phosphorylation at Thr-172 is required for enzymatic activity. Phosphorylated, in vitro, at this site by CCNH-CDK7, but, in vivo, appears to be phosphorylated by a proline-directed kinase. In the cyclin D-CDK4-CDKN1B complex, this phosphorylation and consequent CDK4 enzyme activity, is dependent on the tyrosine phosphorylation state of CDKN1B. Thus, in proliferating cells, CDK4 within the complex is phosphorylated on Thr-172 in the T-loop. In resting cells, phosphorylation on Thr-172 is prevented by the non-tyrosine-phosphorylated form of CDKN1B.
Cellular localization
細胞内局在性
Cytoplasm. Nucleus. Membrane. Cytoplasmic when non-complexed. Forms a cyclin D-CDK4 complex in the cytoplasm as cells progress through G(1) phase. The complex accumulates on the nuclear membrane and enters the nucleus on transition from G(1) to S phase. Also present in nucleoli and heterochromatin lumps. Colocalizes with RB1 after release into the nucleus.

関連リンク


Cancer
ポータル

注目
ターゲット

Cdk2

Cyclin

Ki67

PCNA

細胞周期の制御と
Cdk、Cyclin

細胞増殖研究ガイド

G1/S パスウェイ

キーワード「Cell cycle



Alexa Fluor® is a registered trademark of Life Technologies. Alexa Fluor® dye conjugates contain(s) technology licensed to Abcam by Life Technologies.


ウサギ・モノクローナル 抗体

ターゲット名

交差種

適用・アプリケーション

文献

Abreview
数 評価

製品番号

Cdk4 HuFlow Cyt, ICC/IF, IHC-P, WB81 ab108357
Cdk4 HuFlow Cyt, ICC/IF, IP, WB1 ab68266
Cdk4 HuICC/IF, WB1 ab108355
Cdk4 HuWB ab208505
Cdk4
Alexa Fluor® 488
HuFlow Cyt ab193966
Cdk4
Alexa Fluor® 647
HuICC ab193967

抗体

ターゲット名

交差種

適用・アプリケーション

文献

Abreview
数 評価

製品番号

Cdk4
Rb-PHuWB ab95255
Cdk4Rb-PHu, MsICC/IF, IHC-P, WB3 1ab137675
Cdk4Rb-PHuWB, IP, IHC-P ab226474
抗体一覧

タンパク質・ペプチド

製品名

由来

適用・アプリケーション

製品番号

CDK4 + CCND3 protein (Recombinant)HuFuncS, SDS-PAGE, WBab85646
Cdk4 protein(Recombinant)HuSDS-PAGE, WBab126909
Cdk4 + Cyclin D1 protein(Recombinant)HuFuncS, SDS-PAGEab55695
Cdk4 protein (Recombinant)HuSDS-PAGEab134542
タンパク質・ペプチド一覧

生理活性物質

製品名

解説

製品番号

Indole-3-carbinol (I3C)Phytochemical. Anticancer agent.ab143132
FascaplysinPotent, selective ATP-competitve CDK4 inhibitorab146177

Cdk4 について

Function
機能
Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
Involvement in disease
疾患関連性
Defects in CDK4 are a cause of susceptibility to cutaneous malignant melanoma type 3 (CMM3) [MIM:609048]. Malignant melanoma is a malignant neoplasm of melanocytes, arising de novo or from a pre-existing benign nevus, which occurs most often in the skin but also may involve other sites.
Sequence similarities
配列類似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.
Post-translational modifications
翻訳後修飾
Phosphorylation at Thr-172 is required for enzymatic activity. Phosphorylated, in vitro, at this site by CCNH-CDK7, but, in vivo, appears to be phosphorylated by a proline-directed kinase. In the cyclin D-CDK4-CDKN1B complex, this phosphorylation and consequent CDK4 enzyme activity, is dependent on the tyrosine phosphorylation state of CDKN1B. Thus, in proliferating cells, CDK4 within the complex is phosphorylated on Thr-172 in the T-loop. In resting cells, phosphorylation on Thr-172 is prevented by the non-tyrosine-phosphorylated form of CDKN1B.
Cellular localization
細胞内局在性
Cytoplasm. Nucleus. Membrane. Cytoplasmic when non-complexed. Forms a cyclin D-CDK4 complex in the cytoplasm as cells progress through G(1) phase. The complex accumulates on the nuclear membrane and enters the nucleus on transition from G(1) to S phase. Also present in nucleoli and heterochromatin lumps. Colocalizes with RB1 after release into the nucleus.

関連リンク


Cancer
ポータル

注目
ターゲット

Cdk2

Cyclin

Ki67

PCNA

細胞周期の制御と
Cdk、Cyclin

細胞増殖研究ガイド

G1/S パスウェイ

キーワード「Cell cycle



Alexa Fluor® is a registered trademark of Life Technologies. Alexa Fluor® dye conjugates contain(s) technology licensed to Abcam by Life Technologies.

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