Welcome to the Abcam blog

Notch ~ 注目ターゲットのおすすめ製品


ウサギ・モノクローナル 抗体

ターゲット名

交差種

適用・アプリケーション

文献

Abreview
数 評価

製品番号

Notch1 Ms, Hu, CowFlow Cyt, ICC/IF, IHC-P, WB329 ab52627
Notch1
Alexa Fluor® 647
Ms, Hu, CowICC/IF ab194122
Notch1 HuWB ab194123
NOTCH4Hu, Ms, RatFlow Cyt, ICC/IF, IP, WB ab184742
NOTCH4
BSA and Azide free
Hu, Ms, RatFlow Cyt, ICC/IF, IP, WB ab184742
NOTCH4 Ms, HuFlow Cyt, WB ab166605
NOTCH4WB ab170951

抗体

ターゲット名

交差種

適用・アプリケーション

文献

Abreview
数 評価

製品番号

Notch1Rb-PHu, MsChIP, ICC/IF, IHC-FoFr, IHC-Fr, IHC-P, WB2916 ab27526
Notch1Rb-P Hu, MsFlow Cyt, ICC/IF, IHC-Fr, IHC-P, IP, WB3 ab65297
Notch1Rb-PHu, Ms, RatELISA, ICC/IF, IHC-P, WB8 ab52301
activated Notch1Rb-PHu, MsFlow Cyt, ICC/IF, IHC-Fr, IHC-P, WB6322ab8925
Notch1 intracellular domainRb-P Hu, A leptIHC-Fr, WB35 ab83232
Notch 2Rb-PHuELISA, ICC/IF, IHC-P, WB142 ab8926
NOTCH3Rb-PHu, Ms, RatICC, ICC/IF, IHC-FoFr, IHC-Fr, IHC-P, WB2314 ab23426
抗体一覧

タンパク質・ペプチド

ターゲット名

由来

適用・アプリケーション

製品番号

Notch1 protein (Recombinant)HuELISA, SDS-PAGE, WBab114178
Human Notch2 protein(Recombinant)HuELISA, SDS-PAGE, WBab114827
Human NOTCH3 protein (Recombinant)HuELISA, SDS-PAGE, WBab114234
タンパク質・ペプチド一覧

Notch について

Notch1
Function
機能
Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May be important for normal lymphocyte function. In altered form, may contribute to transformation or progression in some T-cell neoplasms. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. May be important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, may function as a receptor for neuronal DNER and may be involved in the differentiation of Bergmann glia.
Tissue specificity
組織特異性
In fetal tissues most abundant in spleen, brain stem and lung. Also present in most adult tissues where it is found mainly in lymphoid tissues.
Involvement in disease
関連疾患
Defects in NOTCH1 are a cause of bicuspid aortic valve (BAV) [MIM:109730]. A common defect in the aortic valve in which two rather than three leaflets are present. It is often associated with aortic valve calcification and insufficiency. In extreme cases, the blood flow may be so restricted that the left ventricle fails to grow, resulting in hypoplastic left heart syndrome.
Post-translational modifications
翻訳後修飾
Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane. Phosphorylated. O-glycosylated on the EGF-like domains. Contains both O-linked fucose and O-linked glucose. Ubiquitinated; undergoes 'Lys-29'-linked polyubiquitination catalyzed by ITCH.
Cellular localization
細胞内局在性
Cell membrane and Nucleus. Following proteolytical processing NICD is translocated to the nucleus.

Notch2
Function
機能
Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity). Involved in bone remodeling and homeostasis. In collaboration with RELA/p65 enhances NFATc1 promoter activity and positively regulates RANKL-induced osteoclast differentiation. Positively regulates self-renewal of liver cancer cells (PubMed:25985737).
Tissue specificity
組織特異性
Expressed in the brain, heart, kidney, lung, skeletal muscle and liver. Ubiquitously expressed in the embryo.
Involvement in disease
関連疾患
  • Alagille syndrome 2
  • Hajdu-Cheney syndrome
Post-translational modifications
翻訳後修飾
Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane. Hydroxylated by HIF1AN. Can be either O-glucosylated or O-xylosylated at Ser-613 by POGLUT1.
Cellular localization
細胞内局在性
Cell membrane and Nucleus. Cytoplasm. Following proteolytical processing NICD is translocated to the nucleus. Retained at the cytoplasm by C8orf4 (PubMed:25985737).

Notch3
Function
機能
Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.
Tissue specificity
組織特異性
Ubiquitously expressed in fetal and adult tissues.
Involvement in disease
関連疾患
Defects in NOTCH3 are the cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) [MIM:125310]. CADASIL causes a type of stroke and dementia of which key features include recurrent subcortical ischemic events and vascular dementia. The disorder affects relatively young adults of both sexes. Mutations affect highly conserved cysteine residues within epidermal growth factor (EGF)-like repeat domains in the extracellular part of the receptor.
Post-translational modifications
翻訳後修飾
Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane. Phosphorylated.
Cellular localization
細胞内局在性
Cell membrane and Nucleus. Following proteolytical processing NICD is translocated to the nucleus.

Notch4
Function
機能
Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May regulate branching morphogenesis in the developing vascular system.
Tissue specificity
組織特異性
Highly expressed in the heart, moderately in the lung and placenta and at low levels in the liver, skeletal muscle, kidney, pancreas, spleen, lymph node, thymus, bone marrow and fetal liver. No expression was seen in adult brain or peripheral blood leukocytes.
Post-translational modifications
翻訳後修飾
Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane. Phosphorylated.
Cellular localization
細胞内局在性
Cell membrane and Nucleus. Following proteolytical processing NICD is translocated to the nucleus.

関連リンク


Neuroscience
ポータル

注目のターゲット

神経細胞マーカー

Alexa FluorR is a registered trademark of Life Technologies. Alexa FluorR dye conjugates contain(s) technology licensed to Abcam by Life Technologies.



ウサギ・モノクローナル 抗体

ターゲット名

交差種

適用・アプリケーション

文献

Abreview
数 評価

製品番号

Notch1 Ms, Hu, CowFlow Cyt, ICC/IF, IHC-P, WB329 ab52627
Notch1
Alexa Fluor® 647
Ms, Hu, CowICC/IF ab194122
Notch1 HuWB ab194123
NOTCH4Hu, Ms, RatFlow Cyt, ICC/IF, IP, WB ab184742
NOTCH4
BSA and Azide free
Hu, Ms, RatFlow Cyt, ICC/IF, IP, WB ab184742
NOTCH4 Ms, HuFlow Cyt, WB ab166605
NOTCH4WB ab170951

抗体

ターゲット名

交差種

適用・アプリケーション

文献

Abreview
数 評価

製品番号

Notch1Rb-PHu, MsChIP, ICC/IF, IHC-FoFr, IHC-Fr, IHC-P, WB2916 ab27526
Notch1Rb-P Hu, MsFlow Cyt, ICC/IF, IHC-Fr, IHC-P, IP, WB3 ab65297
Notch1Rb-PHu, Ms, RatELISA, ICC/IF, IHC-P, WB8 ab52301
activated Notch1Rb-PHu, MsFlow Cyt, ICC/IF, IHC-Fr, IHC-P, WB6322ab8925
Notch1 intracellular domainRb-P Hu, A leptIHC-Fr, WB35 ab83232
Notch 2Rb-PHuELISA, ICC/IF, IHC-P, WB142 ab8926
NOTCH3Rb-PHu, Ms, RatICC, ICC/IF, IHC-FoFr, IHC-Fr, IHC-P, WB2314 ab23426
抗体一覧

タンパク質・ペプチド

ターゲット名

由来

適用・アプリケーション

製品番号

Notch1 protein (Recombinant)HuELISA, SDS-PAGE, WBab114178
Human Notch2 protein(Recombinant)HuELISA, SDS-PAGE, WBab114827
Human NOTCH3 protein (Recombinant)HuELISA, SDS-PAGE, WBab114234
タンパク質・ペプチド一覧

Notch について

Notch1
Function
機能
Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May be important for normal lymphocyte function. In altered form, may contribute to transformation or progression in some T-cell neoplasms. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. May be important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, may function as a receptor for neuronal DNER and may be involved in the differentiation of Bergmann glia.
Tissue specificity
組織特異性
In fetal tissues most abundant in spleen, brain stem and lung. Also present in most adult tissues where it is found mainly in lymphoid tissues.
Involvement in disease
関連疾患
Defects in NOTCH1 are a cause of bicuspid aortic valve (BAV) [MIM:109730]. A common defect in the aortic valve in which two rather than three leaflets are present. It is often associated with aortic valve calcification and insufficiency. In extreme cases, the blood flow may be so restricted that the left ventricle fails to grow, resulting in hypoplastic left heart syndrome.
Post-translational modifications
翻訳後修飾
Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane. Phosphorylated. O-glycosylated on the EGF-like domains. Contains both O-linked fucose and O-linked glucose. Ubiquitinated; undergoes 'Lys-29'-linked polyubiquitination catalyzed by ITCH.
Cellular localization
細胞内局在性
Cell membrane and Nucleus. Following proteolytical processing NICD is translocated to the nucleus.

Notch2
Function
機能
Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity). Involved in bone remodeling and homeostasis. In collaboration with RELA/p65 enhances NFATc1 promoter activity and positively regulates RANKL-induced osteoclast differentiation. Positively regulates self-renewal of liver cancer cells (PubMed:25985737).
Tissue specificity
組織特異性
Expressed in the brain, heart, kidney, lung, skeletal muscle and liver. Ubiquitously expressed in the embryo.
Involvement in disease
関連疾患
  • Alagille syndrome 2
  • Hajdu-Cheney syndrome
Post-translational modifications
翻訳後修飾
Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane. Hydroxylated by HIF1AN. Can be either O-glucosylated or O-xylosylated at Ser-613 by POGLUT1.
Cellular localization
細胞内局在性
Cell membrane and Nucleus. Cytoplasm. Following proteolytical processing NICD is translocated to the nucleus. Retained at the cytoplasm by C8orf4 (PubMed:25985737).

Notch3
Function
機能
Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.
Tissue specificity
組織特異性
Ubiquitously expressed in fetal and adult tissues.
Involvement in disease
関連疾患
Defects in NOTCH3 are the cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) [MIM:125310]. CADASIL causes a type of stroke and dementia of which key features include recurrent subcortical ischemic events and vascular dementia. The disorder affects relatively young adults of both sexes. Mutations affect highly conserved cysteine residues within epidermal growth factor (EGF)-like repeat domains in the extracellular part of the receptor.
Post-translational modifications
翻訳後修飾
Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane. Phosphorylated.
Cellular localization
細胞内局在性
Cell membrane and Nucleus. Following proteolytical processing NICD is translocated to the nucleus.

Notch4
Function
機能
Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May regulate branching morphogenesis in the developing vascular system.
Tissue specificity
組織特異性
Highly expressed in the heart, moderately in the lung and placenta and at low levels in the liver, skeletal muscle, kidney, pancreas, spleen, lymph node, thymus, bone marrow and fetal liver. No expression was seen in adult brain or peripheral blood leukocytes.
Post-translational modifications
翻訳後修飾
Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane. Phosphorylated.
Cellular localization
細胞内局在性
Cell membrane and Nucleus. Following proteolytical processing NICD is translocated to the nucleus.

関連リンク


Neuroscience
ポータル

注目のターゲット

神経細胞マーカー

Alexa FluorR is a registered trademark of Life Technologies. Alexa FluorR dye conjugates contain(s) technology licensed to Abcam by Life Technologies.


コメント (承認されるまでコメントは表示されません)
登録