Anti-BLBP 抗体 [EPR24033-13] (ab279649)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24033-13] to BLBP
- Suitable for: WB, IHC-Fr, IHC-P, Flow Cyt (Intra), IP, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-BLBP antibody [EPR24033-13]
BLBP 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR24033-13] to BLBP -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Fr, IHC-P, Flow Cyt (Intra), IP, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Mouse brain and cerebellum tissue lysate. Human cerebellum tissue lysate. Rat brain cortex and cerebellum tissue lysate. IHC-P: Human, mouse and rat cerebrum tissue. Human astrocytoma. IHC-Fr: Mouse cerebellum tissue. ICC/IF: Mouse and rat primary neural/glia cells. Flow Cyt (intra): Mouse and rat primary neural/glia cells. IP: Mouse cerebellum tissue lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR24033-13 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab279649の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000. Predicted molecular weight: 14 kDa.
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IHC-Fr | (2) |
1/50.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) |
IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Flow Cyt (Intra) |
1/500.
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IP |
1/30.
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ICC/IF |
1/250.
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特記事項 |
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WB
1/1000. Predicted molecular weight: 14 kDa. |
IHC-Fr
1/50. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) |
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Flow Cyt (Intra)
1/500. |
IP
1/30. |
ICC/IF
1/250. |
ターゲット情報
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機能
B-FABP could be involved in the transport of a so far unknown hydrophobic ligand with potential morphogenic activity during CNS development. It is required for the establishment of the radial glial fiber system in developing brain, a system that is necessary for the migration of immature neurons to establish cortical layers. -
組織特異性
Expressed in brain and other neural tissues. -
配列類似性
Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family. -
ドメイン
Forms a beta-barrel structure that accommodates the hydrophobic ligand in its interior. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 2173 Human
- Entrez Gene: 12140 Mouse
- Entrez Gene: 80841 Rat
- Omim: 602965 Human
- SwissProt: O15540 Human
- SwissProt: P51880 Mouse
- SwissProt: P55051 Rat
- Unigene: 26770 Human
see all -
別名
- B FABP antibody
- B-FABP antibody
- BFABP antibody
see all
画像
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All lanes : Anti-BLBP antibody [EPR24033-13] (ab279649) at 1/1000 dilution
Lane 1 : Human cerebellum tissue lysate
Lane 2 : Human spleen tissue lysate
Lane 3 : Human kidney tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: spleen, kidney (PMID: 16618771)
Exposure time: 10 seconds
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Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling BLBP with ab279649 at 1/500 dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human cerebrum (PMID: 16623952). The section was incubated with ab279649 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebellum tissue labeling BLBP with ab279649 at 1/50 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse cerebellum, which partially co-stained with GFAP (Red) is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1:1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).dilution. -
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse primary neural/glia cells labelling BLBP with ab279649 at 1/250 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing positive staining in mouse primary gliocyte. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection is observed. Anti-Glial Fibrillary Acidic Protein (GFAP) mouse monoclonal antibody was used to counterstain tubulin at 1/100 dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at a 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).
Negative control 1: ab279649 at a 1/250 dilution followed by ab150120 at a 1/1000 dilution.
Negative control 2: Anti-Glial Fibrillary Acidic Protein (GFAP) mouse monoclonal antibody at a 1/100 dilution followed by ab150077 at a 1/1000 dilution.
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Flow cytometric analysis of 4% paraformaldehyde fixed 0.1% Tween-20 permeabilized mouse primary neural/glia cells labeling BLBP with ab279649 at 1/500 dilution (Right panel) compared with Rabbit monoclonal IgG Isotype Control (ab172730) (Left panel). Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) at 1/2000 dilution was used as the secondary antibody.
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BLBP was immunoprecipitated from 0.35 mg mouse cerebellum tissue lysate 10 µg with ab279649 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab279649 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Mouse cerebellum tissue lysate 10 µg
Lane 2: ab279649 IP in mouse cerebellum tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab279649 in mouse cerebellum tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
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Immunohistochemical analysis of paraffin-embedded human astrocytoma labeling BLBP with ab279649 at 1/500 dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human astrocytoma (PMID: 16623952). The section was incubated with ab279649 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling BLBP with ab279649 at 1/500 dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab279649 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Negative control: no staining on human kidney (PMID: 9375786).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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All lanes : Anti-BLBP antibody [EPR24033-13] (ab279649) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse cerebellum tissue lysate
Lane 3 : Mouse spleen tissue lysate
Lane 4 : Mouse kidney tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: spleen, kidney (PMID: 16618771)
Exposure time: 6 seconds
-
Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling BLBP with ab279649 at 1/2000 dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse cerebrum. The section was incubated with ab279649 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling BLBP with ab279649 at 1/2000 dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab279649 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Negative control: no staining on mouse kidney.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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All lanes : Anti-BLBP antibody [EPR24033-13] (ab279649) at 1/1000 dilution
Lane 1 : Rat brain cortex tissue lysate
Lane 2 : Rat cerebellum tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure times:
Lane 1: 3 seconds
Lane 2: 26 seconds
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Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling BLBP with ab279649 at 1/2000 dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat cerebrum. The section was incubated with ab279649 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized rat primary neural/glia cells labelling BLBP with ab279649 at 1/250 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing positive staining in rat primary gliocyte. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection is observed. Anti-Glial Fibrillary Acidic Protein (GFAP) mouse monoclonal antibody was used to counterstain tubulin at 1/100 dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at a 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).
Negative control 1: ab279649 at a 1/250 dilution followed by ab150120 at a 1/1000 dilution.
Negative control 2: Anti-Glial Fibrillary Acidic Protein (GFAP) mouse monoclonal antibody at a 1/100 dilution followed by ab150077 at a 1/1000 dilution.
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Flow cytometric analysis of 4% paraformaldehyde fixed 0.1% Tween-20 permeabilized rat primary neural/glia cells labeling BLBP with ab279649 at 1/500 dilution (Right panel) compared with Rabbit monoclonal IgG Isotype Control (ab172730) (Left panel). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077 at 1/2000 dilution was used as the secondary antibody.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (2)
ab279649 は 2 報の論文で使用されています。
- Mason AJ et al. Sympathetic neurons secrete retrogradely transported TrkA on extracellular vesicles. Sci Rep 13:3657 (2023). PubMed: 36871060
- Zhu Y et al. Ameliorative Effect of Ethanolic Echinacea purpurea against Hyperthyroidism-Induced Oxidative Stress via AMRK and PPAR Signal Pathway Using Transcriptomics and Network Pharmacology Analysis. Int J Mol Sci 24:N/A (2022). PubMed: 36613632