Anti-BirA 抗体 [6C4c7] - BSA and Azide free (ab269579)
![Immunocytochemistry/ Immunofluorescence - Anti-BirA antibody [6C4c7] - BSA and Azide free (ab269579)](https://www.abcam.co.jp/ps/products/269/ab269579/Images/ab269579-381334-antibira6c4c7icchek293transfecteddox.jpg "Immunocytochemistry/ Immunofluorescence - Anti-BirA antibody [6C4c7] - BSA and Azide free (ab269579)")
Key features and details
- Mouse monoclonal [6C4c7] to BirA - BSA and Azide free
- Suitable for: WB, ICC/IF
- Reacts with: Escherichia coli
- Isotype: IgG1
製品の概要
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製品名
Anti-BirA antibody [6C4c7] - BSA and Azide free
BirA 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [6C4c7] to BirA - BSA and Azide free -
由来種
Mouse -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Escherichia coli -
免疫原
Recombinant fragment (GST-tag) within Escherichia coli BirA aa 1-150 (N terminal). The exact sequence is proprietary. Glutathione-S-transferase fused to E.coli BirA R118G
Database link: P06709 -
ポジティブ･コントロール
- WB: BirA-transfected HEK293T whole cell lysates (induced). ICC: BirA transfected HEK293T (induced) cells
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
ab269579 is the carrier-free version of ab232732. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
6C4c7 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab269579 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa.
|
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| ICC/IF |
Use a concentration of 5 µg/ml.
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| 特記事項 |
|---|
|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa. |
|
ICC/IF
Use a concentration of 5 µg/ml. |
ターゲット情報
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関連性
BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'-adenylate and transfers the biotin moiety to biotin-accepting proteins. -
参照データベース
- Entrez Gene: 4491453 Escherichia coli
- SwissProt: P06709 Escherichia coli
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別名
- Bifunctional protein birA antibody
- Bio R antibody
- BioR antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-BirA antibody [6C4c7] - BSA and Azide free (ab269579)
This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab232732)
ab232732 staining BirA in normal HEK293 cells, BirA transfected HEK293T cells (uninduced) and BirA transfected HEK293T cells induced with DOX. The cells were fixed with 100% MeOH (5min), permeabilized with 0.1%PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab232732 at 5μg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolor red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Cell samples kindly provided by the Brian Burke laboratory, A-Star Institute, Singapore.
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![Western blot - Anti-BirA antibody [6C4c7] - BSA and Azide free (ab269579)](https://www.abcam.co.jp/ps/products/269/ab269579/Images/ab269579-368356-AntiBirA-antibody-6C4c7-BSA-Azide-free--western-blot-BirAtransfected-HEK293T-induced.jpg)
Western blot - Anti-BirA antibody [6C4c7] - BSA and Azide free (ab269579)All lanes : Anti-BirA antibody [6C4c7] (ab232732) at 1 µg/ml
Lane 1 : HEK293 whole cell lysate
Lane 2 : HEK293T whole cell lysate
Lane 3 : BirA-SLAP75 transfected HEK293T whole cell lysate, mock induced
Lane 4 : BirA-SLAP75 transfected HEK293T whole cell lysate, DOX induced
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 35 kDaThis blot was produced using a 4-12% Bis-tris under the MOPS buffer system. The gel was run at 200V for 55 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was blocked for an hour using 5% milk before ab232732 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at a 1µg/ml concentration and 1/20,000 dilution respectively. Antibody binding was detected using Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.
Cell samples kindly provided by the Brian Burke laboratory, A-Star Institute, Singapore.
This image was produced using the same antibody clone but in a different formulation ab232732, PBS and sodium azide.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
参考文献 (0)
ab269579 は論文での使用が確認できていません。
