Biotinylated Goat Anti-Rabbit IgG (H+L) (Ready to use) (ab64256)
製品の概要
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製品名
Biotinylated Goat Anti-Rabbit IgG (H+L) (Ready to use)
IgG ihc 試薬 製品一覧 -
アプリケーション
適用あり: IHC-Pmore details -
特記事項
Staining protocol- Deparaffinize and rehydrate formalin-fixed paraffin-embedded tissue section.
- Add appropriate hydrogen peroxide blocking solution to cover the sections. Incubate for 10 minutes. Wash 2 times in buffer.
- Perform appropriate pretreatment if required. Wash 3 times in buffer.
- Apply appropriate protein blocking solution and incubate for 5 minutes at room temperature to block non specific background staining. Wash 1 time in buffer.
- Apply primary antibody and incubate according to manufacturer's protocol.
- Wash 4 times in buffer. Apply Ready to use Biotinylated Goat anti Rabbit IgG (ab64256) solution and incubate for 10-15 minutes at room temperature. Wash 4 times in buffer.
- Apply enzyme labelled streptavidin and incubate according to manufacturer’s protocol.
- Rinse 4 times in buffer. Place slide in appropriate chromogenic substrate and incubate until desired reaction is achieved. Rinse 4 times in buffer.
- Add enough drops of Haematoxylin to cover the section. Incubate for 1 minute.
- Rinse 7-8 times in tap water. Add Mounting Medium to cover the section.
This reagent is intended to be used in a labeled streptavidin biotin immunoenzymatic antigen detection system. This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody which reacts with the primary antibody, enzyme labeled streptavidin, and substrate chromogen.
製品の特性
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製品の状態
Liquid -
保存方法
Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.0985% Sodium azide
Constituent: PBS -
Concentration information loading...
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IHC 試薬 備考
This reagent is intended to be used in a labeled streptavidin biotin immunoenzymatic antigen detection system. This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody which reacts with the primary antibody, enzyme labeled streptavidin, and substrate chromogen. -
研究分野
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細胞内局在
Secreted -
別名
- Ig gamma 1 chain C region
- Ig gamma 2 chain C region
- Ig gamma 3 chain C region
see all -
細胞内局在
Secreted
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab64256の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use at an assay dependent dilution.
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特記事項 |
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IHC-P
Use at an assay dependent dilution. |
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (101)
ab64256 は 101 報の論文で使用されています。
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- Ashraf UM et al. Intrarenal Dopaminergic System Is Dysregulated in SS-Resp18mutant Rats. Biomedicines 11:N/A (2023). PubMed: 36672619
- Zhang Y et al. Copper Induces Cognitive Impairment in Mice via Modulation of Cuproptosis and CREB Signaling. Nutrients 15:N/A (2023). PubMed: 36839332
- Becker V et al. Hypoxia-induced downregulation of microRNA-186-5p in endothelial cells promotes non-small cell lung cancer angiogenesis by upregulating protein kinase C alpha. Mol Ther Nucleic Acids 31:421-436 (2023). PubMed: 36845338